Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoS   Type   Regulator
Locus tag   JH476_RS21385 Genome accession   NZ_CP066836
Coordinates   4453786..4454778 (+) Length   330 a.a.
NCBI ID   WP_000081550.1    Uniprot ID   A0A370V765
Organism   Escherichia coli strain Ec-FL1-1X     
Function   regulation of chitinases (predicted from homology)   
Competence regulation

Genomic Context

Location: 4448786..4459778
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JH476_RS21355 (JH476_21270) ispD 4448847..4449557 (+) 711 WP_000246141.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  JH476_RS21360 (JH476_21275) ispF 4449557..4450036 (+) 480 WP_001219242.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  JH476_RS21365 (JH476_21280) truD 4450033..4451082 (+) 1050 WP_000568953.1 tRNA pseudouridine(13) synthase TruD -
  JH476_RS21370 (JH476_21285) surE 4451063..4451824 (+) 762 WP_001305779.1 5'/3'-nucleotidase SurE -
  JH476_RS21375 (JH476_21290) pcm 4451818..4452444 (+) 627 WP_000254708.1 protein-L-isoaspartate O-methyltransferase -
  JH476_RS21380 (JH476_21295) nlpD 4452584..4453723 (+) 1140 WP_001272592.1 murein hydrolase activator NlpD -
  JH476_RS21385 (JH476_21300) rpoS 4453786..4454778 (+) 993 WP_000081550.1 RNA polymerase sigma factor RpoS Regulator
  JH476_RS21390 (JH476_21305) - 4454899..4455306 (-) 408 WP_001208065.1 MarR family winged helix-turn-helix transcriptional regulator -
  JH476_RS21395 (JH476_21310) - 4455453..4456046 (+) 594 WP_001237176.1 non-oxidative hydroxyarylic acid decarboxylases subunit B -
  JH476_RS21400 (JH476_21315) - 4456046..4457473 (+) 1428 WP_000863184.1 non-oxidative hydroxyarylic acid decarboxylases subunit C -
  JH476_RS21405 (JH476_21320) - 4457484..4457720 (+) 237 WP_000562983.1 non-oxidative hydroxyarylic acid decarboxylases subunit D -
  JH476_RS21410 (JH476_21325) pphB 4457762..4458418 (-) 657 WP_001139307.1 protein-serine/threonine phosphatase -
  JH476_RS21415 (JH476_21330) - 4458443..4459306 (-) 864 WP_001223109.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 330 a.a.        Molecular weight: 37972.84 Da        Isoelectric Point: 4.5597

>NTDB_id=456562 JH476_RS21385 WP_000081550.1 4453786..4454778(+) (rpoS) [Escherichia coli strain Ec-FL1-1X]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide


Download         Length: 993 bp        

>NTDB_id=456562 JH476_RS21385 WP_000081550.1 4453786..4454778(+) (rpoS) [Escherichia coli strain Ec-FL1-1X]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGACGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
GAAAGCCTTAGTTGAAGAGGAACCCAGTGATAACGATTTGGCTGAAGAGGAGCTGTTATCGCAAGGGGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCGCCACTGTTAACGGCCGAAGAAGAAGTTTATTTCGCG
CGTCGCGCACTGCGTGGAGATGTCGCTTCACGTCGCCGGATGATTGAAAGTAACCTGCGTCTGGTCGTGAAGATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGCTGTTGGACCTGATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATCCGCCAGACGATCGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGCGAGTTGTCCCA
TAAACTGGACCACGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

Sigma-70 region 3 (Sigma70_r3)

(174-248)

Sigma-70 region 2 (Sigma70_r2)

(94-163)

Sigma-70 factor, region 1.2 (Sigma70_r1_2)

(56-88)

Sigma-70, region 4 (Sigma70_r4)

(262-315)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A370V765

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoS Vibrio cholerae O1 biovar El Tor strain E7946

73.065

97.879

0.715