Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   I5U72_RS29310 Genome accession   NZ_CP065417
Coordinates   6315910..6316506 (-) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa isolate P23     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6310910..6321506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5U72_RS29290 (I5U72_29290) aroK 6312041..6312559 (-) 519 WP_003095833.1 shikimate kinase AroK -
  I5U72_RS29295 (I5U72_29295) pilQ 6312571..6314715 (-) 2145 WP_003123619.1 type 4a pilus secretin PilQ Machinery gene
  I5U72_RS29300 (I5U72_29300) comL/pilP 6314769..6315293 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  I5U72_RS29305 (I5U72_29305) pilO 6315290..6315913 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  I5U72_RS29310 (I5U72_29310) pilN 6315910..6316506 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  I5U72_RS29315 (I5U72_29315) pilM 6316506..6317570 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  I5U72_RS29320 (I5U72_29320) - 6317755..6320226 (+) 2472 WP_031756300.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=446939 I5U72_RS29310 WP_003095839.1 6315910..6316506(-) (pilN) [Pseudomonas aeruginosa isolate P23]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=446939 I5U72_RS29310 WP_003095839.1 6315910..6316506(-) (pilN) [Pseudomonas aeruginosa isolate P23]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCTGCACTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGACTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGGCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATCGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGCTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369