Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   I33_RS12430 Genome accession   NC_017195
Coordinates   2500013..2500423 (-) Length   136 a.a.
NCBI ID   WP_014477402.1    Uniprot ID   -
Organism   Bacillus subtilis subsp. subtilis str. RO-NN-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2495013..2505423
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I33_RS12400 (I33_2656) - 2495101..2495241 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  I33_RS12405 (I33_2657) yqeF 2495547..2496278 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  I33_RS12410 (I33_2659) cwlH 2496529..2497281 (-) 753 WP_014477398.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  I33_RS12415 (I33_2660) yqeD 2497468..2498094 (+) 627 WP_014477399.1 TVP38/TMEM64 family protein -
  I33_RS12420 (I33_2661) gnd 2498113..2499006 (-) 894 WP_014477400.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  I33_RS12425 (I33_2662) yqeB 2499258..2499980 (+) 723 WP_014477401.1 hypothetical protein -
  I33_RS12430 (I33_2663) nucA/comI 2500013..2500423 (-) 411 WP_014477402.1 sporulation-specific Dnase NucB Machinery gene
  I33_RS12435 (I33_2664) sigK 2500619..2501347 (+) 729 WP_013308023.1 RNA polymerase sporulation sigma factor SigK -
  I33_RS20995 - 2501347..2501463 (+) 117 WP_157863915.1 hypothetical protein -
  I33_RS12440 - 2501429..2501626 (-) 198 Protein_2490 recombinase family protein -
  I33_RS21345 - 2501584..2501762 (-) 179 Protein_2491 hypothetical protein -
  I33_RS21530 - 2502284..2502674 (+) 391 Protein_2492 SMI1/KNR4 family protein -
  I33_RS12450 (I33_2668) - 2502719..2503264 (-) 546 WP_014112744.1 TetR/AcrR family transcriptional regulator -
  I33_RS12455 (I33_2669) - 2503489..2504613 (+) 1125 WP_014477406.1 alpha/beta hydrolase -
  I33_RS12460 (I33_2670) - 2504783..2505058 (+) 276 WP_014477407.1 HPr family phosphocarrier protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14977.95 Da        Isoelectric Point: 5.1853

>NTDB_id=45426 I33_RS12430 WP_014477402.1 2500013..2500423(-) (nucA/comI) [Bacillus subtilis subsp. subtilis str. RO-NN-1]
MKKWLAGLFLAAAVLLCLMVPQQIQGASSYDRVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=45426 I33_RS12430 WP_014477402.1 2500013..2500423(-) (nucA/comI) [Bacillus subtilis subsp. subtilis str. RO-NN-1]
ATGAAAAAATGGTTGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
TTCTTCGTATGACAGAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCAGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGTTATCCTGACGGCACCAGAGTGCTATTTA
TTGTGCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529

Multiple sequence alignment