Detailed information NTG0449630

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: FOC89_RS19525
• Genome accession: NZ_CP053980
• Coordinates: 3172972..3176694 (+)
• Length: 1240 a.a.
• NCBI ID: WP_000572265.1
• Uniprot ID: -
• Organism: Bacillus thuringiensis strain FDAARGOS_795
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 3167972..3181694

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FOC89_RS19510 (FOC89_19505)	-	3168130..3168723 (+)	594	WP_000347516.1	TVP38/TMEM64 family protein	-
FOC89_RS19515 (FOC89_19510)	lepB	3168780..3169343 (+)	564	WP_000751894.1	signal peptidase I	-
FOC89_RS19520 (FOC89_19515)	addB	3169460..3172975 (+)	3516	WP_000058572.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
FOC89_RS19525 (FOC89_19520)	addA	3172972..3176694 (+)	3723	WP_000572265.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
FOC89_RS19530 (FOC89_19525)	-	3176707..3176994 (+)	288	WP_000718626.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
FOC89_RS19535 (FOC89_19530)	gerPF	3177032..3177247 (-)	216	WP_001141566.1	spore germination protein GerPF	-
FOC89_RS19540 (FOC89_19535)	-	3177290..3177676 (-)	387	WP_000902341.1	spore germination protein GerPE	-
FOC89_RS19545 (FOC89_19540)	gerPD	3177692..3177886 (-)	195	WP_001052802.1	spore germination protein GerPD	-
FOC89_RS19550 (FOC89_19545)	gerPC	3177893..3178507 (-)	615	WP_001070767.1	spore germination protein GerPC	-
FOC89_RS19555 (FOC89_19550)	gerPB	3178575..3178781 (-)	207	WP_001012512.1	spore germination protein GerPB	-
FOC89_RS19560 (FOC89_19555)	gerPA	3178796..3179017 (-)	222	WP_001111188.1	spore germination protein GerPA	-
FOC89_RS19565 (FOC89_19560)	-	3179114..3179293 (-)	180	WP_000462851.1	aspartyl-phosphate phosphatase Spo0E family protein	-
FOC89_RS19570 (FOC89_19565)	-	3179541..3180440 (+)	900	WP_001241727.1	fumarylacetoacetate hydrolase family protein	-
FOC89_RS19575 (FOC89_19570)	-	3180481..3180765 (-)	285	WP_000925338.1	hypothetical protein	-

Sequence

Protein

• Length: 1240 a.a.
• Molecular weight: 142603.02 Da
• Isoelectric Point: 4.8661

>NTDB_id=449630 FOC89_RS19525 WP_000572265.1 3172972..3176694(+) (addA) [Bacillus thuringiensis strain FDAARGOS_795]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNMIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADLALLGTLSAAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDIVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEVIDGEEGAEVEKAQLEARLMA
QRIKAMVDSGYEVYDRKTDSMRPVKYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDNP
MQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSTFLKGAPLEEEKELHDKLEWFYNLLQGWREFARQQSLSDLI
WKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTIH
KSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTRA
KEKLILIGTVKDATKEMEKWLDAREHSEWLLPDHVRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTSWKV
EVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKDEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNAF
IKKLRAPIQTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAVEKVISFFDSD
LGKRILAAKSVEREVPFTMMLAAEEAYQDWQGKSGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPILE
ERYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVIKVEE

Nucleotide

• Length: 3723 bp

>NTDB_id=449630 FOC89_RS19525 WP_000572265.1 3172972..3176694(+) (addA) [Bacillus thuringiensis strain FDAARGOS_795]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAATGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCGGCAGCAGCTGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATAAATG
AAGAAAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGAGAGGCTTTAGAAAAAGTATTAATTGATGAGCCTGGCTCTCAGCACATCCGAAAGCAACTGAGCTTATTAAATAAAGC
TTCCATTTCAACGATCCATTCATTTTGTTTACAAGTTATTAGAGGATACTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATTGCGAATCAAACCGAAAATGAATTATTAAAAGAAGAAGTGTTAGATGACATATTAGAAGAAGAGTATGGAATAGAA
GATAATATGATATTCTTTGAACTCGTTGATCGTTATACGAGCGACCGTAGTGATGATGATTTACAACGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATATGACGTCGAAGGAA
AGACAATTGAAGATTTAGTGTACGCCTCTTACTTATTAGAAGATGTAAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAAGCAACTGAGCTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCGTTGAAACGCTGCAAGCAGATTTAGCTTTACT
TGGAACGTTATCAGCAGCTGCTCGTGAATCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGCATTAAGAAAAGCGATTATAACGAGGATATTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCAAAAGATGAAGTG
AAGAAATTACAAGAAGAGCTATTTAGCCGCAGGCCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAGCTCGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGCATGGTTGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAGATGGTGAAATGAAGCCATCAGCAGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCAATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTATTCATGGTTGGTGACGTGAAGCAGTCGATTTATCGTTTCCGACTAGCAG
AACCAGGATTATTCTTAGGAAAGTATAAACGTTTCACACAAGAAGGATTAGGCGGCGGAATGAAAATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCGGGTACAAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
TGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCAGCTGAACTATTGTGCA
TTCAACAAACAGAAGAAGTAATAGACGGTGAAGAAGGTGCGGAAGTAGAAAAGGCACAGCTTGAAGCACGTCTTATGGCG
CAGCGCATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTAAAATACCG
TGACTTCGTTATTTTACTTCGCTCGATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCAGTAT
ACGCTGACCTTGCCACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAATCCG
ATGCAAGATATTCCGCTTGCAGCAGTACTTCGTTCCCCAATCGTTGGATTAAATGATGAAGAACTTGCAACGCTTCGTGC
TCACGGGAAGAAAGGCTCGTTTTATGAAGTAATGAGCACATTCTTAAAAGGAGCACCGCTTGAAGAAGAAAAAGAACTAC
ATGATAAATTAGAATGGTTCTATAACTTACTGCAAGGATGGCGTGAATTCGCACGCCAACAGTCTCTTTCTGATTTAATT
TGGAAAGTGTACGGTGAGACAGGTTATTATGACTTTGTCGGTGGTTTACCAGCTGGAAAGCAAAGGCAAGCAAACCTGCG
TGTACTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGACTATTCCGCTTCTTACGCTTTATTGAGCGTA
TTTTAGAACGCGGTGATGATATGGGTACGGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGCATTATGACAATTCAT
AAAAGTAAAGGACTTGAGTTCCCAGTCGTATTCGTCGCTGGACTTGGTCGTCGTTTTAATACGCAAGACTTAATGAAACG
TTTCTTACTTCATAAAGACTTCGGTTTCGGTTCGCAATTTATCGATCCGCGTAAACGAATTAAATATACGACATTATCAC
AACTTGCAATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTCTTATACGTAGCGTTAACACGGGCA
AAAGAGAAGTTAATTTTAATTGGAACGGTTAAGGATGCAACTAAGGAAATGGAAAAATGGCTGGATGCGAGGGAGCATAG
TGAATGGTTATTACCAGATCACGTACGTGCCGGAGCATCTTGTTATTTAGACTGGATTGCACCTTCCTTATATAGACACC
GTGATAGTGAAATGCTTCTTGAATTAGGGCAAGGAAGTATTCCAGATGAAATTTATGGGTATGACACTAGCTGGAAAGTA
GAAGTTGTTGACGGGAACACGTTACTTGCGCCAGAACCCGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCGTGA
GAAAAAAGCTGTTCCCCTGCAAAGTGAACGAAAAGATGAAGTGTACGACAGGTTAATGTGGAAGTACGGATATGAGGAAG
CGACATCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCTTTT
ATTAAAAAATTACGTGCACCAATTCAAACACGTCCTCGTTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCGAGGAAC
AGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACAGTTGAAGTTCTTCAAGAGCAAATTGCTGGAA
TGGTAAATAAAGAATTATTAACATTTGAACAAGCAGAAGAAATAGCAGTTGAAAAAGTGATTTCATTCTTTGACAGTGAC
CTAGGTAAAAGGATATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGCGTA
TCAAGATTGGCAAGGGAAGAGCGGGGAATCAATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATGGTA
TTACTTTAATCGACTTTAAAACGGATACGATTGAAGGGAAGTTCCCGGGAGGATTCGAACAAGCGAAACCAATTTTAGAA
GAGCGATATAAAGTGCAGCTTTCGTTATATGCAAAGGCACTTGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATGTTT
ATACTTCTTTGATGGTAATCATGTTATAAAAGTTGAGGAATAG

Domains

Predicted by InterproScan.

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1059-1231)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-889)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.629	100	0.536