Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   JFJ84_RS08155 Genome accession   NZ_CP066739
Coordinates   1724982..1726325 (-) Length   447 a.a.
NCBI ID   WP_012341565.1    Uniprot ID   -
Organism   Histophilus somni strain ASc-MMNZ-VFA-069     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1719982..1731325
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JFJ84_RS08140 (JFJ84_08135) typA 1720796..1722646 (+) 1851 WP_012341568.1 translational GTPase TypA -
  JFJ84_RS08145 (JFJ84_08140) rpsT 1722708..1722971 (-) 264 WP_011608572.1 30S ribosomal protein S20 -
  JFJ84_RS08150 (JFJ84_08145) murJ 1723249..1724820 (+) 1572 WP_012341567.1 murein biosynthesis integral membrane protein MurJ -
  JFJ84_RS08155 (JFJ84_08150) pilA 1724982..1726325 (-) 1344 WP_012341565.1 signal recognition particle-docking protein FtsY Machinery gene
  JFJ84_RS08160 (JFJ84_08155) rsmD 1726389..1726991 (+) 603 WP_012341564.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  JFJ84_RS08165 (JFJ84_08160) dprA 1726974..1728080 (-) 1107 WP_012341563.1 DNA-processing protein DprA Machinery gene
  JFJ84_RS08170 (JFJ84_08165) tsaD 1728315..1729343 (+) 1029 WP_012341562.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD -
  JFJ84_RS08175 (JFJ84_08170) - 1729405..1729992 (+) 588 WP_012341561.1 thymidine kinase -
  JFJ84_RS08180 (JFJ84_08175) - 1730153..1730824 (+) 672 WP_012341560.1 trimeric intracellular cation channel family protein -

Sequence

Protein


Download         Length: 447 a.a.        Molecular weight: 49742.15 Da        Isoelectric Point: 4.4499

>NTDB_id=455764 JFJ84_RS08155 WP_012341565.1 1724982..1726325(-) (pilA) [Histophilus somni strain ASc-MMNZ-VFA-069]
MSEEKKKGRFWSWLGLGQKTEDSISEQQPEQQSGQQESVASEQESTSNIEQVIKEPEHIDDTSSDLVLSAHLQEQEVKEY
SLHERELSQNTVIEIESAVEKTDIFIEEDTTYQAINKEQREETTITSVKEVIPQEQEKPAENGFFSRLVKGLLKTKQNIG
IGFRSLFLGKKIDDELFEELEEQLLIADIGVPTTTKIINNLIQHASRKQLQEADLLYQQLKVELADILKPVEQPLQLDNS
KKPYVILMVGVNGVGKTTTIGKLANQFQRQGKSVMLAAGDTFRAAAVEQLQVWGERNQIPVIAQSTGSDSASVIFDAMQS
AAARNIDVLIADTAGRLQNKNNLMDELKKIVRVIKKCDETAPHEIMLTLDSGTGQNAISQAKLFNEAVGLTGISLTKLDG
TAKGGVIFAIADQFNLPIRYIGVGEKIDDLRPFQADEFIDALFVQEE

Nucleotide


Download         Length: 1344 bp        

>NTDB_id=455764 JFJ84_RS08155 WP_012341565.1 1724982..1726325(-) (pilA) [Histophilus somni strain ASc-MMNZ-VFA-069]
ATGTCAGAAGAAAAGAAAAAAGGTAGATTTTGGTCTTGGTTAGGATTGGGGCAGAAAACAGAAGATTCGATATCAGAACA
GCAACCGGAGCAACAATCAGGACAACAGGAAAGTGTTGCAAGCGAGCAAGAATCTACTTCGAATATAGAGCAGGTTATAA
AAGAACCAGAGCATATTGATGACACTTCATCAGACTTGGTTTTATCCGCTCATTTACAGGAGCAAGAGGTAAAAGAATAC
TCACTTCATGAACGGGAACTGTCTCAAAATACTGTAATAGAAATAGAAAGTGCGGTTGAAAAAACAGATATTTTTATTGA
AGAGGATACAACATATCAGGCTATCAATAAGGAGCAGCGAGAGGAAACAACGATTACATCGGTAAAAGAAGTAATACCGC
AAGAACAAGAAAAACCGGCTGAAAATGGTTTTTTTAGTCGTTTAGTCAAAGGATTATTAAAAACCAAGCAAAATATCGGT
ATCGGTTTCCGCAGTTTGTTTTTGGGCAAGAAAATTGATGACGAGTTATTTGAGGAGTTAGAAGAACAACTATTGATCGC
TGATATCGGTGTGCCAACTACGACAAAAATCATCAATAATTTAATTCAGCATGCAAGCCGTAAACAATTGCAAGAGGCAG
ATTTACTTTATCAACAACTTAAAGTGGAATTAGCCGATATTTTAAAACCGGTCGAGCAACCTTTACAACTTGATAACAGT
AAAAAACCTTATGTTATCTTGATGGTTGGTGTAAATGGTGTGGGTAAAACCACTACAATCGGCAAATTGGCTAATCAATT
CCAAAGGCAAGGCAAATCCGTTATGTTGGCGGCAGGAGATACGTTCCGTGCTGCGGCTGTTGAGCAGCTTCAAGTTTGGG
GAGAACGTAACCAGATTCCTGTTATTGCACAAAGTACAGGGTCAGATTCTGCTTCTGTCATTTTTGATGCTATGCAATCG
GCAGCGGCACGTAATATTGATGTACTTATTGCGGATACGGCTGGGCGTTTGCAGAATAAAAATAACCTGATGGATGAGTT
GAAAAAAATTGTTCGTGTGATAAAAAAATGTGATGAAACAGCACCGCATGAAATCATGCTAACTCTTGATAGTGGAACGG
GACAAAATGCAATTAGTCAAGCGAAATTGTTCAATGAAGCGGTAGGATTAACGGGTATTAGTCTAACCAAGTTGGATGGA
ACAGCAAAAGGCGGTGTTATTTTTGCGATTGCAGATCAATTTAATTTACCTATTCGCTATATTGGTGTCGGTGAGAAAAT
CGATGATTTACGCCCATTCCAAGCAGATGAATTTATTGACGCACTTTTTGTACAAGAAGAGTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(244-443)

SRP54-type protein, helical bundle domain (SRP54_N)

(163-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

50.151

74.049

0.371