Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilH   Type   Machinery gene
Locus tag   I5367_RS08170 Genome accession   NZ_CP065539
Coordinates   1614017..1614379 (+) Length   120 a.a.
NCBI ID   WP_003154238.1    Uniprot ID   A0A150FA43
Organism   Bacillus amyloliquefaciens strain DGL1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1609017..1619379
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5367_RS08140 (I5367_08140) flgD 1609920..1610360 (+) 441 WP_012117554.1 flagellar hook assembly protein FlgD -
  I5367_RS08145 (I5367_08145) flgG 1610385..1611161 (+) 777 WP_007409783.1 flagellar basal body rod protein FlgG -
  I5367_RS08150 (I5367_08150) - 1611201..1611416 (+) 216 WP_003154243.1 flagellar FlbD family protein -
  I5367_RS08155 (I5367_08155) fliL 1611413..1611832 (+) 420 WP_003154241.1 flagellar basal body-associated protein FliL -
  I5367_RS08160 (I5367_08160) fliM 1611866..1612864 (+) 999 WP_003154240.1 flagellar motor switch protein FliM -
  I5367_RS08165 (I5367_08165) fliY 1612854..1613990 (+) 1137 WP_012117555.1 flagellar motor switch phosphatase FliY -
  I5367_RS08170 (I5367_08170) pilH 1614017..1614379 (+) 363 WP_003154238.1 chemotaxis protein CheY Machinery gene
  I5367_RS08175 (I5367_08175) fliZ 1614394..1615047 (+) 654 WP_003154237.1 flagella biosynthesis regulatory protein FliZ -
  I5367_RS08180 (I5367_08180) fliP 1615040..1615705 (+) 666 WP_012117556.1 flagellar type III secretion system pore protein FliP -
  I5367_RS08185 (I5367_08185) fliQ 1615720..1615989 (+) 270 WP_003154234.1 flagellar biosynthesis protein FliQ -
  I5367_RS08190 (I5367_08190) fliR 1615996..1616775 (+) 780 WP_012117557.1 flagellar biosynthetic protein FliR -
  I5367_RS08195 (I5367_08195) flhB 1616775..1617854 (+) 1080 WP_007409787.1 flagellar biosynthesis protein FlhB -

Sequence

Protein


Download         Length: 120 a.a.        Molecular weight: 13209.53 Da        Isoelectric Point: 4.6805

>NTDB_id=449989 I5367_RS08170 WP_003154238.1 1614017..1614379(+) (pilH) [Bacillus amyloliquefaciens strain DGL1]
MAHRILIVDDAAFMRMMIKDILVKNGFDVVAEASDGAQAVEKFKEHSPDLVTMDITMPEMDGITALKEIKQIDPQAKIIM
CSAMGQQSMVIDAIQAGAKDFIVKPFQADRVLEAINKTLS

Nucleotide


Download         Length: 363 bp        

>NTDB_id=449989 I5367_RS08170 WP_003154238.1 1614017..1614379(+) (pilH) [Bacillus amyloliquefaciens strain DGL1]
ATGGCACACAGAATTTTAATTGTAGACGATGCAGCATTTATGAGAATGATGATCAAGGATATTTTAGTGAAGAACGGTTT
TGACGTTGTAGCTGAAGCTTCAGACGGAGCGCAGGCAGTCGAAAAATTCAAAGAGCATTCCCCCGACCTTGTGACAATGG
ATATTACAATGCCTGAAATGGACGGGATTACAGCTTTAAAGGAAATTAAACAAATCGATCCGCAGGCGAAAATCATTATG
TGTTCGGCTATGGGCCAGCAGTCAATGGTTATTGACGCGATTCAAGCCGGCGCAAAAGACTTTATCGTGAAACCGTTCCA
AGCGGACCGCGTGCTTGAAGCTATTAATAAAACATTAAGCTAA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(5-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A150FA43

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilH Synechocystis sp. PCC 6803

38.983

98.333

0.383