Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   I5P57_RS01920 Genome accession   NZ_CP066740
Coordinates   417615..418292 (-) Length   225 a.a.
NCBI ID   WP_000178175.1    Uniprot ID   A0A0H2Z3H2
Organism   Escherichia coli strain R12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 412615..423292
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5P57_RS01905 (I5P57_00379) chiA 413973..416675 (+) 2703 WP_001765222.1 bifunctional chitinase/lysozyme -
  I5P57_RS01910 (I5P57_00380) bfd 416844..417038 (+) 195 WP_001765223.1 bacterioferritin-associated ferredoxin -
  I5P57_RS01915 (I5P57_00381) bfr 417110..417586 (+) 477 WP_000675504.1 bacterioferritin -
  I5P57_RS01920 (I5P57_00382) pilD 417615..418292 (-) 678 WP_000178175.1 prepilin peptidase GspO Machinery gene
  I5P57_RS01925 (I5P57_00383) gspM 418292..418753 (-) 462 WP_001298207.1 type II secretion system protein GspM -
  I5P57_RS01930 (I5P57_00384) gspL 418750..419913 (-) 1164 WP_001765224.1 type II secretion system protein GspL -
  I5P57_RS01935 (I5P57_00385) gspK 419928..420911 (-) 984 WP_001765225.1 type II secretion system minor pseudopilin GspK -
  I5P57_RS01940 (I5P57_00386) gspJ 420904..421491 (-) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  I5P57_RS01945 (I5P57_00387) gspI 421484..421861 (-) 378 WP_001041748.1 type II secretion system minor pseudopilin GspI -
  I5P57_RS01950 (I5P57_00388) gspH 421858..422367 (-) 510 WP_001076035.1 type II secretion system minor pseudopilin GspH -
  I5P57_RS01955 (I5P57_00389) gspG 422375..422812 (-) 438 WP_001765226.1 type II secretion system major pseudopilin GspG -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24870.75 Da        Isoelectric Point: 7.6364

>NTDB_id=455782 I5P57_RS01920 WP_000178175.1 417615..418292(-) (pilD) [Escherichia coli strain R12]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=455782 I5P57_RS01920 WP_000178175.1 417615..418292(-) (pilD) [Escherichia coli strain R12]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387