Detailed information    

insolico Bioinformatically predicted

Overview

Name   rapF   Type   Regulator
Locus tag   HT132_RS02810 Genome accession   NZ_CP054479
Coordinates   520484..521629 (-) Length   381 a.a.
NCBI ID   WP_099721980.1    Uniprot ID   A0AAP3YDZ8
Organism   Bacillus amyloliquefaciens strain R8-25     
Function   inhibit the DNA-binding function of ComA (predicted from homology)   
Competence regulation

Genomic Context

Location: 515484..526629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HT132_RS02765 (HT132_02765) speE 515794..516624 (+) 831 WP_003151052.1 spermidine synthase -
  HT132_RS02770 (HT132_02770) speB 516683..517555 (+) 873 WP_007614448.1 agmatinase -
  HT132_RS02775 (HT132_02775) - 517710..517928 (+) 219 WP_003151056.1 LuxR C-terminal-related transcriptional regulator -
  HT132_RS02780 (HT132_02780) - 518519..519031 (-) 513 WP_007614439.1 stage II sporulation protein M -
  HT132_RS02785 (HT132_02785) - 519075..519356 (-) 282 WP_007407643.1 hypothetical protein -
  HT132_RS02790 (HT132_02790) - 519543..519728 (-) 186 WP_007407642.1 DUF4177 domain-containing protein -
  HT132_RS02795 (HT132_02795) - 519763..519966 (-) 204 WP_007407641.1 hypothetical protein -
  HT132_RS02800 (HT132_02800) - 519980..520183 (-) 204 WP_003151064.1 helix-turn-helix transcriptional regulator -
  HT132_RS02805 (HT132_02805) - 520381..520500 (-) 120 WP_021494790.1 PhrC/PhrF family phosphatase-inhibitory pheromone -
  HT132_RS02810 (HT132_02810) rapF 520484..521629 (-) 1146 WP_099721980.1 tetratricopeptide repeat protein Regulator
  HT132_RS02815 (HT132_02815) - 521921..522349 (+) 429 WP_100262065.1 DUF1934 domain-containing protein -
  HT132_RS02820 (HT132_02820) argS 522346..524016 (+) 1671 WP_014419127.1 arginine--tRNA ligase -
  HT132_RS02825 (HT132_02825) narK 524150..525340 (+) 1191 WP_007407632.1 nitrate transporter NarK -
  HT132_RS02830 (HT132_02830) - 525438..526154 (+) 717 WP_007407631.1 Crp/Fnr family transcriptional regulator -

Sequence

Protein


Download         Length: 381 a.a.        Molecular weight: 45567.62 Da        Isoelectric Point: 4.5769

>NTDB_id=452748 HT132_RS02810 WP_099721980.1 520484..521629(-) (rapF) [Bacillus amyloliquefaciens strain R8-25]
MASVLSSSAVGEKINEWYMYIRRFSIPDAEYLRREIKEELAGLEDDQDLHLYYSLMEFRHNLMLEYLEPLESQRIEEQPR
LSDLLADIDKKQARLTGRLDYYFNFFRGMYELECREYLSAIQFFKKAEYKLDFVKDCIEKAEFYFKMSESYYYMKQTYFS
MDYARQAYKIYHKQEAYNIRVLQCHSLFATNFLDLKQYDEAIQHFKKAYAMAEAEQQPQLMGRTLYNIGLCFNSQENYKP
AIDYIKRAIAVFEDGNIITSLPQAYFLITQIHYKIGNTAIARQYHDKGVSYAEEAEDSLYIVEYEFLESLYVGEPDEEAI
MECFDFLRDKLMYADLEDFALDVAKYYHERENFEKASAYFLKVEETRQQIQGGVKLYEIEV

Nucleotide


Download         Length: 1146 bp        

>NTDB_id=452748 HT132_RS02810 WP_099721980.1 520484..521629(-) (rapF) [Bacillus amyloliquefaciens strain R8-25]
GTGGCGAGTGTTTTATCGTCTTCAGCGGTTGGAGAAAAAATCAACGAATGGTATATGTACATTCGAAGGTTCAGCATCCC
GGACGCCGAGTACTTACGGAGAGAAATCAAAGAAGAACTTGCAGGCCTGGAAGATGATCAGGATCTTCATCTATACTATT
CTCTCATGGAATTCCGCCACAATCTGATGCTCGAATACCTCGAACCGCTGGAATCGCAGCGGATAGAAGAACAGCCGAGA
CTATCGGATCTGCTCGCGGATATTGATAAAAAGCAGGCCCGGCTGACCGGACGTCTTGATTATTATTTTAATTTTTTCCG
GGGGATGTATGAGCTGGAATGCCGTGAATATCTTTCAGCGATTCAGTTTTTTAAGAAAGCTGAATATAAGCTTGATTTTG
TCAAAGATTGTATCGAGAAAGCTGAATTTTATTTTAAGATGTCAGAGTCCTACTATTATATGAAACAGACTTATTTTTCT
ATGGATTATGCCCGGCAGGCTTATAAAATTTATCATAAGCAGGAGGCATATAATATAAGGGTGCTTCAGTGCCATTCATT
GTTCGCTACCAACTTTCTTGACTTAAAGCAATATGATGAAGCAATTCAGCATTTTAAAAAAGCTTATGCCATGGCAGAGG
CAGAACAGCAGCCTCAATTAATGGGCCGGACTCTATATAATATCGGACTTTGTTTTAACAGCCAAGAAAATTACAAACCA
GCCATTGACTACATAAAACGGGCAATAGCTGTTTTTGAAGATGGGAACATCATCACTTCTCTCCCGCAAGCCTATTTCTT
AATCACACAAATACATTATAAAATCGGAAATACGGCCATAGCCAGACAATATCATGACAAAGGGGTATCTTATGCAGAGG
AGGCAGAAGATTCCCTATATATAGTCGAGTATGAATTTTTAGAATCCTTATATGTCGGTGAGCCTGATGAAGAAGCAATC
ATGGAATGTTTTGACTTTCTCAGAGATAAATTGATGTATGCTGATCTTGAAGATTTTGCCTTAGATGTGGCAAAATATTA
TCATGAAAGAGAAAATTTCGAGAAGGCTTCCGCATATTTTTTAAAGGTGGAAGAAACAAGACAGCAGATTCAAGGAGGTG
TGAAGTTGTATGAAATTGAAGTATAA

Domains

Predicted by InterproScan.

response regulator aspartate phosphatase H, N terminal (RapH_N)

(5-65)

Tetratricopeptide repeat (TPR_12)

(189-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rapF Bacillus subtilis subsp. subtilis str. 168

78.478

100

0.785
  rapC Bacillus subtilis subsp. subtilis str. 168

56.842

99.738

0.567