Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   G4D73_RS06955 Genome accession   NZ_CP053947
Coordinates   1471108..1471908 (-) Length   266 a.a.
NCBI ID   WP_005245984.1    Uniprot ID   -
Organism   Acinetobacter sp. WY4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1466108..1476908
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G4D73_RS06935 - 1467153..1467851 (-) 699 WP_044109972.1 YfgM family protein -
  G4D73_RS06940 hisS 1467882..1469177 (-) 1296 WP_213687686.1 histidine--tRNA ligase -
  G4D73_RS06945 ispG 1469181..1470302 (-) 1122 WP_180038809.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  G4D73_RS06950 - 1470326..1471117 (-) 792 WP_213687687.1 helix-turn-helix domain-containing protein -
  G4D73_RS06955 pilF 1471108..1471908 (-) 801 WP_005245984.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  G4D73_RS06960 rlmN 1471923..1473158 (-) 1236 WP_004278358.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  G4D73_RS06965 ndk 1473281..1473712 (-) 432 WP_004647460.1 nucleoside-diphosphate kinase -
  G4D73_RS06970 iscX 1473823..1474023 (-) 201 WP_004278360.1 Fe-S cluster assembly protein IscX -
  G4D73_RS06975 - 1474111..1474662 (-) 552 WP_004278361.1 phosphatase PAP2 family protein -
  G4D73_RS06980 - 1474683..1475963 (-) 1281 WP_184412636.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30119.24 Da        Isoelectric Point: 9.2878

>NTDB_id=449294 G4D73_RS06955 WP_005245984.1 1471108..1471908(-) (pilF) [Acinetobacter sp. WY4]
MRNLTSKFALISTVCVSLVLVACQTPDLGQKDPEKATKVRTQLAVEYLRTGDLDAAKRALDQALESSPRDSQANMMMGVL
LQQEGSKLNLEKADHYFKRAISADPKNAQARNNYGTYLYQLERYNDAIEQFQIAGATLGYDQRFRALENMGRIYLKLGDA
TNAEKSFKQALQANRDSYISMLELAEIFYLNQQFPAATQMYQQFVRGVGQKNQGARALWVGIRTARAGGDQLGMQVLVNQ
LRALFPESQEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=449294 G4D73_RS06955 WP_005245984.1 1471108..1471908(-) (pilF) [Acinetobacter sp. WY4]
TTGAGAAATCTAACATCAAAGTTTGCTTTGATTTCAACAGTATGTGTGTCTTTGGTTTTAGTCGCGTGTCAGACGCCAGA
TCTGGGTCAGAAAGACCCTGAAAAGGCCACCAAAGTCCGTACCCAATTGGCGGTAGAATATCTGAGAACAGGTGATCTGG
ATGCAGCTAAACGTGCGCTGGATCAGGCACTTGAAAGCAGTCCGCGTGATTCTCAAGCAAATATGATGATGGGCGTGCTA
CTGCAACAGGAAGGTAGTAAGCTTAATCTCGAAAAAGCAGATCATTATTTCAAGCGTGCGATTTCGGCAGATCCGAAAAA
TGCGCAGGCGCGAAATAATTACGGCACTTATTTATATCAGCTCGAACGCTATAATGATGCGATTGAGCAGTTTCAAATTG
CTGGTGCCACGTTGGGTTATGATCAACGTTTCCGCGCACTGGAAAATATGGGGCGGATTTACCTGAAACTGGGTGATGCA
ACAAATGCTGAAAAATCATTCAAACAGGCGCTGCAAGCCAATCGCGATTCTTATATTTCAATGTTAGAGTTGGCAGAAAT
ATTTTATTTGAACCAGCAGTTCCCGGCGGCGACACAAATGTATCAACAGTTTGTGCGTGGTGTGGGACAGAAGAATCAGG
GTGCTCGTGCGCTCTGGGTCGGCATTCGCACTGCGCGTGCCGGGGGTGATCAGTTGGGTATGCAGGTATTGGTCAACCAG
TTACGTGCACTCTTTCCTGAAAGCCAGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(97-129)

Tetratricopeptide repeat (TPR_16)

(42-81)

Tetratricopeptide repeat (TPR_16)

(150-201)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

78.077

97.744

0.763
  pilF Acinetobacter baylyi ADP1

69.173

100

0.692