Detailed information NTG0454154

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: HUO02_RS12150
• Genome accession: NZ_CP054714
• Coordinates: 2510501..2511115 (-)
• Length: 204 a.a.
• NCBI ID: WP_012118026.1
• Uniprot ID: A7Z6X3
• Organism: Bacillus velezensis strain KKLW
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2505501..2516115

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HUO02_RS12120 (HUO02_12120)	rpsT	2505804..2506070 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
HUO02_RS12125 (HUO02_12125)	holA	2506087..2507128 (-)	1042	Protein_2357	DNA polymerase III subunit delta	-
HUO02_RS12130 (HUO02_12130)	-	2507168..2507319 (-)	152	Protein_2358	hypothetical protein	-
HUO02_RS12135 (HUO02_12135)	-	2507360..2507494 (+)	135	WP_003152870.1	YqzM family protein	-
HUO02_RS12140 (HUO02_12140)	comEC	2507513..2509864 (-)	2352	WP_012118025.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
HUO02_RS12145 (HUO02_12145)	-	2509865..2510434 (-)	570	WP_003152868.1	ComE operon protein 2	-
HUO02_RS12150 (HUO02_12150)	comEA	2510501..2511115 (-)	615	WP_012118026.1	helix-hairpin-helix domain-containing protein	Machinery gene
HUO02_RS12155 (HUO02_12155)	comER	2511174..2511995 (+)	822	WP_012118027.1	late competence protein ComER	-
HUO02_RS12160 (HUO02_12160)	-	2512064..2512801 (-)	738	WP_012118028.1	class I SAM-dependent methyltransferase	-
HUO02_RS12165 (HUO02_12165)	rsfS	2512798..2513154 (-)	357	WP_007408260.1	ribosome silencing factor	-
HUO02_RS12170 (HUO02_12170)	yqeK	2513171..2513731 (-)	561	WP_012118029.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
HUO02_RS12175 (HUO02_12175)	-	2513721..2514290 (-)	570	WP_012118030.1	nicotinate-nucleotide adenylyltransferase	-
HUO02_RS12180 (HUO02_12180)	yhbY	2514301..2514591 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
HUO02_RS12185 (HUO02_12185)	aroE	2514585..2515427 (-)	843	WP_012118031.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22195.40 Da
• Isoelectric Point: 6.4888

>NTDB_id=454154 HUO02_RS12150 WP_012118026.1 2510501..2511115(-) (comEA) [Bacillus velezensis strain KKLW]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINMASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=454154 HUO02_RS12150 WP_012118026.1 2510501..2511115(-) (comEA) [Bacillus velezensis strain KKLW]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATATGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

3D structure

• AlphaFold DB: A7Z6X3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363