Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   HUW50_RS00830 Genome accession   NZ_CP055263
Coordinates   163156..164223 (-) Length   355 a.a.
NCBI ID   WP_185653620.1    Uniprot ID   -
Organism   Metabacillus sp. KUDC1714     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 158156..169223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUW50_RS00780 (HUW50_00780) - 158544..158789 (-) 246 WP_185653612.1 hypothetical protein -
  HUW50_RS00785 (HUW50_00785) - 158965..159279 (+) 315 WP_066335043.1 DUF3889 domain-containing protein -
  HUW50_RS00790 (HUW50_00790) - 159478..159669 (-) 192 WP_066335042.1 YqzE family protein -
  HUW50_RS00795 (HUW50_00795) - 159724..160218 (-) 495 WP_185653613.1 shikimate kinase -
  HUW50_RS00800 (HUW50_00800) comGG 160262..160642 (-) 381 WP_185653614.1 competence type IV pilus minor pilin ComGG -
  HUW50_RS00805 (HUW50_00805) comGF 160642..161175 (-) 534 WP_260445640.1 competence type IV pilus minor pilin ComGF -
  HUW50_RS00810 (HUW50_00810) comGE 161066..161392 (-) 327 WP_185653616.1 competence type IV pilus minor pilin ComGE -
  HUW50_RS00815 (HUW50_00815) comGD 161379..161831 (-) 453 WP_185653617.1 competence type IV pilus minor pilin ComGD -
  HUW50_RS00820 (HUW50_00820) comGC 161806..162123 (-) 318 WP_185653618.1 competence type IV pilus major pilin ComGC Machinery gene
  HUW50_RS00825 (HUW50_00825) comGB 162132..163166 (-) 1035 WP_185653619.1 competence type IV pilus assembly protein ComGB -
  HUW50_RS00830 (HUW50_00830) comGA 163156..164223 (-) 1068 WP_185653620.1 competence type IV pilus ATPase ComGA Machinery gene
  HUW50_RS00840 (HUW50_00840) - 164899..165147 (+) 249 WP_066334907.1 DUF2626 domain-containing protein -
  HUW50_RS00845 (HUW50_00845) - 165200..165832 (-) 633 WP_185653621.1 MBL fold metallo-hydrolase -
  HUW50_RS00850 (HUW50_00850) - 166127..166300 (+) 174 WP_066334902.1 DUF2759 domain-containing protein -
  HUW50_RS00855 (HUW50_00855) - 166348..166665 (-) 318 WP_185653622.1 MTH1187 family thiamine-binding protein -
  HUW50_RS00860 (HUW50_00860) - 166682..167797 (-) 1116 WP_185653623.1 hypothetical protein -
  HUW50_RS00865 (HUW50_00865) - 167992..169122 (-) 1131 WP_185653624.1 M14 family metallocarboxypeptidase -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 40259.77 Da        Isoelectric Point: 8.6624

>NTDB_id=457277 HUW50_RS00830 WP_185653620.1 163156..164223(-) (comGA) [Metabacillus sp. KUDC1714]
MQTIEQLSERLIEEACALRASDIHIVPKEQETLIHFRVDDDLIEKHSMKKEICERIISHLKFLASMDIGERRRPQNGALS
ISTTNINVHLRLSTLPTIHEESLVIRILPQEKIPPITQLSLFPATTNKLLSILNHSHGLMVFTGPTGSGKTTTLYSLIHY
AKRHFNRNIITLEDPVETKSAEVLQVQVNEKAGITYAAGLKAILRHDPDIIMVGEIRDRETAQIAIRASLTGHLVLSTMH
TRDAKGAIYRLLEFDVSLSEIEQTLIAVSAQRLVQLKCPFCEGECSPFCKKLRKKRRLSVYELLYGKNLTAVVKEAKGEV
SAYSYPMLKDVIKKGIALGYLYPNSYDRWVFRYED

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=457277 HUW50_RS00830 WP_185653620.1 163156..164223(-) (comGA) [Metabacillus sp. KUDC1714]
ATGCAAACCATTGAACAGCTGAGTGAACGACTAATAGAAGAAGCTTGTGCATTACGTGCTTCAGATATACACATTGTTCC
TAAAGAACAAGAAACGTTAATTCATTTTAGGGTAGATGACGACTTAATCGAAAAACACTCGATGAAAAAAGAGATATGTG
AGCGAATTATTTCTCATTTGAAATTTCTTGCTTCAATGGATATTGGCGAAAGGAGACGACCACAAAACGGAGCACTATCT
ATCTCAACAACAAATATTAATGTGCATCTCAGGTTATCAACACTACCTACCATTCATGAGGAAAGCCTTGTTATCCGAAT
TTTACCTCAAGAGAAAATTCCCCCAATTACACAATTATCCTTATTTCCTGCTACAACAAACAAATTGCTCTCAATTTTAA
ACCATTCTCACGGGTTAATGGTATTTACCGGTCCAACCGGCTCAGGTAAGACAACCACTTTATATTCACTTATCCACTAT
GCAAAGCGTCATTTTAACCGAAATATTATTACGCTAGAAGACCCAGTTGAAACAAAAAGTGCAGAAGTACTGCAAGTTCA
AGTTAATGAAAAAGCTGGAATTACCTACGCTGCAGGGTTAAAGGCAATTTTAAGACACGATCCGGATATAATAATGGTTG
GTGAAATTCGAGATCGTGAAACAGCCCAGATTGCGATTAGAGCTAGTCTAACAGGCCATTTAGTACTCTCTACAATGCAT
ACAAGAGATGCAAAGGGTGCGATATACAGGCTACTTGAATTCGATGTAAGTCTATCTGAAATAGAACAAACATTGATCGC
AGTTTCAGCTCAGCGCCTCGTACAGCTTAAGTGCCCATTTTGTGAAGGTGAATGTTCTCCTTTTTGTAAGAAATTAAGGA
AAAAAAGACGTTTAAGTGTATATGAATTGCTTTATGGTAAAAACCTTACAGCGGTTGTAAAGGAAGCAAAAGGAGAAGTT
TCAGCATATTCTTATCCAATGTTAAAGGATGTGATCAAAAAAGGCATTGCACTTGGATACCTATATCCTAATTCATATGA
CCGCTGGGTGTTTCGATATGAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

66.573

100

0.668
  pilB Glaesserella parasuis strain SC1401

39.045

100

0.392