Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HU257_RS11325 Genome accession   NZ_CP055230
Coordinates   2505367..2506737 (+) Length   456 a.a.
NCBI ID   WP_178306309.1    Uniprot ID   -
Organism   Enterococcus hirae strain 13152     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2500367..2511737
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU257_RS11300 (HU257_11300) - 2501682..2501999 (+) 318 WP_010737260.1 hypothetical protein -
  HU257_RS11305 (HU257_11305) - 2502088..2502432 (-) 345 WP_010720642.1 hypothetical protein -
  HU257_RS11310 (HU257_11310) - 2502536..2503780 (-) 1245 WP_034700060.1 glutamate-5-semialdehyde dehydrogenase -
  HU257_RS11315 (HU257_11315) proB 2503777..2504583 (-) 807 WP_010720640.1 glutamate 5-kinase -
  HU257_RS11320 (HU257_11320) - 2504785..2505315 (+) 531 WP_178306308.1 dUTP diphosphatase -
  HU257_RS11325 (HU257_11325) radA 2505367..2506737 (+) 1371 WP_178306309.1 DNA repair protein RadA Machinery gene
  HU257_RS11330 (HU257_11330) - 2506771..2507931 (+) 1161 WP_010720637.1 PIN/TRAM domain-containing protein -
  HU257_RS11335 (HU257_11335) gltX 2508148..2509608 (+) 1461 WP_010720636.1 glutamate--tRNA ligase -
  HU257_RS11340 (HU257_11340) epsC 2509921..2510457 (+) 537 WP_010720635.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49895.54 Da        Isoelectric Point: 7.3886

>NTDB_id=457034 HU257_RS11325 WP_178306309.1 2505367..2506737(+) (radA) [Enterococcus hirae strain 13152]
MAKKAKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTDRRTRTSLTGEKAKPTKIAEVIPKKEPRVKTKLDELN
RVLGGGVVPGSMILIGGDPGIGKSTLLLQVSQQLADIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
AIEQISPDYVIIDSIQTMAQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEVANPSQIFLEERLEGATGSAIVVAMEGTRPILVEIQALVTPTMYG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECFIGEIGLTG
EIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGGWNPPTGIEVVGVATLAETLKRVFR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=457034 HU257_RS11325 WP_178306309.1 2505367..2506737(+) (radA) [Enterococcus hirae strain 13152]
ATGGCAAAAAAAGCGAAAGTCCAGTTTGTTTGCCAAAATTGTGGTTATGTTTCGCCTAAATTCTTAGGGCGTTGCCCAAA
TTGCGGGAAATGGAACACGATGGTCGAGGAAATCGAGCAAGATACGACTGACCGTAGAACACGAACAAGTTTGACTGGAG
AAAAAGCCAAACCAACGAAAATTGCAGAAGTGATTCCTAAAAAGGAACCACGAGTCAAAACAAAATTAGATGAATTGAAT
CGAGTTTTGGGCGGGGGCGTAGTTCCGGGGTCAATGATTTTGATTGGTGGCGATCCAGGCATCGGTAAATCCACATTACT
TTTACAAGTATCCCAACAATTAGCTGATATCGGCGGGAAAGTATTATATGTTTCTGGAGAAGAAAGTGCAGAACAAATTA
AATTACGTGCCGAGCGTTTGGGCTCAATCAATACAGAATTTTACTTATATGCTGAAACTGATATGAATGAAATTAGCCGT
GCCATCGAACAGATTTCACCAGATTATGTGATCATTGATTCGATTCAAACGATGGCACAACCTGATATTACGAGTGTAGC
AGGGAGTGTCAGTCAAGTGAGAGAAACGACCGCTGAGTTGTTGAAAATAGCGAAAACAAATGGGATTGCGATTTTTATCG
TAGGACATGTAACGAAAGAAGGCTCGATTGCGGGTCCACGTATGCTTGAACATATGGTTGATACTGTTTTGTATTTTGAA
GGAGAACAGCATCATAGTTTTCGTATTTTGAGAGCAGTAAAGAATCGATTTGGTTCAACAAATGAAATTGGTATATTTGA
AATGCATGAACATGGATTAGAAGAAGTTGCGAATCCTTCCCAAATCTTTTTGGAAGAACGATTAGAAGGAGCAACAGGCT
CAGCGATTGTTGTGGCGATGGAAGGTACCCGCCCAATTTTAGTGGAAATCCAAGCACTGGTAACACCAACAATGTATGGA
AATGCTAAACGAACCACTACTGGCTTAGATTTTAACCGAGTATCATTGATCATGGCAGTTCTCGAAAAACGAGCAGGCTT
ACTGTTGCAGAATCAAGATGCTTACTTAAAAGCAGCAGGTGGGGTAAAATTAAATGAACCAGCCATTGATTTAGCAATTG
CTGTGAGTATTGCTTCCAGTTACAAAGAAAAAGGGACACAACCGACAGAATGTTTTATTGGCGAAATCGGGTTGACGGGC
GAAATTCGCCGTGTCAGTCATATTGAACAGCGTGTCAAAGAAGTACAAAAGCTGGGTTTCACAAAAGTTTATTTGCCAAA
GAACAATCTAGGAGGGTGGAATCCTCCGACTGGCATTGAAGTCGTAGGTGTCGCTACCTTGGCGGAAACGTTAAAACGTG
TCTTTCGTTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(71-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis SK321

74.066

99.781

0.739
  radA Streptococcus mitis NCTC 12261

74.066

99.781

0.739
  radA Streptococcus pneumoniae Rx1

73.846

99.781

0.737
  radA Streptococcus pneumoniae D39

73.846

99.781

0.737
  radA Streptococcus pneumoniae R6

73.846

99.781

0.737
  radA Streptococcus pneumoniae TIGR4

73.846

99.781

0.737
  radA Bacillus subtilis subsp. subtilis str. 168

67.406

98.904

0.667