Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   HRJ47_RS16635 Genome accession   NZ_CP054137
Coordinates   3410288..3411088 (-) Length   266 a.a.
NCBI ID   WP_203079963.1    Uniprot ID   -
Organism   Acinetobacter pittii strain JXA13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3405288..3416088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HRJ47_RS16615 (HRJ47_16605) - 3406353..3407051 (-) 699 WP_086400272.1 YfgM family protein -
  HRJ47_RS16620 (HRJ47_16610) hisS 3407080..3408372 (-) 1293 WP_086400273.1 histidine--tRNA ligase -
  HRJ47_RS16625 (HRJ47_16615) ispG 3408369..3409484 (-) 1116 WP_086400274.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  HRJ47_RS16630 (HRJ47_16620) - 3409506..3410297 (-) 792 WP_033849429.1 helix-turn-helix domain-containing protein -
  HRJ47_RS16635 (HRJ47_16625) pilF 3410288..3411088 (-) 801 WP_203079963.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  HRJ47_RS16640 (HRJ47_16630) rlmN 3411104..3412336 (-) 1233 WP_000093084.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  HRJ47_RS16645 (HRJ47_16635) ndk 3412460..3412891 (-) 432 WP_000963851.1 nucleoside-diphosphate kinase -
  HRJ47_RS16650 (HRJ47_16640) iscX 3413005..3413205 (-) 201 WP_004698764.1 Fe-S cluster assembly protein IscX -
  HRJ47_RS16655 (HRJ47_16645) - 3413305..3413856 (-) 552 WP_005066176.1 phosphatase PAP2 family protein -
  HRJ47_RS16660 (HRJ47_16650) - 3413881..3415170 (-) 1290 WP_081032067.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30044.34 Da        Isoelectric Point: 9.0211

>NTDB_id=451052 HRJ47_RS16635 WP_203079963.1 3410288..3411088(-) (pilF) [Acinetobacter pittii strain JXA13]
MSTPKLKIMLCMGVAVALLASGCQTSQTVKKDPEKAVKVRTQLAAEYIRSGDLDSAKRALDQALSVDSRDATANMMMGIL
LQQEGSKPNLEKAEHYFKRAISSEPDNAQAHNNYGTYLYQMERYNDAVEQFRIAGATLGYDQRYQALENLGRIYLKLGDV
ANAEKTFKQALLANRDSYISMLELAEIFYLQQQIPAATQMYEQYVRTVGQKNQGARALWIGVRVARANADKMGMQVLVNQ
LRALFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=451052 HRJ47_RS16635 WP_203079963.1 3410288..3411088(-) (pilF) [Acinetobacter pittii strain JXA13]
TTGAGTACTCCGAAGTTGAAGATCATGTTATGTATGGGGGTTGCTGTTGCACTTTTGGCAAGTGGTTGCCAGACTTCGCA
AACGGTTAAAAAGGACCCGGAAAAAGCAGTAAAAGTGCGTACGCAGCTTGCTGCCGAATACATTCGTTCTGGTGACTTGG
ACTCAGCAAAGCGAGCTCTTGATCAAGCTTTAAGTGTAGATAGCCGCGATGCTACTGCAAATATGATGATGGGTATTTTG
CTACAGCAAGAAGGTAGCAAACCCAATTTAGAAAAAGCAGAACATTATTTCAAACGAGCAATTTCATCTGAACCGGATAA
TGCTCAAGCACATAATAATTATGGTACTTATTTATATCAAATGGAGCGTTATAATGATGCGGTTGAGCAATTTCGTATTG
CTGGCGCAACATTAGGTTATGACCAACGCTATCAGGCATTAGAAAATCTGGGGCGTATTTATTTAAAATTGGGTGATGTT
GCGAATGCTGAAAAAACATTCAAACAAGCACTCCTTGCTAACCGCGACTCTTATATTTCAATGTTAGAGTTAGCGGAAAT
TTTTTATTTGCAGCAGCAGATTCCGGCTGCTACACAAATGTATGAGCAATATGTTCGTACAGTGGGGCAGAAAAATCAGG
GTGCAAGAGCACTTTGGATTGGTGTAAGAGTGGCTCGAGCCAATGCGGATAAAATGGGAATGCAAGTGTTAGTAAACCAG
TTACGTGCCTTGTTCCCGGAAAGTCCAGAATATCAACGTTATTTGCAATTACAGTATAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(89-134)

Tetratricopeptide repeat (TPR_16)

(149-206)

Tetratricopeptide repeat (TPR_16)

(43-79)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

97.744

100

0.977
  pilF Acinetobacter baylyi ADP1

75.385

97.744

0.737