Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HUI97_RS18735 Genome accession   NZ_CP054855
Coordinates   4098496..4099935 (-) Length   479 a.a.
NCBI ID   WP_005336331.1    Uniprot ID   A0A0T6N7F3
Organism   Aeromonas veronii strain 1708-29120     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4093496..4104935
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUI97_RS18705 (HUI97_18705) dagF 4093764..4094504 (+) 741 WP_206900654.1 2-dehydro-3-deoxy-phosphogluconate aldolase -
  HUI97_RS18710 (HUI97_18710) - 4094612..4096516 (+) 1905 WP_206900655.1 BglG family transcription antiterminator -
  HUI97_RS18715 (HUI97_18715) npr 4096534..4096806 (-) 273 WP_005336318.1 PTS phosphocarrier protein NPr -
  HUI97_RS18720 (HUI97_18720) rapZ 4096827..4097693 (-) 867 WP_206900656.1 RNase adapter RapZ -
  HUI97_RS18725 (HUI97_18725) ptsN 4097739..4098185 (-) 447 WP_005336322.1 PTS IIA-like nitrogen regulatory protein PtsN -
  HUI97_RS18730 (HUI97_18730) hpf 4098188..4098475 (-) 288 WP_005336323.1 ribosome hibernation promoting factor -
  HUI97_RS18735 (HUI97_18735) rpoN/rpoN1 4098496..4099935 (-) 1440 WP_005336331.1 RNA polymerase factor sigma-54 Machinery gene
  HUI97_RS18740 (HUI97_18740) lptB 4100001..4100726 (-) 726 WP_005336333.1 LPS export ABC transporter ATP-binding protein -
  HUI97_RS18745 (HUI97_18745) lptA 4100730..4101257 (-) 528 WP_040068817.1 lipopolysaccharide transport periplasmic protein LptA -
  HUI97_RS18750 (HUI97_18750) lptC 4101244..4101804 (-) 561 WP_058057520.1 LPS export ABC transporter periplasmic protein LptC -
  HUI97_RS18755 (HUI97_18755) kdsC 4101801..4102355 (-) 555 WP_040068814.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  HUI97_RS18760 (HUI97_18760) - 4102355..4103350 (-) 996 WP_005336342.1 KpsF/GutQ family sugar-phosphate isomerase -
  HUI97_RS18765 (HUI97_18765) mlaF 4103558..4104358 (+) 801 WP_206900657.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53701.64 Da        Isoelectric Point: 4.3442

>NTDB_id=455002 HUI97_RS18735 WP_005336331.1 4098496..4099935(-) (rpoN/rpoN1) [Aeromonas veronii strain 1708-29120]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALENNPLLEQEESEAQETSPEAPVDASQLDSSDAMAQEQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVALED
IQAAVTSDDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLDQYDPQTPWLNEAKEVILEHMELLGNRDYRTLARK
TKLKESDLKEVLDLIQLLEPRPGNGIIQSDSQYVIPDVSVVKKGSKWVVELNPDSAPRIRVNETYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=455002 HUI97_RS18735 WP_005336331.1 4098496..4099935(-) (rpoN/rpoN1) [Aeromonas veronii strain 1708-29120]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAAAACAACCCTCTGTTGGAGCAGGAAGAGAGCGAGG
CGCAAGAGACCAGTCCGGAAGCGCCGGTCGATGCCAGCCAGCTGGATTCCAGCGATGCCATGGCTCAGGAGCAGATGCCG
GACGAGTTGCCGGTGGATACCACCTGGGACGAGGTTTACTCCGCCGGCACCGCCCAGAGCGCGGGCCCCATCCACGATGG
GGAAGATAGCGTCTATCAGGGAGAGACCACCGAAAACCTGCAGGATTATCTGCTGTGGCAGATGCGGCTCACGCCGTTCA
GCGATGTGGATGCCGCCATCGCGCTCGCCATCATCGATGCCATCGACGAATCAGGTTATCTGACCGTTGCGCTGGAGGAT
ATCCAGGCTGCCGTCACCAGCGATGACGTCGAAGTGGAACTCGATGAGGTCGAGGCGGTACTCAAGCGGGTGCAGCACTT
CGATCCGGTCGGCATTGCTGCCCGTTCTGTGCAGGAGTGTTTGCTGATCCAGCTGGATCAGTATGACCCGCAGACCCCCT
GGCTCAACGAGGCCAAGGAGGTGATCCTCGAGCACATGGAGCTGCTCGGCAACCGGGATTACCGTACCCTCGCCCGCAAG
ACAAAACTCAAAGAGAGCGACCTCAAGGAGGTGCTGGATCTGATCCAGCTGCTGGAGCCGCGTCCCGGCAACGGCATCAT
CCAGAGCGACTCCCAGTACGTGATCCCCGACGTCTCCGTGGTCAAGAAGGGCAGCAAGTGGGTGGTCGAGCTCAATCCCG
ACTCCGCTCCCCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGCAACGCTCGCAGCAGCACCGACACCCAGTTT
ATCCGCTCCCATTTGCAGGAAGCCAAGTGGTTCATCAAGAGCCTGGAAAGTCGCAATGACACCCTGCTGAAAGTGGCCAG
TTGCATTGTCGAGCAGCAACAGGGCTTTTTCGAGTATGGTGAAGAGGCGATGAAGCCCATGGTGCTCAATGACATCGCCG
AAGCGGTAGAGATGCATGAGTCGACCATCTCCCGGGTGACGACCCAGAAGTACATGCATACCCCTCGTGGTATCTTCGAA
TTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCAGAGAATCCTGCCAAGCCGTTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCTAAGTATCGCGAATCCTTGTTTATCCCACCTTCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0T6N7F3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.988

100

0.491