Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   HT132_RS18870 Genome accession   NZ_CP054479
Coordinates   3849222..3849659 (+) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain R8-25     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3844222..3854659
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HT132_RS18855 (HT132_18855) - 3847108..3847470 (-) 363 WP_007409352.1 helix-turn-helix domain-containing protein -
  HT132_RS18860 (HT132_18860) hxlA 3847702..3848337 (+) 636 WP_174532720.1 3-hexulose-6-phosphate synthase -
  HT132_RS18865 (HT132_18865) hxlB 3848334..3848891 (+) 558 WP_014416874.1 6-phospho-3-hexuloisomerase -
  HT132_RS18870 (HT132_18870) nucA/comI 3849222..3849659 (+) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  HT132_RS18875 (HT132_18875) nin/comJ 3849680..3850078 (+) 399 WP_174532721.1 competence protein ComJ Regulator
  HT132_RS18880 (HT132_18880) - 3850118..3851554 (-) 1437 WP_174532722.1 glycoside hydrolase family 1 protein -
  HT132_RS18885 (HT132_18885) - 3851658..3851981 (-) 324 WP_007409357.1 YckD family protein -
  HT132_RS18890 (HT132_18890) - 3852053..3852487 (-) 435 WP_021494203.1 RDD family protein -
  HT132_RS18895 (HT132_18895) - 3852502..3853638 (-) 1137 WP_174532723.1 zinc-dependent alcohol dehydrogenase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=452812 HT132_RS18870 WP_003156588.1 3849222..3849659(+) (nucA/comI) [Bacillus amyloliquefaciens strain R8-25]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=452812 HT132_RS18870 WP_003156588.1 3849222..3849659(+) (nucA/comI) [Bacillus amyloliquefaciens strain R8-25]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAGGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641