Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HUG20_RS00665 Genome accession   NZ_CP054706
Coordinates   136946..138331 (+) Length   461 a.a.
NCBI ID   WP_200086860.1    Uniprot ID   -
Organism   Salicibibacter cibi strain NKC21-4     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 131946..143331
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUG20_RS00645 (HUG20_00645) - 132281..132751 (+) 471 WP_200086854.1 CtsR family transcriptional regulator -
  HUG20_RS00650 (HUG20_00650) - 132772..133314 (+) 543 WP_200086856.1 UvrB/UvrC motif-containing protein -
  HUG20_RS00655 (HUG20_00655) - 133314..134402 (+) 1089 WP_200086858.1 protein arginine kinase -
  HUG20_RS00660 (HUG20_00660) - 134399..136855 (+) 2457 WP_211199997.1 ATP-dependent Clp protease ATP-binding subunit -
  HUG20_RS00665 (HUG20_00665) radA 136946..138331 (+) 1386 WP_200086860.1 DNA repair protein RadA Machinery gene
  HUG20_RS00670 (HUG20_00670) disA 138350..139423 (+) 1074 WP_200086863.1 DNA integrity scanning diadenylate cyclase DisA -
  HUG20_RS00675 (HUG20_00675) - 139575..140693 (+) 1119 WP_200090329.1 PIN/TRAM domain-containing protein -
  HUG20_RS00680 (HUG20_00680) ispD 141084..141800 (+) 717 WP_200086865.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  HUG20_RS00685 (HUG20_00685) ispF 141778..142251 (+) 474 WP_200086867.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50364.73 Da        Isoelectric Point: 6.1066

>NTDB_id=454082 HUG20_RS00665 WP_200086860.1 136946..138331(+) (radA) [Salicibibacter cibi strain NKC21-4]
MAKTKTKFVCQECTYESRKWMGRCPSCQNWNTLVEEMELDEPKRTSGTVRSVGSNRPQKISQINSQDEPRMTTKLSELNR
VLGGGIVPGSLVLVGGDPGIGKSTLLLQLSALLAEDGRKVLYISGEESVKQTKMRADRLKAGADALYVLTETDTSLIDRA
VDMVEPDVLIIDSVQTMQTPDIASAPGSVAQVRESTSAFMSMAKSRGISVFVVGHVTKQGSIAGPKMLEHMVDAVLYFEG
ERHHTFRILRAVKNRFGSTNEIGIFEMNEEGLREVLNPSEIFLEERSEGVAGATVVASLEGTRPVLVELQALVAPTSYAN
PRRTATGVDHNRVSLLMAVLERRVGMLLQNQDAYINVAGGVKLDEPAVDLALATSIASSFRNQVTRPTDVAIGEVGLTGE
IRRVSRIQERVKESAKLGFDRCIIPEKNMGGWKAPDQMTCIGVGSLEEALDVMLEQRAKTW

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=454082 HUG20_RS00665 WP_200086860.1 136946..138331(+) (radA) [Salicibibacter cibi strain NKC21-4]
ATGGCGAAAACAAAAACCAAGTTTGTTTGCCAGGAATGTACGTATGAATCCCGGAAGTGGATGGGCCGTTGCCCTTCATG
CCAGAATTGGAACACGCTAGTAGAGGAGATGGAATTGGATGAGCCAAAACGTACCAGTGGTACGGTGCGTTCCGTCGGCA
GTAATCGCCCGCAAAAAATCAGTCAGATAAACAGCCAAGACGAACCGCGAATGACAACGAAATTATCAGAATTAAATCGT
GTTTTAGGCGGGGGAATTGTTCCCGGCTCCCTGGTTCTTGTCGGAGGCGACCCCGGCATTGGCAAGTCAACGCTGCTTTT
GCAACTTTCCGCACTGTTGGCAGAAGACGGAAGGAAAGTGTTATATATATCCGGAGAGGAATCGGTAAAACAAACAAAAA
TGCGTGCGGATCGTTTGAAGGCAGGAGCGGATGCCTTGTACGTGCTTACCGAAACCGATACTTCATTGATTGATCGAGCA
GTGGATATGGTTGAACCGGACGTGTTGATCATAGATTCCGTTCAAACGATGCAAACGCCGGACATTGCCTCTGCCCCCGG
AAGCGTTGCACAAGTGCGCGAGTCGACATCTGCTTTTATGAGCATGGCAAAATCCCGGGGCATTTCGGTGTTTGTTGTTG
GACATGTGACGAAACAAGGGTCGATTGCAGGACCGAAAATGCTGGAACATATGGTGGATGCGGTTCTATATTTTGAAGGG
GAGCGGCACCATACGTTTCGAATTTTGCGGGCAGTGAAAAATCGCTTTGGTTCTACAAATGAAATTGGCATATTTGAAAT
GAATGAAGAAGGTTTGCGGGAAGTGTTAAATCCGTCGGAAATTTTCTTGGAAGAGCGTTCGGAAGGGGTGGCGGGTGCAA
CTGTTGTGGCCTCCCTTGAAGGAACCCGCCCCGTTTTGGTGGAGCTGCAAGCCCTCGTTGCCCCGACTAGCTATGCCAAT
CCCCGGCGTACGGCAACCGGTGTTGACCATAACCGTGTTTCCTTGCTGATGGCGGTATTGGAAAGGCGCGTGGGAATGTT
GCTTCAAAATCAAGATGCTTATATTAATGTTGCAGGCGGCGTAAAATTGGATGAACCGGCCGTAGATTTGGCATTGGCGA
CGAGTATCGCTTCGAGTTTTCGCAATCAAGTGACACGTCCGACTGATGTTGCGATCGGTGAAGTAGGCTTAACAGGGGAA
ATCCGCAGGGTCTCCCGTATTCAGGAAAGGGTAAAAGAAAGCGCGAAACTAGGGTTTGACCGATGCATCATTCCGGAAAA
AAATATGGGTGGTTGGAAAGCTCCCGATCAGATGACATGTATAGGCGTTGGTTCCTTGGAAGAGGCATTGGATGTGATGC
TTGAACAACGGGCGAAAACTTGGTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(68-220)

Subunit ChlI of Mg-chelatase (ChlI)

(344-428)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.009

98.915

0.653
  radA Streptococcus mitis NCTC 12261

56.984

97.831

0.557
  radA Streptococcus pneumoniae Rx1

56.984

97.831

0.557
  radA Streptococcus pneumoniae D39

56.984

97.831

0.557
  radA Streptococcus pneumoniae R6

56.984

97.831

0.557
  radA Streptococcus pneumoniae TIGR4

56.984

97.831

0.557
  radA Streptococcus mitis SK321

56.984

97.831

0.557