Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   A8O29_RS03095 Genome accession   NZ_CP054058
Coordinates   488708..490141 (-) Length   477 a.a.
NCBI ID   WP_125352475.1    Uniprot ID   -
Organism   Scandinavium goeteborgense strain CCUG 66741     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 483708..495141
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8O29_RS03065 (A8O29_003065) elbB 485261..485914 (+) 654 WP_125352481.1 isoprenoid biosynthesis glyoxalase ElbB -
  A8O29_RS03070 (A8O29_003070) mtgA 485911..486636 (+) 726 WP_125352479.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  A8O29_RS03075 (A8O29_003075) npr 486692..486964 (-) 273 WP_110510588.1 PTS phosphocarrier protein NPr -
  A8O29_RS03080 (A8O29_003080) rapZ 486961..487815 (-) 855 WP_110510587.1 RNase adapter RapZ -
  A8O29_RS03085 (A8O29_003085) ptsN 487849..488340 (-) 492 WP_125352477.1 PTS IIA-like nitrogen regulatory protein PtsN -
  A8O29_RS03090 (A8O29_003090) hpf 488398..488685 (-) 288 WP_110510585.1 ribosome hibernation promoting factor -
  A8O29_RS03095 (A8O29_003095) rpoN/rpoN1 488708..490141 (-) 1434 WP_125352475.1 RNA polymerase factor sigma-54 Machinery gene
  A8O29_RS03100 (A8O29_003100) lptB 490189..490914 (-) 726 WP_110510583.1 LPS export ABC transporter ATP-binding protein -
  A8O29_RS03105 (A8O29_003105) lptA 490921..491478 (-) 558 WP_110510582.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  A8O29_RS03110 (A8O29_003110) lptC 491462..492022 (-) 561 WP_110510581.1 LPS export ABC transporter periplasmic protein LptC -
  A8O29_RS03115 (A8O29_003115) kdsC 492019..492585 (-) 567 WP_125352473.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  A8O29_RS03120 (A8O29_003120) kdsD 492600..493586 (-) 987 WP_125352471.1 arabinose-5-phosphate isomerase KdsD -
  A8O29_RS03125 (A8O29_003125) - 493601..494578 (-) 978 WP_125352469.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 53758.59 Da        Isoelectric Point: 4.3626

>NTDB_id=450464 A8O29_RS03095 WP_125352475.1 488708..490141(-) (rpoN/rpoN1) [Scandinavium goeteborgense strain CCUG 66741]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESNPLLEQTDLHEEVDSQEETDKDEFDTVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTGTDYQDDELPVYQGETTQSLQDYLMWQVGLTPFSDTDRAIATSIVNAVDDTGYLTASLEE
ILESIGDEELELDEVEAVLKRIQRFDPIGVAAKDLRDCLLIQLSQFAKETPWLTEAKTIISDHLSLLANHDFRSLMRVTR
LKEDVLKEAVSLIQSLDPRPGQSIQTGEPEYVIPDVLVRKISGRWAVELNSDSIPRLQINQHYAGMCSTTRNDADSQFIR
SNLQEAKWLIKSLESRNDTLLRVSRCIVEQQQAFFDQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLVAAENPAKPLSDSKLTTMLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=450464 A8O29_RS03095 WP_125352475.1 488708..490141(-) (rpoN/rpoN1) [Scandinavium goeteborgense strain CCUG 66741]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAGCTCGCCATGACGCCGCAACTGCAACAGGCCATTCGTCTGTTGCA
GCTTTCTACGCTCGAGCTGCAACAGGAACTTCAGCAAGCGCTGGAGAGTAATCCCCTGCTTGAGCAAACCGACCTTCACG
AGGAAGTGGATTCACAAGAAGAAACTGACAAAGACGAATTTGATACCGTTGATGCTCTCGAGCAGAAAGAGATGCCCGAT
GAGCTTCCGCTCGACGCCAGCTGGGATGAAATCTACACCGCAGGCACGCCGTCCGGTACCGGCACTGATTATCAGGATGA
CGAACTGCCGGTCTACCAGGGCGAAACGACACAGTCACTTCAGGACTATCTGATGTGGCAGGTGGGGCTGACACCGTTTT
CCGATACCGACCGCGCCATAGCCACATCGATCGTGAACGCGGTTGACGACACCGGCTATCTCACCGCGTCGCTGGAAGAG
ATCCTCGAAAGCATCGGCGACGAAGAACTCGAACTCGATGAGGTCGAAGCAGTTCTGAAACGCATCCAGCGTTTTGATCC
GATCGGCGTGGCCGCGAAAGATCTGCGCGACTGCCTGTTGATCCAGCTCTCCCAGTTTGCCAAAGAGACACCGTGGCTGA
CGGAAGCCAAAACGATTATCAGCGATCATCTCTCCCTGCTGGCCAATCATGATTTCCGCTCCCTGATGCGTGTCACTCGC
CTCAAAGAAGATGTGCTGAAAGAAGCCGTGTCATTGATTCAGTCTCTGGATCCGCGTCCGGGCCAGTCGATTCAGACCGG
CGAACCGGAATACGTCATCCCCGATGTGCTGGTGCGTAAAATCAGCGGCCGTTGGGCAGTTGAACTCAATTCCGACAGTA
TCCCGCGTCTGCAAATCAATCAGCATTACGCCGGTATGTGCAGCACTACGCGCAACGATGCCGACAGCCAATTTATTCGC
AGCAATCTGCAGGAAGCTAAATGGCTGATTAAAAGCCTGGAAAGCCGCAATGACACGCTGCTGCGGGTGAGCCGCTGCAT
CGTAGAACAGCAGCAGGCGTTCTTTGATCAGGGCGAAGAGTTCATGAAGCCGATGGTCCTGGCGGATATCGCTCAGGCCG
TCGAAATGCATGAATCAACGATTTCACGCGTTACCACGCAAAAGTATCTGCACAGCCCACGGGGCATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCATGTGAATACTGAAGGCGGCGGCGAAGCGTCGTCCACGGCTATTCGCGCACTGGTGAAGAAGTT
AGTTGCCGCGGAAAATCCCGCGAAACCACTGAGTGACAGTAAGCTGACTACCATGCTGTCCGAACAGGGTATCATGGTGG
CACGCCGCACCGTTGCAAAGTACCGAGAGTCTTTATCCATCCCGCCGTCGAACCAGCGCAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.941

100

0.486