Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   JFL50_RS08150 Genome accession   NZ_CP066559
Coordinates   1724999..1726342 (-) Length   447 a.a.
NCBI ID   WP_198973426.1    Uniprot ID   -
Organism   Histophilus somni strain ASc-MMNZ-VFA-071     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1719999..1731342
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JFL50_RS08135 (JFL50_08135) typA 1720813..1722663 (+) 1851 WP_012341568.1 translational GTPase TypA -
  JFL50_RS08140 (JFL50_08140) rpsT 1722725..1722988 (-) 264 WP_011608572.1 30S ribosomal protein S20 -
  JFL50_RS08145 (JFL50_08145) murJ 1723266..1724837 (+) 1572 WP_132994704.1 murein biosynthesis integral membrane protein MurJ -
  JFL50_RS08150 (JFL50_08150) pilA 1724999..1726342 (-) 1344 WP_198973426.1 signal recognition particle-docking protein FtsY Machinery gene
  JFL50_RS08155 (JFL50_08155) rsmD 1726406..1727008 (+) 603 WP_012341564.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  JFL50_RS08160 (JFL50_08160) dprA 1726991..1728097 (-) 1107 WP_132994706.1 DNA-processing protein DprA Machinery gene
  JFL50_RS08165 (JFL50_08165) tsaD 1728323..1729351 (+) 1029 WP_132994707.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD -
  JFL50_RS08170 (JFL50_08170) - 1729413..1730000 (+) 588 WP_011608566.1 thymidine kinase -
  JFL50_RS08175 (JFL50_08175) - 1730161..1730832 (+) 672 WP_132994708.1 trimeric intracellular cation channel family protein -

Sequence

Protein


Download         Length: 447 a.a.        Molecular weight: 49863.34 Da        Isoelectric Point: 4.4501

>NTDB_id=455325 JFL50_RS08150 WP_198973426.1 1724999..1726342(-) (pilA) [Histophilus somni strain ASc-MMNZ-VFA-071]
MSEEKKKGRFWSWLGLGQKTEDPISEQQPEQQSGQQESVAREQESTSNIEQVIKEPEHIDDTSSDLVLSAHLQEQEVKEY
SLYERELSQNTVIETESAVEKTDIFIEEDTTYQAINKEQREETTITSVKEVIPQEQEKPAENGFFSRLVKGLLKTKQNIG
IGFRSLFLGKKIDDELFEELEEQLLIADIGVPTTTKIINNLIQHASRKQLQEADLLYQQLKVELADILKPVEQPLQLDNS
KKPYVILMVGVNGVGKTTTIGKLANQFQRQGKSVMLAAGDTFRAAAVEQLQVWGERNQIPVIAQSTGSDSASVIFDAMQS
AVARNIDVLIADTAGRLQNKNNLMDELKKIVRVIKKCDETAPHEIMLTLDSGTGQNAISQAKLFNEAVGLTGISLTKLDG
TAKGGVIFAIADQFNLPIRYIGVGEKIDDLRPFQADEFIDALFVQEE

Nucleotide


Download         Length: 1344 bp        

>NTDB_id=455325 JFL50_RS08150 WP_198973426.1 1724999..1726342(-) (pilA) [Histophilus somni strain ASc-MMNZ-VFA-071]
ATGTCAGAAGAAAAGAAAAAAGGTAGATTTTGGTCTTGGTTAGGATTGGGGCAGAAAACAGAAGATCCGATATCAGAACA
GCAACCGGAGCAACAATCAGGACAACAGGAAAGTGTTGCAAGGGAGCAAGAATCTACTTCGAATATAGAGCAGGTTATAA
AAGAACCAGAGCATATTGATGACACTTCATCAGACTTGGTTTTATCCGCTCATTTACAGGAGCAAGAGGTAAAAGAATAC
TCACTTTATGAACGGGAACTGTCTCAAAATACTGTAATAGAAACAGAAAGTGCGGTTGAAAAAACAGATATTTTTATTGA
AGAGGATACAACATATCAGGCTATCAATAAGGAGCAGCGAGAGGAAACAACGATTACATCGGTTAAAGAAGTAATACCGC
AAGAACAAGAAAAACCGGCTGAAAATGGTTTTTTCAGTCGTTTAGTCAAAGGATTATTAAAAACCAAGCAAAATATCGGT
ATCGGTTTCCGCAGTTTGTTTTTGGGCAAGAAAATTGATGACGAGTTATTTGAGGAGTTAGAAGAACAACTATTGATCGC
TGATATCGGTGTGCCAACTACGACAAAAATCATCAATAATTTAATTCAGCATGCAAGCCGTAAACAATTGCAAGAGGCAG
ATTTACTTTATCAACAACTTAAAGTGGAATTAGCCGATATTTTAAAACCGGTCGAGCAACCTTTACAACTTGATAACAGT
AAAAAACCTTATGTTATCTTGATGGTTGGTGTAAATGGTGTGGGTAAAACCACTACAATCGGCAAATTGGCTAATCAATT
CCAAAGGCAAGGCAAATCCGTTATGTTGGCGGCAGGAGATACGTTCCGTGCTGCGGCTGTTGAGCAGCTTCAAGTTTGGG
GAGAACGTAACCAGATTCCTGTTATTGCACAAAGTACAGGTTCAGATTCTGCTTCTGTCATTTTTGATGCTATGCAATCG
GCAGTGGCACGTAATATTGATGTGCTTATTGCGGATACGGCTGGGCGTTTGCAGAATAAAAATAACCTGATGGATGAGTT
GAAAAAAATTGTTCGTGTGATAAAAAAATGTGATGAAACAGCACCGCATGAAATCATGCTAACTCTTGATAGTGGAACGG
GACAAAATGCAATTAGTCAAGCGAAATTGTTCAATGAAGCGGTAGGATTAACGGGTATTAGTCTAACCAAGTTGGATGGA
ACAGCAAAAGGCGGTGTTATTTTTGCGATTGCAGATCAATTTAATTTACCTATTCGCTATATTGGTGTCGGTGAGAAAAT
CGATGATTTACGCCCATTCCAAGCAGATGAATTTATTGACGCACTTTTTGTACAAGAAGAGTAA

Domains

Predicted by InterProScan.

SRP54-type protein, helical bundle domain (SRP54_N)

(163-224)

SRP54-type protein, GTPase domain (SRP54)

(244-443)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

50.151

74.049

0.371