Detailed information NTG0449933

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: I5367_RS01535
• Genome accession: NZ_CP065539
• Coordinates: 275098..276219 (+)
• Length: 373 a.a.
• NCBI ID: WP_011996281.1
• Uniprot ID: A7Z131
• Organism: Bacillus amyloliquefaciens strain DGL1
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 270098..281219

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I5367_RS01515 (I5367_01515)	-	270407..271402 (-)	996	WP_007409036.1	DUF5105 domain-containing protein	-
I5367_RS01520 (I5367_01520)	-	271529..272929 (+)	1401	WP_041482174.1	YcdB/YcdC domain-containing protein	-
I5367_RS01525 (I5367_01525)	-	273130..274488 (+)	1359	WP_023356694.1	YcdB/YcdC domain-containing protein	-
I5367_RS01530 (I5367_01530)	-	274485..274988 (-)	504	WP_011996280.1	M15 family metallopeptidase	-
I5367_RS01535 (I5367_01535)	rapF	275098..276219 (+)	1122	WP_011996281.1	Rap family tetratricopeptide repeat protein	Regulator
I5367_RS01540 (I5367_01540)	-	276335..277114 (+)	780	WP_011996282.1	glucose 1-dehydrogenase	-
I5367_RS01545 (I5367_01545)	-	277198..278877 (+)	1680	WP_011996283.1	glycoside hydrolase family 13 protein	-
I5367_RS01550 (I5367_01550)	-	279063..280022 (+)	960	WP_011996284.1	metal ABC transporter substrate-binding protein	-
I5367_RS01555 (I5367_01555)	-	280077..280772 (+)	696	WP_011996285.1	metal ABC transporter ATP-binding protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44317.53 Da
• Isoelectric Point: 5.0806

>NTDB_id=449933 I5367_RS01535 WP_011996281.1 275098..276219(+) (rapF) [Bacillus amyloliquefaciens strain DGL1]
MKSKIPSEEVAVKMNEWYKLIRAFEADQAEALKREIQYELEDMEENQDLLLYFSLMEFRHRIMLDKLKPMKDGDCQPPFS
EMLTEIEMNQQKLTGLLEYYFYYFRGMYEFKQKNFISAISHYKRAEEKLEYVEDDIEKAEFLYKISEVYYHIKQTFFSMN
YAGQALDIYKKYELYGMRRVQCKFIIAGNLMDAQQHKKALEYLECALEHAERLDDQYMIAAAFYNIGHCMYSLGDFCKAA
EDFERAAAGFEAHSFRQVIQAVFSLTHIYFKTEKYSEAAASCARGMRSAAEWEDEIYMTKFRLIKELYQGEGGQDSLKEY
FDILESKELLADTEELLQDAAHFFHKRENYKAASYFFSRLVGVQKRLQMNNYA

Nucleotide

• Length: 1122 bp

>NTDB_id=449933 I5367_RS01535 WP_011996281.1 275098..276219(+) (rapF) [Bacillus amyloliquefaciens strain DGL1]
ATGAAATCCAAGATTCCATCTGAAGAAGTGGCCGTAAAGATGAATGAATGGTATAAGCTGATCCGCGCGTTTGAAGCCGA
TCAAGCTGAAGCGCTGAAGCGGGAGATTCAATATGAACTCGAAGATATGGAAGAGAACCAGGATTTATTGTTGTATTTTT
CATTGATGGAATTTCGGCACCGCATCATGCTGGATAAATTAAAACCCATGAAAGACGGCGACTGCCAGCCTCCGTTCTCT
GAAATGCTCACTGAGATTGAGATGAACCAGCAAAAGCTGACCGGCTTACTGGAATATTATTTTTACTATTTCAGGGGAAT
GTATGAATTTAAGCAGAAAAACTTCATCTCAGCGATTTCTCATTATAAGCGGGCTGAGGAAAAGCTTGAATATGTGGAAG
ATGACATAGAGAAAGCCGAGTTTCTGTATAAAATTTCTGAGGTGTACTATCATATCAAGCAGACTTTCTTTTCGATGAAT
TACGCGGGGCAGGCGCTTGATATTTACAAGAAATATGAATTATACGGAATGAGAAGGGTGCAGTGCAAATTTATCATTGC
CGGAAATCTGATGGACGCCCAGCAGCATAAAAAAGCGCTGGAATACCTCGAATGCGCCTTGGAACACGCGGAGCGGCTTG
ATGATCAATATATGATTGCTGCGGCCTTTTATAATATCGGGCACTGTATGTACAGTCTGGGTGACTTTTGCAAGGCGGCG
GAGGATTTTGAACGGGCGGCTGCCGGATTTGAAGCACACAGCTTCCGGCAGGTCATCCAGGCTGTTTTTTCACTCACGCA
TATCTACTTTAAAACGGAAAAATACAGTGAAGCCGCCGCCAGCTGCGCGCGGGGCATGAGAAGCGCGGCGGAGTGGGAAG
ATGAGATCTATATGACGAAATTCCGTCTGATTAAAGAGCTTTATCAGGGGGAAGGCGGACAGGATTCGCTCAAAGAGTAT
TTTGACATTCTGGAATCAAAAGAGCTGTTGGCTGACACAGAGGAACTGCTTCAGGACGCGGCTCATTTCTTTCATAAACG
GGAGAACTACAAAGCGGCATCTTATTTTTTCAGCAGGCTTGTCGGCGTGCAAAAAAGGCTGCAAATGAACAACTACGCAT
AA

Domains

Predicted by InterProScan.

• Tetratricopeptide repeat (TPR_12) (187-251)

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

3D structure

• AlphaFold DB: A7Z131

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.588	99.464	0.424
rapC	Bacillus subtilis subsp. subtilis str. 168	40.054	99.732	0.399