Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HUE58_RS00230 Genome accession   NZ_CP054490
Coordinates   47919..48875 (-) Length   318 a.a.
NCBI ID   WP_174605105.1    Uniprot ID   A0A6N0HMU5
Organism   Candidatus Ruthia endofausta isolate JDF_Ridge     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 42919..53875
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUE58_RS00205 (HUE58_00205) argH 43337..44779 (-) 1443 WP_174605100.1 argininosuccinate lyase -
  HUE58_RS00210 (HUE58_00210) hemC 45133..46047 (+) 915 WP_174605101.1 hydroxymethylbilane synthase -
  HUE58_RS00215 (HUE58_00215) - 46051..46335 (+) 285 WP_174605102.1 4a-hydroxytetrahydrobiopterin dehydratase -
  HUE58_RS00220 (HUE58_00220) - 46337..47014 (+) 678 WP_174605103.1 hypothetical protein -
  HUE58_RS00225 (HUE58_00225) - 47510..47776 (-) 267 WP_174605104.1 hypothetical protein -
  HUE58_RS00230 (HUE58_00230) pilA 47919..48875 (-) 957 WP_174605105.1 signal recognition particle-docking protein FtsY Machinery gene
  HUE58_RS00235 (HUE58_00235) - 49288..49530 (+) 243 WP_174605064.1 hypothetical protein -
  HUE58_RS00240 (HUE58_00240) - 49527..50852 (+) 1326 WP_174605106.1 pitrilysin family protein -
  HUE58_RS00245 (HUE58_00245) - 50849..52138 (+) 1290 WP_174605107.1 pitrilysin family protein -
  HUE58_RS06715 - 52200..52376 (+) 177 WP_246260809.1 hypothetical protein -
  HUE58_RS00255 (HUE58_00255) - 52567..52647 (+) 81 Protein_48 hypothetical protein -

Sequence

Protein


Download         Length: 318 a.a.        Molecular weight: 34679.57 Da        Isoelectric Point: 8.3570

>NTDB_id=452851 HUE58_RS00230 WP_174605105.1 47919..48875(-) (pilA) [Candidatus Ruthia endofausta isolate JDF_Ridge]
MFNFFKKNQSKSEGLDADKPASLRQRLNKSRQKLGSGLSSLLLGKKEINDDLLEALETLLITADVGINTTDKILESVRKN
ASRNTLKDAENLYQFLKDELAKLLLEDNQLNTNINETFVILVVGVNGAGKTTLIGKMAKSFQNQGKSVMLAAGDTFRAAA
VEQLRVWGQRNNTPVVAQTTGTDAASVIFDAYQSAQAKNIDILIADTAGRLHTQNNLMQELAKIKRVIAKQNENAPHETM
LVIDGGSGQNVINQAKEFNKAVTLSGISITKLDGTAKGGILFAIADELNLPIRYIGVGEEINDLKAFQSKEFINALFD

Nucleotide


Download         Length: 957 bp        

>NTDB_id=452851 HUE58_RS00230 WP_174605105.1 47919..48875(-) (pilA) [Candidatus Ruthia endofausta isolate JDF_Ridge]
ATGTTTAATTTTTTTAAAAAAAATCAGTCTAAATCAGAAGGTTTAGACGCAGACAAGCCAGCTTCATTAAGACAACGTCT
AAATAAATCTAGACAAAAACTAGGGTCTGGCTTATCTTCTTTATTATTAGGTAAAAAAGAAATTAATGACGATTTATTAG
AGGCATTAGAAACCCTATTAATCACTGCTGATGTTGGCATTAATACCACAGATAAAATACTAGAGTCTGTACGTAAAAAT
GCCTCTAGAAACACCTTAAAAGATGCTGAAAACTTGTATCAATTCTTAAAAGATGAATTGGCTAAATTGCTCCTTGAAGA
TAACCAGCTTAATACTAATATTAATGAGACTTTTGTTATTCTAGTCGTAGGTGTTAATGGCGCTGGCAAAACCACCTTGA
TTGGCAAAATGGCAAAATCTTTTCAAAATCAAGGCAAGTCGGTTATGTTGGCAGCAGGCGATACCTTTCGTGCCGCTGCT
GTTGAACAACTCAGAGTGTGGGGGCAGCGCAATAACACACCTGTTGTAGCACAAACAACGGGTACCGATGCAGCTTCAGT
TATCTTTGATGCCTACCAATCAGCACAAGCTAAAAATATTGATATTTTAATTGCCGACACTGCAGGGCGATTACATACAC
AGAACAACCTTATGCAAGAACTAGCCAAAATTAAGCGTGTCATTGCGAAACAAAATGAAAACGCACCGCATGAAACCATG
CTGGTTATTGACGGCGGATCAGGGCAAAATGTCATCAATCAAGCTAAAGAATTTAACAAAGCAGTTACTTTAAGCGGCAT
TAGTATCACTAAGCTAGATGGCACCGCCAAAGGCGGGATTTTATTTGCAATTGCCGATGAACTTAATCTGCCCATTCGTT
ATATTGGTGTAGGTGAAGAGATTAACGATCTTAAAGCTTTTCAATCCAAAGAATTCATCAACGCTTTGTTTGATTAA

Domains

Predicted by InterproScan.

SRP54-type protein, GTPase domain (SRP54)

(118-317)

SRP54-type protein, helical bundle domain (SRP54_N)

(34-100)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N0HMU5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

53

94.34

0.5