Detailed information NTG0045447

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: I33_RS18095
• Genome accession: NC_017195
• Coordinates: 3649219..3650364 (+)
• Length: 381 a.a.
• NCBI ID: WP_014478296.1
• Uniprot ID: -
• Organism: Bacillus subtilis subsp. subtilis str. RO-NN-1
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3644219..3655364

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I33_RS18070 (I33_3887)	albF	3644234..3645514 (+)	1281	WP_014478291.1	pitrilysin family protein	-
I33_RS18075 (I33_3888)	albG	3645511..3646212 (+)	702	WP_014478292.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
I33_RS18080 (I33_3889)	ywhL	3646218..3647594 (-)	1377	WP_014478293.1	YncE family protein	-
I33_RS18085 (I33_3890)	ywhK	3647634..3648989 (-)	1356	WP_014478294.1	YncE family protein	-
I33_RS21540 (I33_3891)	-	3648986..3649216 (-)	231	WP_041519575.1	hypothetical protein	-
I33_RS18095 (I33_3892)	rapF	3649219..3650364 (+)	1146	WP_014478296.1	response regulator aspartate phosphatase RapF	Regulator
I33_RS18100	phrF	3650348..3650467 (+)	120	WP_009968329.1	phosphatase RapF inhibitor PhrF	Regulator
I33_RS18105 (I33_3893)	ywhH	3650566..3651039 (+)	474	Protein_3645	YbaK/EbsC family protein	-
I33_RS18110 (I33_3894)	speB	3651070..3651942 (-)	873	WP_003227545.1	agmatinase	-
I33_RS18115 (I33_3895)	speE	3652003..3652833 (-)	831	WP_003227543.1	spermidine synthase	-
I33_RS18120 (I33_3896)	pbpG	3653036..3655111 (+)	2076	WP_014478298.1	transglycosylase domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45579.04 Da
• Isoelectric Point: 5.1464

>NTDB_id=45447 I33_RS18095 WP_014478296.1 3649219..3650364(+) (rapF) [Bacillus subtilis subsp. subtilis str. RO-NN-1]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGVLDYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKENEAYNIRLLQCHSLFATNFLDLKQYEDAISHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AITYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKMDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFNFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=45447 I33_RS18095 WP_014478296.1 3649219..3650364(+) (rapF) [Bacillus subtilis subsp. subtilis str. RO-NN-1]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTGATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGAGTCCTTGATTATTACTTTAATTTCTTCAG
GGGCATGTATGAGCTGGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAGAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAAAATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCATCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGAAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATAACTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATGGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTAATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

• Tetratricopeptide repeat (TPR_12) (223-290)

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3) (132-208)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	98.425	100	0.984
rapC	Bacillus subtilis subsp. subtilis str. 168	57.368	99.738	0.572