Detailed information NTG0452769

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: HT132_RS08220
• Genome accession: NZ_CP054479
• Coordinates: 1600798..1601412 (+)
• Length: 204 a.a.
• NCBI ID: WP_172870187.1
• Uniprot ID: -
• Organism: Bacillus amyloliquefaciens strain R8-25
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1595798..1606412

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HT132_RS08185 (HT132_08185)	aroE	1596485..1597327 (+)	843	WP_003152856.1	shikimate dehydrogenase	-
HT132_RS08190 (HT132_08190)	yhbY	1597321..1597611 (+)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
HT132_RS08195 (HT132_08195)	-	1597622..1598191 (+)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
HT132_RS08200 (HT132_08200)	yqeK	1598181..1598741 (+)	561	WP_174532484.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
HT132_RS08205 (HT132_08205)	rsfS	1598759..1599115 (+)	357	WP_007408260.1	ribosome silencing factor	-
HT132_RS08210 (HT132_08210)	-	1599112..1599849 (+)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
HT132_RS08215 (HT132_08215)	comER	1599918..1600739 (-)	822	WP_012118027.1	late competence protein ComER	-
HT132_RS08220 (HT132_08220)	comEA	1600798..1601412 (+)	615	WP_172870187.1	helix-hairpin-helix domain-containing protein	Machinery gene
HT132_RS08225 (HT132_08225)	-	1601479..1602048 (+)	570	WP_003152868.1	ComE operon protein 2	-
HT132_RS08230 (HT132_08230)	comEC	1602049..1604400 (+)	2352	WP_174532485.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
HT132_RS08235 (HT132_08235)	-	1604419..1604553 (-)	135	WP_003152870.1	YqzM family protein	-
HT132_RS08240 (HT132_08240)	-	1604594..1604745 (+)	152	Protein_1582	hypothetical protein	-
HT132_RS08245 (HT132_08245)	holA	1604785..1605880 (+)	1096	Protein_1583	DNA polymerase III subunit delta	-
HT132_RS08250 (HT132_08250)	rpsT	1605843..1606109 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22225.32 Da
• Isoelectric Point: 5.5211

>NTDB_id=452769 HT132_RS08220 WP_172870187.1 1600798..1601412(+) (comEA) [Bacillus amyloliquefaciens strain R8-25]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=452769 HT132_RS08220 WP_172870187.1 1600798..1601412(+) (comEA) [Bacillus amyloliquefaciens strain R8-25]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363