Detailed information NTG0045480

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: A7A1_RS19145
• Genome accession: NC_019896
• Coordinates: 3736791..3737912 (-)
• Length: 373 a.a.
• NCBI ID: WP_003246478.1
• Uniprot ID: O34327
• Organism: Bacillus subtilis subsp. subtilis str. BSP1
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3731791..3742912

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A7A1_RS19125 (A7A1_1912)	znuC	3732299..3732994 (-)	696	WP_014475714.1	zinc ABC transporter ATP-binding protein ZnuC	-
A7A1_RS19130 (A7A1_1913)	znuA	3733049..3734008 (-)	960	WP_003234733.1	metal ABC transporter substrate-binding protein	-
A7A1_RS19135 (A7A1_1914)	ycdG	3734197..3735882 (-)	1686	WP_015252887.1	alpha-glucosidase	-
A7A1_RS19140 (A7A1_1915)	ycdF	3735907..3736683 (-)	777	WP_003234739.1	SDR family oxidoreductase	-
A7A1_RS19145 (A7A1_1916)	rapF	3736791..3737912 (-)	1122	WP_003246478.1	response regulator aspartate phosphatase RapJ	Regulator
A7A1_RS19150 (A7A1_1917)	cwlK	3738035..3738538 (+)	504	WP_015252888.1	peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK	-
A7A1_RS19155 (A7A1_1918)	ycdC	3738546..3739907 (-)	1362	WP_015252889.1	YcdB/YcdC domain-containing protein	-
A7A1_RS19160 (A7A1_1919)	ycdB	3740094..3741515 (-)	1422	WP_015252890.1	YcdB/YcdC domain-containing protein	-
A7A1_RS21715	-	3741689..3741820 (+)	132	WP_003246334.1	hypothetical protein	-
A7A1_RS19165 (A7A1_1920)	ycdA	3741843..3742907 (+)	1065	WP_015252891.1	DUF4352 domain-containing protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44414.31 Da
• Isoelectric Point: 4.8371

>NTDB_id=45480 A7A1_RS19145 WP_003246478.1 3736791..3737912(-) (rapF) [Bacillus subtilis subsp. subtilis str. BSP1]
MRAKIPSEEVAVKLNEWYKLIRAFEADQAEALKQEIEYDLEDMEENQDLLLYFSLMEFRHRIMLDKLMPVKDSDTKPPFS
DMLNEIESNQQKLTGLLEYYFYYFRGMYEFKQKNFILAIDHYKHAEEKLEYVEDEIEKAEFLFKVAEVYYHIKQTYFSMN
YASQALDIYTKYELYGRRRVQCEFIIAGNLTDVYHHEKALTHLCSALEHARQLEEAYMIAAAYYNVGHCKYSLGDYKEAE
GYFKTAAAIFEEHNFQQAVQAVFSLTHIYCKEGKYDKAVEAYDRGIKSAAEWEDDMYLTKFRLIHELYLGSGDLNVLTEC
FDLLESRQLLADAEDLLHDTAERFNQLEHYESAAFFYRRLMNIKKKLAEQRFQ

Nucleotide

• Length: 1122 bp

>NTDB_id=45480 A7A1_RS19145 WP_003246478.1 3736791..3737912(-) (rapF) [Bacillus subtilis subsp. subtilis str. BSP1]
ATGAGAGCAAAGATTCCATCAGAAGAAGTAGCAGTAAAGCTGAATGAATGGTACAAGCTCATTCGCGCATTTGAAGCAGA
TCAAGCAGAAGCGTTAAAGCAGGAGATTGAATACGATTTAGAAGATATGGAAGAGAACCAGGACTTGCTTTTATATTTTT
CTTTGATGGAATTCCGGCATCGAATCATGCTTGATAAGCTGATGCCGGTGAAAGACAGCGACACCAAGCCTCCGTTCTCT
GATATGCTGAACGAAATTGAAAGCAATCAGCAAAAACTCACGGGCTTACTGGAATACTACTTTTATTATTTCAGAGGAAT
GTACGAATTCAAGCAGAAAAATTTCATATTAGCGATTGACCATTATAAACATGCCGAGGAAAAGCTCGAGTATGTCGAGG
ATGAGATCGAAAAAGCTGAGTTTCTTTTTAAAGTCGCAGAAGTGTATTATCACATCAAACAAACGTATTTTTCCATGAAT
TATGCGAGCCAGGCGCTTGATATCTATACGAAATATGAACTGTACGGTCGCCGCCGGGTTCAGTGTGAATTTATCATCGC
CGGAAATTTGACCGATGTGTATCATCATGAAAAAGCACTGACCCACTTGTGCAGCGCTTTAGAACATGCCAGGCAGCTTG
AAGAAGCGTACATGATCGCCGCTGCCTATTATAATGTCGGACACTGTAAGTACAGTCTGGGTGATTACAAAGAAGCGGAG
GGCTATTTCAAAACAGCCGCCGCCATTTTTGAGGAGCACAACTTTCAGCAAGCTGTTCAAGCTGTATTTTCACTGACCCA
TATCTACTGTAAAGAGGGAAAATACGACAAGGCAGTAGAGGCGTATGACCGCGGGATAAAGAGCGCGGCTGAATGGGAGG
ATGACATGTACTTGACGAAATTCCGCCTCATTCATGAGCTGTATCTTGGAAGCGGCGACCTGAACGTGCTTACAGAATGC
TTTGACCTGCTGGAATCCAGGCAGCTTCTTGCTGACGCCGAAGATCTGCTGCATGATACAGCGGAACGTTTCAATCAGCT
GGAACATTACGAGTCAGCCGCTTTCTTTTACCGCAGGCTGATGAATATTAAAAAGAAGCTTGCCGAGCAGCGTTTTCAAT
AG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (196-252)

3D structure

• PDB: 4GYO

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.391	98.66	0.418
rapC	Bacillus subtilis subsp. subtilis str. 168	41.257	98.123	0.405