Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA/sms   Type   Machinery gene
Locus tag   JG533_RS23465 Genome accession   NZ_CP066759
Coordinates   4689094..4690476 (-) Length   460 a.a.
NCBI ID   WP_001029698.1    Uniprot ID   A0A2T3RLH6
Organism   Escherichia coli O157:H7 strain ECP19-198     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4684094..4695476
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JG533_RS23450 (JG533_23450) ettA 4685866..4687533 (+) 1668 WP_000046749.1 energy-dependent translational throttle protein EttA -
  JG533_RS23455 (JG533_23455) nadS 4687694..4687807 (-) 114 Protein_4661 NadS family protein -
  JG533_RS23460 (JG533_23460) nadR 4687841..4689073 (-) 1233 WP_000093817.1 multifunctional transcriptional regulator/nicotinamide-nucleotide adenylyltransferase/ribosylnicotinamide kinase NadR -
  JG533_RS23465 (JG533_23465) radA/sms 4689094..4690476 (-) 1383 WP_001029698.1 DNA repair protein RadA Machinery gene
  JG533_RS23470 (JG533_23470) serB 4690525..4691493 (-) 969 WP_001132955.1 phosphoserine phosphatase -
  JG533_RS23475 (JG533_23475) ytjB 4691599..4692240 (+) 642 Protein_4665 YtjB family periplasmic protein -
  JG533_RS23480 (JG533_23480) lplA 4692271..4693287 (+) 1017 WP_000105832.1 lipoate--protein ligase LplA -
  JG533_RS23485 (JG533_23485) - 4693319..4693582 (+) 264 WP_000566150.1 helix-turn-helix transcriptional regulator -
  JG533_RS23490 (JG533_23490) deoD 4693743..4694462 (-) 720 WP_000224877.1 purine-nucleoside phosphorylase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 49442.02 Da        Isoelectric Point: 7.1936

>NTDB_id=456331 JG533_RS23465 WP_001029698.1 4689094..4690476(-) (radA/sms) [Escherichia coli O157:H7 strain ECP19-198]
MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPTVARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTG
FKEFDRVLGGGVVPGSAILIGGNPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMVGHVTKDGSLAGPKVLEHCIDCS
VLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQGLREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDH
SMMANPRRVAVGLEQNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQDLVVFGEV
GLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKLSDALSVFDDL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=456331 JG533_RS23465 WP_001029698.1 4689094..4690476(-) (radA/sms) [Escherichia coli O157:H7 strain ECP19-198]
GTGGCAAAAGCTCCAAAACGCGCCTTTGTTTGTAATGAATGCGGGGCCGATTATCCGCGCTGGCAGGGGCAGTGCAGTGC
CTGTCATGCCTGGAACACCATCACCGAGGTGCGTCTTGCTGCGTCGCCTACGGTGGCGCGTAACGAGCGTCTCAGCGGCT
ATGCCGGTAGCGCCGGGGTGGCAAAAGTCCAGAAACTCTCCGATATCAGCCTTGAAGAGCTGCCGCGTTTTTCCACCGGA
TTTAAAGAGTTCGACCGCGTACTAGGCGGCGGCGTGGTGCCAGGAAGTGCCATTCTGATTGGCGGTAACCCTGGTGCGGG
GAAATCCACGCTGCTACTGCAAACGCTGTGCAAACTGGCCCAGCAGATGAAAACGCTGTATGTCACCGGCGAAGAGTCGC
TGCAACAGGTGGCAATGCGCGCTCATCGCCTTGGCCTGCCGACTGACAATCTCAATATGTTGTCGGAAACCAGCATCGAA
CAGATCTGCCTGATTGCCGAAGAAGAGCAACCGAAGCTGATGGTAATTGACTCGATCCAGGTGATGCATATGGCGGATGT
ACAGTCATCGCCTGGTAGCGTGGCGCAGGTGCGTGAAACGGCGGCTTATTTGACACGCTTCGCCAAAACGCGCGGTGTGG
CGATTGTCATGGTGGGGCACGTAACCAAAGATGGTTCGCTGGCTGGCCCGAAAGTGCTGGAACACTGTATCGACTGTTCG
GTGCTTTTAGATGGCGATGCCGACTCCCGTTTTCGCACCTTGCGCAGCCATAAAAACCGCTTCGGCGCGGTGAATGAGCT
GGGCGTCTTCGCGATGACCGAACAGGGGCTGCGTGAAGTCAGCAACCCTTCGGCAATTTTCTTAAGTCGCGGAGATGAAG
TGACCTCCGGCAGCTCCGTGATGGTGGTGTGGGAAGGAACGCGTCCGTTGCTGGTGGAGATTCAGGCGCTGGTCGATCAC
TCGATGATGGCGAATCCGCGCCGCGTGGCAGTGGGGCTGGAACAAAACCGTCTGGCAATCCTGCTGGCTGTCTTGCACCG
TCACGGTGGTCTGCAAATGGCCGATCAGGATGTGTTTGTGAACGTGGTCGGCGGCGTAAAGGTAACCGAAACCAGCGCCG
ACTTAGCGTTACTGCTGGCGATGGTTTCCAGCCTGCGTGACAGACCGCTACCGCAGGATCTGGTGGTGTTTGGTGAAGTC
GGGCTGGCAGGGGAGATCCGCCCGGTGCCCAGCGGTCAGGAACGAATCTCTGAAGCGGCGAAACACGGTTTTCGCCGGGC
GATTGTTCCGGCGGCTAACGTACCGAAAAAAGCGCCGGAAGGGATGCAGATTTTTGGCGTTAAAAAACTCTCCGACGCGC
TTAGCGTGTTCGACGACTTATAA

Domains

Predicted by InterProScan.

AAA domain (AAA_25)

(89-225)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(348-433)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2T3RLH6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA/sms Bacillus subtilis subsp. subtilis str. 168

46.827

99.348

0.465
  radA Streptococcus mitis SK321

43.86

99.13

0.435
  radA Streptococcus pneumoniae D39

43.421

99.13

0.43
  radA Streptococcus pneumoniae TIGR4

43.421

99.13

0.43
  radA Streptococcus mitis NCTC 12261

43.421

99.13

0.43
  radA Streptococcus pneumoniae R6

43.421

99.13

0.43
  radA Streptococcus pneumoniae Rx1

43.421

99.13

0.43