Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HT051_RS08330 Genome accession   NZ_CP054476
Coordinates   1759194..1760573 (-) Length   459 a.a.
NCBI ID   WP_174541605.1    Uniprot ID   A0A6N0DTS3
Organism   Methyloligella sp. GL2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1754194..1765573
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HT051_RS08305 (HT051_08305) - 1754225..1755373 (-) 1149 WP_174541600.1 autotransporter outer membrane beta-barrel domain-containing protein -
  HT051_RS08310 (HT051_08310) - 1755565..1755978 (+) 414 WP_174541601.1 DUF4440 domain-containing protein -
  HT051_RS08315 (HT051_08315) - 1756000..1756704 (-) 705 WP_174541602.1 SDR family NAD(P)-dependent oxidoreductase -
  HT051_RS08320 (HT051_08320) purF 1756754..1758241 (-) 1488 WP_246810027.1 amidophosphoribosyltransferase -
  HT051_RS08325 (HT051_08325) - 1758434..1759033 (-) 600 WP_174541604.1 CvpA family protein -
  HT051_RS08330 (HT051_08330) radA 1759194..1760573 (-) 1380 WP_174541605.1 DNA repair protein RadA Machinery gene
  HT051_RS08335 (HT051_08335) alr 1760577..1761740 (-) 1164 WP_246810028.1 alanine racemase -
  HT051_RS08340 (HT051_08340) - 1761737..1763224 (-) 1488 WP_174541606.1 replicative DNA helicase -
  HT051_RS08345 (HT051_08345) - 1763392..1764651 (-) 1260 WP_174541607.1 cyclopropane-fatty-acyl-phospholipid synthase family protein -
  HT051_RS08350 (HT051_08350) rplI 1764830..1765474 (-) 645 WP_174541608.1 50S ribosomal protein L9 -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 47714.53 Da        Isoelectric Point: 5.5226

>NTDB_id=452731 HT051_RS08330 WP_174541605.1 1759194..1760573(-) (radA) [Methyloligella sp. GL2]
MAKATRNFICQECGAVSTRWAGKCVTCGAWNSMVEETPAPPATANLVSIKGGKGRAAKFESLEAETLDAPRIPSGIGEFD
RVTGGGLVPGSAILVGGEPGIGKSTLLLQTAAKLATAGQPVVYLSGEEAPAQVRMRAERLGLSKAPVALGTETNTANIIA
SLSEGEAPALIIIDSVQTLWAENIDSAPGTISQLRGCAAALISFAKRTGSTLILVGHVTKEGQIAGPKVIEHMVDTVLYF
EGQSGHQFRILRGVKNRFGPTDEIGVFEMRYDGLNEVENPSALFLSDRDEASPGTSVLAGMEGSRPILVEIQALVAPSSL
GTPRRAVVGWDTSRLAMLIAVLEARCGVRLGGHDVYLNVAGGLRLNEPAVDLAAAAALVSSFANKSVPEGSVYFGEVSLT
GAIRPVGHGETRLKEAAKLGFSRAVLPASAVPQAKSAGLAHAEVSELVELVSQFGPFDE

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=452731 HT051_RS08330 WP_174541605.1 1759194..1760573(-) (radA) [Methyloligella sp. GL2]
TTGGCCAAAGCGACACGCAATTTCATCTGCCAGGAATGCGGCGCCGTCAGCACCCGCTGGGCCGGCAAATGCGTCACCTG
CGGCGCCTGGAACTCCATGGTCGAGGAGACCCCGGCCCCGCCGGCCACGGCCAATCTCGTTTCCATCAAAGGCGGCAAGG
GCCGCGCGGCGAAATTCGAGAGCCTGGAAGCGGAGACCCTGGATGCCCCGCGCATCCCCTCCGGCATCGGCGAGTTCGAC
CGGGTGACCGGCGGCGGGCTGGTGCCGGGCTCGGCCATCCTGGTCGGCGGCGAGCCGGGCATCGGCAAGTCGACCTTGCT
GCTGCAGACCGCAGCGAAACTCGCCACTGCCGGCCAGCCGGTGGTCTATCTCTCCGGCGAAGAGGCCCCGGCCCAGGTGC
GCATGCGCGCCGAACGGCTCGGCCTCTCCAAGGCGCCCGTAGCACTCGGCACCGAGACCAACACCGCCAATATCATCGCC
AGCCTCTCCGAGGGCGAAGCGCCGGCGCTGATCATCATCGATTCGGTACAGACCCTGTGGGCGGAGAATATCGATTCGGC
GCCCGGCACCATCTCCCAGCTGCGCGGCTGCGCCGCGGCGCTGATCTCCTTTGCCAAGCGCACCGGCTCGACCCTGATTC
TGGTCGGCCACGTCACCAAGGAAGGCCAGATCGCCGGCCCCAAGGTGATCGAGCATATGGTCGATACCGTGCTCTATTTC
GAAGGCCAGAGCGGCCACCAATTCCGCATCCTCCGCGGCGTCAAGAATCGCTTCGGCCCCACCGACGAGATCGGCGTCTT
CGAGATGCGCTATGACGGGCTGAACGAGGTGGAGAACCCCTCGGCCTTGTTTCTAAGCGACCGCGACGAAGCCAGCCCCG
GCACCTCAGTGCTGGCCGGCATGGAAGGCAGCCGCCCCATCCTGGTGGAGATCCAGGCCCTGGTCGCGCCCTCCTCTCTC
GGCACGCCGCGCCGCGCCGTGGTCGGCTGGGACACCAGCCGCCTGGCCATGCTGATCGCCGTGCTGGAAGCGCGCTGCGG
CGTGCGCCTCGGCGGCCATGACGTCTATCTCAACGTCGCCGGCGGCTTGCGGCTCAACGAGCCCGCCGTGGATCTGGCCG
CCGCCGCCGCGCTTGTTTCTTCCTTCGCCAATAAATCCGTGCCCGAGGGCAGCGTCTATTTCGGTGAGGTCAGTCTCACC
GGTGCGATTCGCCCCGTGGGCCATGGCGAAACCCGGCTGAAGGAAGCGGCCAAGCTCGGCTTCTCCCGCGCCGTGCTGCC
GGCCAGCGCCGTGCCGCAAGCCAAATCCGCCGGCCTCGCCCATGCCGAAGTGAGCGAGCTGGTGGAGCTGGTCAGCCAGT
TCGGTCCTTTCGACGAATAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(346-429)

AAA domain (AAA_25)

(82-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6N0DTS3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.342

95.643

0.481
  radA Streptococcus pneumoniae Rx1

46.868

93.9

0.44
  radA Streptococcus pneumoniae D39

46.868

93.9

0.44
  radA Streptococcus pneumoniae R6

46.868

93.9

0.44
  radA Streptococcus pneumoniae TIGR4

46.868

93.9

0.44
  radA Streptococcus mitis SK321

46.868

93.9

0.44
  radA Streptococcus mitis NCTC 12261

46.636

93.9

0.438