Detailed information NTG0449460

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: FOC76_RS26245
• Genome accession: NZ_CP053955
• Coordinates: 5116437..5120162 (-)
• Length: 1241 a.a.
• NCBI ID: WP_000572279.1
• Uniprot ID: -
• Organism: Bacillus tropicus strain FDAARGOS_782
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 5111437..5125162

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FOC76_RS26195 (FOC76_26195)	-	5112298..5112576 (+)	279	WP_000925340.1	hypothetical protein	-
FOC76_RS26200 (FOC76_26200)	-	5112622..5113521 (-)	900	WP_001241721.1	fumarylacetoacetate hydrolase family protein	-
FOC76_RS26205 (FOC76_26205)	-	5113769..5113948 (+)	180	WP_000462840.1	aspartyl-phosphate phosphatase Spo0E family protein	-
FOC76_RS26210 (FOC76_26210)	gerPA	5114045..5114266 (+)	222	WP_001111187.1	spore germination protein GerPA	-
FOC76_RS26215 (FOC76_26215)	gerPB	5114281..5114487 (+)	207	WP_001012508.1	spore germination protein GerPB	-
FOC76_RS26220 (FOC76_26220)	gerPC	5114556..5115170 (+)	615	WP_001070751.1	spore germination protein GerPC	-
FOC76_RS26225 (FOC76_26225)	gerPD	5115177..5115371 (+)	195	WP_001102341.1	spore germination protein GerPD	-
FOC76_RS26230 (FOC76_26230)	-	5115387..5115773 (+)	387	WP_000902316.1	spore germination protein GerPE	-
FOC76_RS26235 (FOC76_26235)	gerPF	5115816..5116031 (+)	216	WP_001141566.1	spore germination protein GerPF	-
FOC76_RS26240 (FOC76_26240)	-	5116137..5116424 (-)	288	WP_000179953.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
FOC76_RS26245 (FOC76_26245)	addA	5116437..5120162 (-)	3726	WP_000572279.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
FOC76_RS26250 (FOC76_26250)	addB	5120159..5123674 (-)	3516	WP_000058546.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
FOC76_RS26255 (FOC76_26255)	-	5123861..5124127 (-)	267	WP_001084250.1	DUF4176 domain-containing protein	-
FOC76_RS26260 (FOC76_26260)	-	5124124..5124831 (-)	708	WP_000362191.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142740.13 Da
• Isoelectric Point: 4.8843

>NTDB_id=449460 FOC76_RS26245 WP_000572279.1 5116437..5120162(-) (addA) [Bacillus tropicus strain FDAARGOS_782]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRVETLQADLVLLGTLSSAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDVVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFQDMHPVLEKLVQLVKVFTERFQTMKRDKGMVDFTDLEHFCLQILSEQSENGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGATYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEKELHDKLEWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDANKEMEKWLDAREHSEWLLPDHIRAGASCYLDWIAPSLYRHRDSEILLELGQGSIPDEIYGYSASWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKEEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIRTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITIEVLQEQIAGMVNKELLTFEQAEEIAIEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGKSGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EERYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVVKIEE

Nucleotide

• Length: 3726 bp

>NTDB_id=449460 FOC76_RS26245 WP_000572279.1 5116437..5120162(-) (addA) [Bacillus tropicus strain FDAARGOS_782]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAGTGGACAGATGACCAGTGGAAAGCGGTTGTAGCGAACGGACG
TGATATTTTAGTCGCGGCAGCAGCAGGATCAGGGAAAACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATTAATG
AAGAAAACCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCGTTAGAAAAAGTATTAATTGATGAGCCGGGCTCTCAGCACATTAGAAAGCAGCTGAGCTTATTAAATAAAGC
TTCCATTTCAACGATCCATTCATTTTGTTTACAAGTTATTAGAGGATACTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATTGCCAATCAAACAGAAAATGAACTATTAAAAGAAGAAGTGCTAGATGACATATTAGAAGAAGAGTATGGAATTGAA
GATAATACGATATTCTTTGAACTTGTTGATCGTTATACGAGTGACCGTAGTGACGATGATTTACAACGTATGATTTTAGC
ACTTCATACAGAATCAAGAGCACATCCAAATCCGGAAAAATGGCTTGATAAATTAGTAGAAGCATATGATGTGGAAGGAA
AGACAATTGAAGATTTAGTGTACGCTTCTTACTTATTAGAAGATGTGAAATTTCAACTTGAAACAGCGGAACAGCATATT
CGTAAAGCGACAGAGCTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCGTTGAAACCCTGCAAGCAGATTTAGTTTTACT
TGGAACGTTATCATCAGCTGCTCGTGAATCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGTATTAAGAAAAGTGATTACAATGAGGATGTTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCGAAAGATGAAGTG
AAGAAATTACAAGAAGAGCTATTTAGCCGTAAGCCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAGCTTGTTCAGCTCGTAAAAGTATTTACAGAACGTTTCCAAACGATGAAGCGAGATAAAGGAATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTGCAAATTTTAAGTGAACAAAGTGAAAATGGTGAAATGAAGCCATCAGCAGTAGCGCTTCAA
TATCGCAATAAATTTGCTGAAGTACTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCGATTATTAAATTCGT
AACGAAAGATTCTGAGAGTGAAGGAAACTTGTTCATGGTTGGTGACGTAAAACAGTCAATTTATCGTTTCCGACTAGCAG
AACCAGGATTATTCTTAGGGAAGTATAAACGTTTCACGCAAGAAGGATTAGGCGGCGGAATGAAGATTGACCTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCAGGTACGAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTCGGGGAAAT
CGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTACCTATCCAGAAGGTGAAGATGTAGCGGCTGAACTACTATGCA
TTCAGCAAACGGAAGAAGAGGTAATAGACGGTGAAGAAGGCGCGGAAGTAGAAAAGGCACAGCTTGAAGCTCGTCTTATG
GCGCAGCGCATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGGCCTGTACAATA
CCGCGACTTCGTTATTTTGCTCCGCTCCATGCCGTGGGCACCACAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCAG
TATACGCTGATCTTGCGACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCCGTACTTCGTTCCCCAATCGTTGGATTAAATGATGAAGAACTTGCAACGCTTCG
TGCTCACGGGAAGAAAGGTTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGCGCACCGCTTGAAGAAGAAAAAGAAT
TACATGATAAATTAGAATGGTTTTACAACTTACTGCAAGGATGGCGTGAATTCGCGCGCCAACAGTCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGGTATTACGACTTTGTCGGTGGTTTACCAGCTGGAAAGCAAAGGCAAGCAAACTT
GCGTGTACTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGACTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGGACGGCAAGAGCTTTAGGTGAACAAGAAGATGTTGTTCGCATTATGACAATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTCGTAGCTGGACTAGGTCGTCGCTTTAATACACAAGACTTAATGAA
ACGTTTCTTACTGCATAAAGACTTCGGTTTCGGTTCGCAATTTATTGATCCACGTAAACGAATTAAATATACGACATTAT
CGCAACTTGCGATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTATTATATGTTGCGTTAACACGT
GCAAAAGAGAAGTTAATTTTAATTGGAACGGTTAAGGATGCAAATAAAGAAATGGAAAAATGGCTTGATGCGAGGGAGCA
TAGTGAATGGTTATTACCAGATCATATACGTGCCGGAGCGTCTTGCTACTTAGACTGGATTGCACCTTCATTATATAGAC
ACCGTGATAGTGAAATACTTCTTGAATTGGGACAAGGAAGCATTCCAGATGAAATTTATGGGTATAGTGCAAGCTGGAAA
GTAGAAGTTGTTGACGGAAACACGTTACTTGCGCCAGAACCAGTTCAAGAAGAGAAGCAAGAGTTGTTAGAAGCACTTCG
TGAGAAAAAGGCCGTTCCTCTGCAAAGTGAACGGAAAGAAGAGGTGTACGATAGATTAATGTGGAAGTACGGATATGAGG
AAGCGACATCTCATCGTGCGAAGCAGTCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAATGCC
TTTATTAAAAAATTACGTGCACCAATTAGAACACGTCCTCGTTTTATGGAGAAAAAAGGTTTAACGTACGCAGAGCGTGG
AACCGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACGATTGAAGTTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAGGAATTATTAACATTTGAACAAGCAGAAGAAATAGCGATTGAAAAAGTAATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
ATATCAAGATTGGCAAGGGAAGAGCGGGGAATCCATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GCATTACGTTAATCGACTTCAAAACAGATACGATTGAAGGGAAATTCCCAGGTGGATTCGAACAAGCGAAACCAATTTTA
GAAGAGCGATATAAAGTGCAGCTTTCGTTATATGCAAAAGCGCTCGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGTAATCATGTTGTAAAAATCGAAGAATAG

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1059-1231)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.828	100	0.538