Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HUK68_RS01015 Genome accession   NZ_CP054840
Coordinates   218390..219964 (+) Length   524 a.a.
NCBI ID   WP_175502515.1    Uniprot ID   -
Organism   Comamonas antarctica strain 16-35-5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 213390..224964
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUK68_RS00990 (HUK68_00990) argS 213670..215367 (+) 1698 WP_175502510.1 arginine--tRNA ligase -
  HUK68_RS00995 (HUK68_00995) - 215380..216060 (+) 681 WP_175502511.1 SPOR domain-containing protein -
  HUK68_RS01000 (HUK68_01000) - 216116..216769 (+) 654 WP_175502512.1 thiol:disulfide interchange protein DsbA/DsbL -
  HUK68_RS01005 (HUK68_01005) lptA 216946..217569 (+) 624 WP_175502513.1 lipopolysaccharide transport periplasmic protein LptA -
  HUK68_RS01010 (HUK68_01010) lptB 217581..218348 (+) 768 WP_244146224.1 LPS export ABC transporter ATP-binding protein -
  HUK68_RS01015 (HUK68_01015) rpoN/rpoN1 218390..219964 (+) 1575 WP_175502515.1 RNA polymerase factor sigma-54 Machinery gene
  HUK68_RS01020 (HUK68_01020) - 220037..221323 (+) 1287 WP_175502516.1 THUMP domain-containing protein -
  HUK68_RS01025 (HUK68_01025) - 221320..221838 (+) 519 WP_175502517.1 PIN domain-containing protein -
  HUK68_RS01030 (HUK68_01030) - 221951..222802 (+) 852 WP_175502518.1 YecA family protein -
  HUK68_RS01035 (HUK68_01035) - 222889..224370 (+) 1482 WP_175502519.1 SulP family inorganic anion transporter -
  HUK68_RS01040 (HUK68_01040) - 224469..224942 (-) 474 WP_175502520.1 CinA family protein -

Sequence

Protein


Download         Length: 524 a.a.        Molecular weight: 57971.97 Da        Isoelectric Point: 4.8638

>NTDB_id=454832 HUK68_RS01015 WP_175502515.1 218390..219964(+) (rpoN/rpoN1) [Comamonas antarctica strain 16-35-5]
MKPGLSLRVSQHLALTPQLQQSIWLLQLSTLELSQEVEHMLDDNPFLERTTEEAEREEFGVAQADLPAQRDDYADDAQHA
TGSASSDTATSSDTESGDVTAPADAAPDWEGDGSADLSPDDSEWGGEARARHAEDDTEAQDLAHGHESLTDHLHRQALSL
RLGEVDRSALRFLIESLNEHGYLEDPLEELAAGLAGADALEEQEELVHHFTVALRLLQSLEPVGVGARDLAECLTLQLRH
LAARDDAQVDADTVQTALRICEQPMDLLARRDVRRLMQACGASDERIRAAMALIARLEPRPGRRFADVERNLVVPDVMVR
KLARSSGDGQPQFSVQLNPDVMPRLRVHDIYANALRGHKGSEGHAGLQQRLQEARWFIKNIQQRFDTILRVSQAIVQRQK
NFFTHGELAMRPLVLRDIADELGLHESTISRVTTAKYMSTPMGTYELKYFFGSGLGTETGGNASSTAVRALIKQFVAAEN
PLKPLSDSQLAEMLKEQGIECARRTVAKYRDVLKIAPANLRKSL

Nucleotide


Download         Length: 1575 bp        

>NTDB_id=454832 HUK68_RS01015 WP_175502515.1 218390..219964(+) (rpoN/rpoN1) [Comamonas antarctica strain 16-35-5]
TTGAAACCGGGTCTGTCGCTACGCGTATCGCAACATCTGGCCCTCACACCGCAGTTGCAGCAATCGATCTGGCTGCTCCA
GCTCTCGACGCTGGAACTGAGCCAGGAGGTCGAGCACATGCTCGACGACAACCCGTTCCTGGAGCGCACCACCGAGGAAG
CCGAGCGCGAGGAGTTTGGCGTGGCACAGGCCGATCTGCCCGCACAGCGCGACGATTACGCCGATGACGCGCAGCACGCG
ACGGGTTCGGCCAGCAGCGACACGGCCACCAGCAGCGATACCGAGAGCGGCGACGTCACGGCGCCGGCCGATGCCGCGCC
CGATTGGGAAGGGGATGGCAGCGCCGACCTCTCGCCCGACGACAGCGAATGGGGCGGCGAGGCGCGTGCGCGCCATGCCG
AGGACGACACCGAAGCCCAGGATCTGGCGCACGGCCATGAGTCGCTGACCGACCACCTGCACCGCCAGGCACTGTCGCTG
CGCCTGGGCGAGGTCGATCGCTCGGCGCTGCGCTTTCTCATTGAATCGCTCAACGAACATGGCTATCTCGAGGACCCGCT
CGAGGAACTGGCCGCGGGCCTGGCCGGTGCCGATGCGCTCGAGGAGCAGGAAGAACTGGTGCACCACTTCACGGTGGCGC
TGCGCCTGCTGCAGTCGCTCGAGCCGGTGGGCGTGGGCGCGCGCGACCTGGCCGAATGCCTGACGCTGCAGCTCAGGCAC
CTCGCCGCGCGCGATGACGCGCAGGTGGACGCGGATACCGTGCAGACCGCGCTGCGCATCTGCGAGCAGCCCATGGACCT
GCTGGCGCGCCGCGATGTGCGCCGGCTGATGCAGGCCTGCGGCGCGAGCGACGAGCGCATCCGCGCGGCCATGGCGCTGA
TTGCGCGGCTCGAACCGCGTCCCGGCCGGCGCTTTGCCGATGTCGAGCGCAACCTGGTCGTGCCCGATGTGATGGTGCGC
AAGCTGGCGCGCAGCAGTGGCGACGGCCAGCCGCAGTTCAGCGTGCAGCTCAACCCCGACGTCATGCCGCGGCTGCGCGT
GCATGACATCTATGCCAACGCGCTGCGCGGCCACAAGGGCAGCGAAGGACATGCGGGCCTGCAGCAGCGCCTGCAGGAGG
CGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTCTCCCAGGCCATCGTGCAGCGGCAGAAG
AACTTCTTCACCCATGGCGAGCTGGCGATGCGCCCGCTGGTGCTGCGCGACATCGCCGACGAACTGGGCCTGCACGAGTC
GACCATCAGCCGCGTGACCACGGCCAAGTACATGTCCACGCCGATGGGCACCTATGAACTCAAGTACTTCTTTGGTTCAG
GCCTGGGCACCGAGACCGGGGGCAACGCCTCGAGCACGGCGGTGCGCGCGCTGATCAAGCAGTTCGTCGCGGCCGAGAAT
CCGCTCAAGCCGTTGTCGGACAGCCAGCTGGCGGAGATGCTCAAGGAGCAGGGCATCGAGTGCGCGCGCCGTACCGTGGC
CAAGTACCGCGATGTGCTCAAGATCGCGCCCGCCAATCTGCGCAAGTCGCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(140-351)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(367-522)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

51.992

100

0.523