Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   I5P61_RS02320 Genome accession   NZ_CP066743
Coordinates   490447..491124 (-) Length   225 a.a.
NCBI ID   WP_000178177.1    Uniprot ID   -
Organism   Escherichia coli strain UPEC 536     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 485447..496124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5P61_RS02305 (I5P61_00456) chiA 486805..489507 (+) 2703 WP_000773146.1 bifunctional chitinase/lysozyme -
  I5P61_RS02310 (I5P61_00457) bfd 489676..489870 (+) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  I5P61_RS02315 (I5P61_00458) bfr 489942..490418 (+) 477 WP_000675504.1 bacterioferritin -
  I5P61_RS02320 (I5P61_00459) pilD 490447..491124 (-) 678 WP_000178177.1 prepilin peptidase GspO Machinery gene
  I5P61_RS02325 (I5P61_00460) istB 491222..492004 (-) 783 WP_001317493.1 IS21-like element IS100kyp family helper ATPase IstB -
  I5P61_RS02330 (I5P61_00461) istA 492001..493023 (-) 1023 WP_001298025.1 IS21-like element IS100 family transposase -
  I5P61_RS02335 (I5P61_00462) - 493050..493544 (-) 495 WP_000597912.1 type II secretion system protein M -
  I5P61_RS02340 (I5P61_00463) gspL 493541..494704 (-) 1164 WP_001335091.1 type II secretion system protein GspL -
  I5P61_RS02345 (I5P61_00464) gspK 494719..495702 (-) 984 WP_001058509.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24900.77 Da        Isoelectric Point: 7.6364

>NTDB_id=455867 I5P61_RS02320 WP_000178177.1 490447..491124(-) (pilD) [Escherichia coli strain UPEC 536]
MTMLLPLFILVGFIASYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=455867 I5P61_RS02320 WP_000178177.1 490447..491124(-) (pilD) [Escherichia coli strain UPEC 536]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAAGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

32.95

100

0.382