Detailed information NTG0449824

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: FOC75_RS03800
• Genome accession: NZ_CP053991
• Coordinates: 656266..659988 (+)
• Length: 1240 a.a.
• NCBI ID: WP_000572289.1
• Uniprot ID: B7JDU4
• Organism: Bacillus cereus strain FDAARGOS_781
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 651266..664988

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FOC75_RS03785 (FOC75_03785)	-	651424..652017 (+)	594	WP_000347516.1	TVP38/TMEM64 family protein	-
FOC75_RS03790 (FOC75_03790)	lepB	652074..652637 (+)	564	WP_000751894.1	signal peptidase I	-
FOC75_RS03795 (FOC75_03795)	addB	652754..656269 (+)	3516	WP_000058558.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
FOC75_RS03800 (FOC75_03800)	addA	656266..659988 (+)	3723	WP_000572289.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
FOC75_RS03805 (FOC75_03805)	-	660001..660288 (+)	288	WP_000255719.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
FOC75_RS03810 (FOC75_03810)	gerPF	660326..660541 (-)	216	WP_001141566.1	spore germination protein GerPF	-
FOC75_RS03815 (FOC75_03815)	-	660584..660970 (-)	387	WP_000902341.1	spore germination protein GerPE	-
FOC75_RS03820 (FOC75_03820)	gerPD	660986..661180 (-)	195	WP_001052802.1	spore germination protein GerPD	-
FOC75_RS03825 (FOC75_03825)	gerPC	661187..661801 (-)	615	WP_001070767.1	spore germination protein GerPC	-
FOC75_RS03830 (FOC75_03830)	gerPB	661869..662075 (-)	207	WP_001012512.1	spore germination protein GerPB	-
FOC75_RS03835 (FOC75_03835)	gerPA	662090..662311 (-)	222	WP_001111188.1	spore germination protein GerPA	-
FOC75_RS03840 (FOC75_03840)	-	662408..662587 (-)	180	WP_000462851.1	aspartyl-phosphate phosphatase Spo0E family protein	-
FOC75_RS03845 (FOC75_03845)	-	662835..663734 (+)	900	WP_001241727.1	fumarylacetoacetate hydrolase family protein	-
FOC75_RS03850 (FOC75_03850)	-	663770..664054 (-)	285	WP_000925338.1	hypothetical protein	-

Sequence

Protein

• Length: 1240 a.a.
• Molecular weight: 142649.00 Da
• Isoelectric Point: 4.8851

>NTDB_id=449824 FOC75_RS03800 WP_000572289.1 656266..659988(+) (addA) [Bacillus cereus strain FDAARGOS_781]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHVRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRIETLQADLALFGTLSAAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDIVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEVIDGEEGAEVEKAQLEARLMA
QRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDNP
MQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEKELHDKLEWFYNLLQGWREFARQQSLSDLI
WKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTIH
KSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTRA
KEKLILIGTVKDATKEMEKWLDAREHSEWLLPDHVRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTSWKV
EVVDGNTLLAPEPVQEEKQELLEALREKKAVPLESERKEEVYDRLMWKYRYGEATSHRAKQSVTEIKRNYQSEEGSDNAF
IKKLRAPIRTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAVEKVISFFDSD
LGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPILE
ERYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVIKVEE

Nucleotide

• Length: 3723 bp

>NTDB_id=449824 FOC75_RS03800 WP_000572289.1 656266..659988(+) (addA) [Bacillus cereus strain FDAARGOS_781]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAGTGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCGGCAGCAGCAGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACAAATGCAGCAGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGAACCAGGATCTCAGCACGTAAGAAAGCAACTGAGCTTATTAAATAAAGC
TTCCATTTCAACGATCCATTCATTTTGTTTACAAGTTATTAGAGGATATTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATTGCGAATCAAACAGAAAATGAATTATTAAAAGAAGAAGTGTTAGATGACATATTAGAAGAAGAGTATGGAATAGAA
GATAATACGATATTCTTTGAACTCGTTGATCGTTATACGAGCGACCGTAGTGATGATGATTTACAACGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATATGATGTCGAAGGAA
AGACAATTGAAGATTTAGTGTACGCGTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAAGCAACTGAGCTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCATTGAAACGCTGCAAGCAGATTTAGCTTTATT
TGGAACGTTATCAGCAGCTGCTCGTGAATCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGCATTAAGAAAAGCGATTATAACGAGGATATTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCAAAAGATGAAGTG
AAGAAATTACAAGAAGAGCTATTTAGCCGCAGGCCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAGCTCGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGCATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAGATGGTGAAATGAAGCCATCAGCAGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCAATTATTAAATTCGT
AACGAAAGATTCCGAGAGTGAAGGAAACTTATTCATGGTTGGTGACGTGAAGCAGTCGATTTATCGTTTCCGACTAGCAG
AACCAGGATTATTCTTAGGAAAGTATAAACGTTTCACACAAGAAGGATTAGGCGGCGGAATGAAAATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCGGGTACAAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
TGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCAGCTGAACTATTGTGCA
TTCAGCAAACAGAAGAAGTAATAGACGGTGAAGAAGGTGCCGAAGTAGAAAAGGCACAGCTTGAAGCACGTCTTATGGCA
CAGCGCATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATACCG
TGACTTCGTTATTTTACTTCGCTCGATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCAGTAT
ACGCTGATCTTGCCACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAATCCG
ATGCAAGATATTCCGCTTGCAGCAGTACTTCGTTCCCCAATCGTTGGATTAAATGATGAAGAACTTGCAACGCTTCGTGC
TCACGGGAAGAAAGGCTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGAGCACCGCTTGAAGAAGAAAAAGAACTAC
ATGATAAATTAGAATGGTTCTATAACTTACTGCAAGGATGGCGTGAATTCGCACGCCAACAGTCTCTTTCTGATTTAATT
TGGAAAGTGTACGGTGAGACAGGTTATTATGACTTTGTCGGTGGTTTACCAGCTGGAAAGCAAAGGCAAGCAAACCTGCG
TGTACTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGACTATTCCGCTTCTTACGCTTTATTGAGCGTA
TTTTAGAACGCGGTGATGATATGGGTACGGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGCATTATGACAATTCAT
AAAAGTAAAGGACTTGAGTTCCCAGTCGTATTCGTCGCTGGACTTGGTCGTCGTTTTAATACACAAGACTTAATGAAACG
TTTCTTACTTCATAAAGACTTCGGTTTCGGTTCGCAATTTATCGATCCGCGTAAACGAATTAAATATACGACATTATCAC
AACTTGCAATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTCTTATACGTAGCGTTAACACGGGCA
AAAGAGAAGTTAATTTTAATTGGAACGGTTAAGGATGCAACTAAGGAAATGGAAAAATGGCTGGATGCGAGGGAGCATAG
TGAATGGTTATTACCAGATCACGTACGTGCCGGAGCATCTTGTTATTTAGACTGGATTGCACCTTCCTTATATAGACACC
GTGATAGTGAAATGCTTCTTGAATTAGGGCAAGGAAGTATTCCAGATGAAATTTATGGGTATGACACTAGCTGGAAAGTA
GAAGTTGTTGACGGGAACACGTTACTTGCGCCAGAACCCGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCGTGA
GAAAAAGGCTGTTCCGTTAGAGAGTGAACGGAAAGAAGAAGTGTACGATAGATTAATGTGGAAGTACAGATATGGAGAAG
CGACATCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCCTTT
ATTAAAAAACTACGTGCACCAATTAGAACACGTCCTCGTTTCATGGAAAAAAAGGGGTTAACGTACGCAGAGCGCGGAAC
AGCAGTCCATGCTGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACAGTTGAAGTTCTTCAAGAGCAAATTGCTGGAA
TGGTAAATAAAGAATTATTAACATTTGAACAAGCAGAAGAAATAGCAGTTGAAAAAGTGATTTCATTCTTTGACAGTGAC
CTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGCGTA
TCAAGATTGGCAAGGGAAGAGCGGGGAATCAATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATGGTA
TTACTTTAATCGACTTTAAAACGGATACGATTGAAGGGAAGTTCCCGGGAGGATTCGAACAAGCGAAACCAATTTTAGAA
GAGCGATATAAAGTGCAGCTTTCGTTATATGCAAAGGCACTTGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATGTTT
ATACTTCTTTGATGGTAATCATGTTATAAAAGTTGAGGAATAG

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1058-1231)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-889)

3D structure

• AlphaFold DB: B7JDU4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.71	100	0.537