Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   HT132_RS11925 Genome accession   NZ_CP054479
Coordinates   2378787..2379221 (-) Length   144 a.a.
NCBI ID   WP_021493650.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain R8-25     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2373787..2384221
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HT132_RS11890 (HT132_11890) - 2373802..2374155 (+) 354 WP_029973435.1 multidrug efflux SMR transporter -
  HT132_RS11895 (HT132_11895) - 2374169..2374621 (-) 453 WP_014305039.1 OsmC family protein -
  HT132_RS11900 (HT132_11900) - 2374681..2375388 (-) 708 WP_014417828.1 poly-gamma-glutamate hydrolase family protein -
  HT132_RS11905 (HT132_11905) - 2375644..2375877 (-) 234 WP_038461471.1 hypothetical protein -
  HT132_RS11910 (HT132_11910) - 2376056..2377384 (+) 1329 WP_160216862.1 S8 family peptidase -
  HT132_RS11915 (HT132_11915) - 2377576..2377938 (+) 363 WP_007410383.1 hypothetical protein -
  HT132_RS11920 (HT132_11920) - 2377972..2378727 (-) 756 WP_022553315.1 YoaK family protein -
  HT132_RS11925 (HT132_11925) nucA/comI 2378787..2379221 (-) 435 WP_021493650.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  HT132_RS11930 (HT132_11930) - 2379510..2380721 (+) 1212 WP_174532547.1 cytochrome P450 -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15480.42 Da        Isoelectric Point: 7.2418

>NTDB_id=452784 HT132_RS11925 WP_021493650.1 2378787..2379221(-) (nucA/comI) [Bacillus amyloliquefaciens strain R8-25]
MNAFMKWAASLLLVISLQVGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=452784 HT132_RS11925 WP_021493650.1 2378787..2379221(-) (nucA/comI) [Bacillus amyloliquefaciens strain R8-25]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGGTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATACT
CCGACGGCACGAGAGTACTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486