Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   HR084_RS12930 Genome accession   NZ_CP054177
Coordinates   2543608..2544018 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   A0A6I4D881
Organism   Bacillus subtilis strain JCL16     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2538608..2549018
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HR084_RS12900 (HR084_12900) - 2538695..2538835 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  HR084_RS12905 (HR084_12905) yqeF 2539141..2539872 (-) 732 WP_029317882.1 SGNH/GDSL hydrolase family protein -
  HR084_RS12910 (HR084_12910) cwlH 2540124..2540876 (-) 753 WP_160221685.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  HR084_RS12915 (HR084_12915) yqeD 2541063..2541689 (+) 627 WP_173614194.1 TVP38/TMEM64 family protein -
  HR084_RS12920 (HR084_12920) gnd 2541708..2542601 (-) 894 WP_043857850.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  HR084_RS12925 (HR084_12925) yqeB 2542853..2543575 (+) 723 WP_010886572.1 hypothetical protein -
  HR084_RS12930 (HR084_12930) nucA/comI 2543608..2544018 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  HR084_RS12935 (HR084_12935) - 2544214..2544561 (+) 348 Protein_2514 sigma-70 family RNA polymerase sigma factor -
  HR084_RS12940 (HR084_12940) spoIVCA 2544592..2546052 (-) 1461 WP_240304280.1 site-specific DNA recombinase SpoIVCA -
  HR084_RS21050 - 2546010..2546188 (-) 179 Protein_2516 hypothetical protein -
  HR084_RS12945 (HR084_12945) - 2546641..2547654 (-) 1014 WP_173614196.1 NADP-dependent oxidoreductase -
  HR084_RS12950 (HR084_12950) - 2548084..2548236 (-) 153 WP_260438897.1 hypothetical protein -
  HR084_RS12955 (HR084_12955) - 2548252..2549001 (-) 750 WP_173614197.1 SDR family NAD(P)-dependent oxidoreductase -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=451342 HR084_RS12930 WP_009967785.1 2543608..2544018(-) (nucA/comI) [Bacillus subtilis strain JCL16]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=451342 HR084_RS12930 WP_009967785.1 2543608..2544018(-) (nucA/comI) [Bacillus subtilis strain JCL16]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCAGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I4D881

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529