Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   JH656_RS03420 Genome accession   NZ_CP066839
Coordinates   721160..721837 (-) Length   225 a.a.
NCBI ID   WP_000178163.1    Uniprot ID   -
Organism   Escherichia coli strain Ec-RL2-1X     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 716160..726837
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH656_RS03405 (JH656_03350) chiA 717527..720220 (+) 2694 WP_242561970.1 bifunctional chitinase/lysozyme -
  JH656_RS03410 (JH656_03355) bfd 720389..720583 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  JH656_RS03415 (JH656_03360) bfr 720655..721131 (+) 477 WP_000675504.1 bacterioferritin -
  JH656_RS03420 (JH656_03365) pilD 721160..721837 (-) 678 WP_000178163.1 prepilin peptidase GspO Machinery gene
  JH656_RS03425 (JH656_03370) gspM 721837..722298 (-) 462 WP_001295161.1 type II secretion system protein GspM -
  JH656_RS03430 (JH656_03375) gspL 722295..723458 (-) 1164 WP_001115228.1 type II secretion system protein GspL -
  JH656_RS03435 (JH656_03380) gspK 723473..724456 (-) 984 WP_001058521.1 type II secretion system minor pseudopilin GspK -
  JH656_RS03440 (JH656_03385) gspJ 724449..725036 (-) 588 WP_000610634.1 type II secretion system minor pseudopilin GspJ -
  JH656_RS03445 (JH656_03390) gspI 725029..725406 (-) 378 WP_167814358.1 type II secretion system minor pseudopilin GspI -
  JH656_RS03450 (JH656_03395) gspH 725403..725912 (-) 510 WP_001076029.1 type II secretion system minor pseudopilin GspH -
  JH656_RS03455 (JH656_03400) gspG 725920..726357 (-) 438 WP_001202874.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24928.81 Da        Isoelectric Point: 7.9563

>NTDB_id=456594 JH656_RS03420 WP_000178163.1 721160..721837(-) (pilD) [Escherichia coli strain Ec-RL2-1X]
MTMLLPLFILVGFIAGYFVNAIAYHLSPLEDKTALTFRQVLVHFRQKKYAWHDTVPLILCVAAAIACALAPFTPIVTSAL
FLYFCFVLTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQYGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=456594 JH656_RS03420 WP_000178163.1 721160..721837(-) (pilD) [Escherichia coli strain Ec-RL2-1X]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGCCATCGCCTATCACCTCTC
GCCGTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCAGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGTGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATCGTGACTAGCGCACTC
TTTCTCTATTTCTGTTTCGTACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTATGGATTAATTGATTTACATGATGCGGTTTACGGCGCGGTAGCGG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCATGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGGTATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.946

100

0.378