Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   I5367_RS08610 Genome accession   NZ_CP065539
Coordinates   1768126..1768560 (+) Length   144 a.a.
NCBI ID   WP_012117601.1    Uniprot ID   A7Z4Z1
Organism   Bacillus amyloliquefaciens strain DGL1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1763126..1773560
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5367_RS08605 (I5367_08605) - 1766626..1767837 (-) 1212 WP_012117600.1 cytochrome P450 family protein -
  I5367_RS08610 (I5367_08610) nucA/comI 1768126..1768560 (+) 435 WP_012117601.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  I5367_RS08615 (I5367_08615) - 1768620..1769375 (+) 756 WP_023356903.1 YoaK family protein -
  I5367_RS08620 (I5367_08620) - 1769409..1769771 (-) 363 WP_007410383.1 hypothetical protein -
  I5367_RS08625 (I5367_08625) - 1769964..1771292 (-) 1329 WP_012117603.1 S8 family peptidase -
  I5367_RS08630 (I5367_08630) - 1771471..1771704 (+) 234 WP_015239897.1 hypothetical protein -
  I5367_RS08635 (I5367_08635) - 1771960..1772667 (+) 708 WP_012117604.1 poly-gamma-glutamate hydrolase family protein -
  I5367_RS08640 (I5367_08640) - 1772727..1773179 (+) 453 WP_012117605.1 OsmC family protein -
  I5367_RS08645 (I5367_08645) - 1773193..1773546 (-) 354 WP_007410379.1 DMT family transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15562.48 Da        Isoelectric Point: 7.2418

>NTDB_id=449996 I5367_RS08610 WP_012117601.1 1768126..1768560(+) (nucA/comI) [Bacillus amyloliquefaciens strain DGL1]
MNAFMKWAASLLLVISLQFGFTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=449996 I5367_RS08610 WP_012117601.1 1768126..1768560(+) (nucA/comI) [Bacillus amyloliquefaciens strain DGL1]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCTTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAAAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7Z4Z1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

58.974

81.25

0.479