Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   RGE_RS22465 Genome accession   NC_017075
Coordinates   4930844..4932349 (+) Length   501 a.a.
NCBI ID   WP_014430786.1    Uniprot ID   I0HY51
Organism   Rubrivivax gelatinosus IL144     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4925844..4937349
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RGE_RS22440 (RGE_46000) argS 4926281..4927981 (+) 1701 WP_014430781.1 arginine--tRNA ligase -
  RGE_RS22445 (RGE_46010) - 4927978..4928634 (+) 657 WP_014430782.1 SPOR domain-containing protein -
  RGE_RS22450 (RGE_46020) - 4928699..4929346 (+) 648 WP_014430783.1 thiol:disulfide interchange protein DsbA/DsbL -
  RGE_RS22455 (RGE_46030) lptA 4929458..4930078 (+) 621 WP_052311034.1 lipopolysaccharide transport periplasmic protein LptA -
  RGE_RS22460 (RGE_46040) lptB 4930078..4930836 (+) 759 WP_014430785.1 LPS export ABC transporter ATP-binding protein -
  RGE_RS22465 (RGE_46050) rpoN/rpoN1 4930844..4932349 (+) 1506 WP_014430786.1 RNA polymerase factor sigma-54 Machinery gene
  RGE_RS22470 (RGE_46060) - 4932402..4933640 (+) 1239 WP_014430787.1 class I SAM-dependent RNA methyltransferase -
  RGE_RS22475 (RGE_46070) - 4933637..4934275 (+) 639 WP_014430788.1 DsbA family oxidoreductase -
  RGE_RS22480 (RGE_46080) - 4934276..4935778 (-) 1503 WP_014430789.1 amidase -
  RGE_RS22485 (RGE_46090) - 4935822..4936550 (-) 729 WP_014430790.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  RGE_RS22490 (RGE_46100) - 4936598..4937074 (-) 477 WP_014430791.1 hypothetical protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 55179.29 Da        Isoelectric Point: 4.5915

>NTDB_id=44743 RGE_RS22465 WP_014430786.1 4930844..4932349(+) (rpoN/rpoN1) [Rubrivivax gelatinosus IL144]
MKPSLQVRLSQHLALTPQLQQSIRLLQLSTLELHQEVEQMLEQNPFLEMDEESPTPFDAPAERAAADRVAERDDGGDGAA
EPAPAEVDAAEFGTTERDDWENGTERDDFDGIRETPSSAAPEGDDELDPQERRAGVGTLQDHLRAQVAGMRLSPEDFAAL
NVLIESLDDDGYLADPLEDIAARLADMLDLDDDEQRESLLDRLQCALRWLQSLEPTGVGARSLSECLVLQLRTLSRCEAR
EVAIRICEHSLELLARRDVRKLTAATGAGEDLLRQAQALIVACEPKPGRPFAPAESNIIVPDVIVQKAGRGFKVVLNPDV
MPKLRINDLYAQAIRGQRGSGGGLSSRLQEARWFMKNIQQRFDTILRVSKAIVERQKGFFTHGAIAMKPLVLREIADELG
LHESTISRVTTAKYMATPQGTFELKYFFGSSLNTEAGGNASSTAVRALIQQLVSAEDSAKPLSDSQLSQMLEEQGIQVAR
RTVAKYREALKIAPASLRRAI

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=44743 RGE_RS22465 WP_014430786.1 4930844..4932349(+) (rpoN/rpoN1) [Rubrivivax gelatinosus IL144]
ATGAAGCCGTCCCTGCAGGTTCGCCTGTCCCAGCACCTGGCGTTGACGCCGCAGCTCCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACGCTGGAGCTGCACCAGGAAGTCGAGCAGATGCTCGAGCAGAACCCGTTCCTGGAGATGGACGAGGAGTCGC
CGACGCCCTTCGACGCGCCGGCCGAACGGGCCGCGGCCGACCGCGTCGCCGAGCGCGACGACGGCGGCGACGGTGCCGCC
GAGCCGGCGCCGGCCGAGGTCGACGCCGCCGAGTTCGGCACCACCGAACGCGACGACTGGGAGAACGGCACCGAACGCGA
CGACTTCGACGGCATCCGCGAGACGCCGAGTTCGGCGGCGCCCGAAGGCGACGACGAGCTCGACCCGCAGGAGCGCCGCG
CCGGCGTCGGCACGCTGCAGGACCATCTGCGCGCCCAGGTCGCCGGCATGCGTTTGTCGCCCGAGGACTTCGCGGCGCTG
AACGTGCTGATCGAGTCGCTGGACGACGACGGCTACCTCGCCGACCCGCTGGAAGACATCGCCGCGCGCCTGGCCGACAT
GCTCGACCTGGACGACGACGAGCAGCGCGAGAGCCTGCTCGATCGCCTGCAGTGCGCGCTGCGCTGGCTGCAGAGCCTGG
AGCCGACCGGCGTCGGCGCGCGCTCGTTGTCCGAGTGCCTGGTGCTGCAGCTGCGCACGCTGTCGCGCTGCGAGGCGCGC
GAGGTCGCGATCCGCATCTGCGAGCACTCGCTGGAGCTGCTGGCGCGGCGCGACGTGCGCAAGCTGACCGCGGCCACCGG
CGCCGGCGAGGATCTGCTGCGCCAGGCGCAGGCGCTGATCGTCGCCTGCGAGCCCAAGCCCGGGCGGCCGTTCGCGCCGG
CCGAGTCGAACATCATCGTGCCCGACGTCATCGTCCAGAAGGCCGGGCGCGGCTTCAAGGTCGTGCTCAACCCGGACGTG
ATGCCCAAGCTGCGCATCAACGACCTCTACGCCCAGGCGATCCGCGGCCAGCGCGGCAGCGGCGGCGGGTTGTCGTCGCG
GCTGCAGGAGGCGCGCTGGTTCATGAAGAACATCCAGCAGCGCTTCGACACCATCCTGCGCGTGTCCAAGGCCATCGTCG
AGCGCCAGAAGGGCTTCTTCACCCACGGCGCGATCGCGATGAAGCCGCTGGTGCTGCGCGAGATCGCCGACGAGCTGGGC
CTGCACGAGAGCACGATCTCGCGCGTGACGACGGCCAAGTACATGGCCACGCCGCAAGGCACCTTCGAGCTGAAGTACTT
CTTCGGCAGCTCGCTGAACACCGAGGCCGGCGGCAACGCGTCGAGCACCGCGGTGCGCGCGCTGATCCAGCAGCTGGTCT
CGGCCGAGGACAGCGCCAAGCCGCTGTCGGACAGCCAGCTCTCGCAAATGCTCGAGGAGCAGGGCATCCAGGTCGCACGC
CGCACCGTCGCCAAGTACCGCGAGGCGCTGAAGATCGCGCCGGCCTCGCTGCGCCGCGCGATCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-49)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(133-330)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I0HY51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

53.891

100

0.553

Multiple sequence alignment