Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HQN60_RS01625 Genome accession   NZ_CP054143
Coordinates   321937..323319 (-) Length   460 a.a.
NCBI ID   WP_173532049.1    Uniprot ID   A0A6M8SRP3
Organism   Deefgea piscis strain D17     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 316937..328319
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HQN60_RS01590 (HQN60_01590) - 317565..318167 (-) 603 WP_173532044.1 flavin prenyltransferase UbiX -
  HQN60_RS01595 (HQN60_01595) - 318164..318703 (-) 540 WP_173532045.1 Gx transporter family protein -
  HQN60_RS01600 (HQN60_01600) - 318684..319091 (-) 408 WP_254456662.1 NusG domain II-containing protein -
  HQN60_RS01605 (HQN60_01605) rapZ 319069..319932 (-) 864 WP_173532046.1 RNase adapter RapZ -
  HQN60_RS01610 (HQN60_01610) hprK 319932..320876 (-) 945 WP_173532047.1 HPr(Ser) kinase/phosphatase -
  HQN60_RS01615 (HQN60_01615) ptsN 320989..321462 (-) 474 WP_173534556.1 PTS IIA-like nitrogen regulatory protein PtsN -
  HQN60_RS01620 (HQN60_01620) hpf 321567..321893 (-) 327 WP_173532048.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  HQN60_RS01625 (HQN60_01625) rpoN/rpoN1 321937..323319 (-) 1383 WP_173532049.1 RNA polymerase factor sigma-54 Machinery gene
  HQN60_RS01630 (HQN60_01630) lptB 323348..324070 (-) 723 WP_173532050.1 LPS export ABC transporter ATP-binding protein -
  HQN60_RS01635 (HQN60_01635) lptA 324067..324561 (-) 495 WP_173532051.1 lipopolysaccharide transport periplasmic protein LptA -
  HQN60_RS01640 (HQN60_01640) lptC 324518..325114 (-) 597 WP_173532052.1 LPS export ABC transporter periplasmic protein LptC -
  HQN60_RS01645 (HQN60_01645) - 325124..325639 (-) 516 WP_173532053.1 HAD family hydrolase -
  HQN60_RS01650 (HQN60_01650) - 325732..326718 (-) 987 WP_173532054.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 460 a.a.        Molecular weight: 52044.50 Da        Isoelectric Point: 4.6804

>NTDB_id=451102 HQN60_RS01625 WP_173532049.1 321937..323319(-) (rpoN/rpoN1) [Deefgea piscis strain D17]
MKQTLQLRMSQQLNLTPQLQQSIKLLQLSTLDFQQEIEQFLAENPMLEREDGVQDAGEEAEKPEVSEIVEDGDSLRWDEV
RSNASFDEGDENDPALRVAKLYSLRDYLLAQTGCLPLSARDNALISLLIDALDDDGMLSQSLEEIYAQLPESLVLELELE
PEELQIALQHLQQLEPLGVGARDLPESLTLQLRVLPESTIRNIAIRLVNEGLDLLAQRDYTRLKRQLQCDEVALKEAQIL
IRRLTPRPGANWSAETIRYVLPDVIVTKVRGQWQVRLNSAAQPKLRVNELYAKLLQGDSAAALSGKLQEARWLIKSVEQR
GSTILRVAEAIVMRQKAFFEYGEVAMRPLVLRDIADELELHESTISRVTTQKYMLTPRGVFEYKYFFGSHVESESGGENS
ATSIKALIKQLVQQENQHKPLSDSAITEELARQGVVVARRTVAKYREALMIPPVNLRKCL

Nucleotide


Download         Length: 1383 bp        

>NTDB_id=451102 HQN60_RS01625 WP_173532049.1 321937..323319(-) (rpoN/rpoN1) [Deefgea piscis strain D17]
ATGAAGCAAACCTTGCAGTTGCGGATGTCGCAACAGCTCAATTTGACACCGCAGCTTCAGCAATCGATTAAGCTCTTGCA
GTTGTCTACGCTTGATTTCCAACAAGAAATAGAGCAATTTTTAGCCGAAAATCCAATGCTTGAACGCGAAGATGGCGTGC
AAGATGCGGGTGAAGAAGCTGAAAAACCAGAGGTCAGTGAGATCGTTGAGGATGGCGATAGCTTGCGCTGGGATGAGGTG
CGCAGCAACGCCTCGTTTGATGAAGGGGATGAAAATGACCCCGCTTTGCGGGTGGCGAAGCTGTATTCGTTGCGTGATTA
TTTGTTAGCGCAAACTGGCTGCTTACCCTTGTCTGCTCGTGATAATGCATTGATTTCATTACTGATTGATGCGCTAGATG
ATGACGGAATGTTGAGTCAGAGCTTGGAAGAAATTTACGCGCAGCTACCAGAGAGCTTGGTGTTAGAGCTTGAGTTGGAG
CCAGAAGAATTACAAATTGCGCTACAACATTTACAGCAACTAGAACCACTCGGTGTCGGCGCACGTGATTTGCCCGAGTC
GTTAACATTGCAATTGCGAGTCTTGCCAGAAAGCACCATTCGAAATATTGCCATTCGATTGGTGAATGAAGGCCTCGATT
TATTGGCGCAACGGGATTACACTAGACTTAAACGTCAACTGCAGTGCGATGAAGTGGCACTCAAAGAAGCGCAAATATTA
ATTCGGCGTTTAACGCCAAGACCGGGTGCCAATTGGTCAGCAGAGACGATACGCTACGTGCTCCCTGATGTGATTGTGAC
CAAGGTGCGTGGGCAATGGCAGGTACGCTTAAATAGTGCGGCCCAGCCCAAGCTAAGAGTCAATGAATTGTATGCAAAGT
TACTACAAGGCGACTCAGCAGCAGCACTCAGTGGTAAGCTACAAGAAGCACGTTGGCTGATTAAAAGTGTTGAGCAACGA
GGCAGTACGATTTTACGTGTCGCAGAAGCAATCGTAATGCGACAAAAAGCATTTTTTGAGTATGGTGAAGTGGCAATGCG
GCCTTTAGTGCTGCGCGATATTGCCGATGAGCTTGAATTGCATGAATCGACGATTTCTCGAGTAACAACGCAAAAATACA
TGCTGACTCCGCGCGGTGTTTTTGAATACAAATATTTTTTTGGCAGTCATGTTGAAAGTGAAAGCGGTGGTGAAAACTCA
GCAACATCGATTAAGGCTTTGATCAAACAACTGGTACAGCAAGAAAATCAGCACAAGCCATTATCCGATAGTGCTATAAC
TGAAGAACTCGCACGGCAAGGTGTTGTGGTTGCGCGGCGCACCGTTGCTAAGTACAGGGAGGCATTAATGATTCCTCCCG
TTAATCTGCGCAAGTGCTTATAA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(101-291)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(303-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M8SRP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.372

100

0.576