Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HPQ68_RS05355 Genome accession   NZ_CP053748
Coordinates   1165983..1167458 (-) Length   491 a.a.
NCBI ID   WP_255756775.1    Uniprot ID   -
Organism   Massilia sp. erpn     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1160983..1172458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPQ68_RS05325 (HPQ68_05310) - 1161411..1161893 (+) 483 WP_050409689.1 PTS sugar transporter subunit IIA -
  HPQ68_RS05330 (HPQ68_05315) hprK 1161929..1162864 (+) 936 WP_050409690.1 HPr(Ser) kinase/phosphatase -
  HPQ68_RS05335 (HPQ68_05320) rapZ 1162998..1163858 (+) 861 WP_255756772.1 RNase adapter RapZ -
  HPQ68_RS05340 (HPQ68_05325) - 1163865..1164239 (-) 375 WP_176347847.1 VOC family protein -
  HPQ68_RS05345 (HPQ68_05330) - 1164338..1165426 (-) 1089 WP_255756773.1 enoyl-CoA hydratase/isomerase family protein -
  HPQ68_RS05350 (HPQ68_05335) hpf 1165550..1165909 (-) 360 WP_255756774.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  HPQ68_RS05355 (HPQ68_05340) rpoN/rpoN1 1165983..1167458 (-) 1476 WP_255756775.1 RNA polymerase factor sigma-54 Machinery gene
  HPQ68_RS05360 (HPQ68_05345) lptB 1167463..1168218 (-) 756 WP_255756776.1 LPS export ABC transporter ATP-binding protein -
  HPQ68_RS05365 (HPQ68_05350) lptA 1168221..1168808 (-) 588 WP_050409697.1 lipopolysaccharide transport periplasmic protein LptA -
  HPQ68_RS05370 (HPQ68_05355) lptC 1168834..1169427 (-) 594 WP_255756777.1 LPS export ABC transporter periplasmic protein LptC -
  HPQ68_RS05375 (HPQ68_05360) - 1169442..1169981 (-) 540 WP_255756778.1 HAD family hydrolase -
  HPQ68_RS05380 (HPQ68_05365) - 1169981..1170979 (-) 999 WP_176347855.1 SIS domain-containing protein -

Sequence

Protein


Download         Length: 491 a.a.        Molecular weight: 54318.90 Da        Isoelectric Point: 5.0358

>NTDB_id=446883 HPQ68_RS05355 WP_255756775.1 1165983..1167458(-) (rpoN/rpoN1) [Massilia sp. erpn]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELHQELEQLLTDNPLLERLDDPLDRSVRLLADGAISPTSTPGDAPPDNA
APADAPAPAAEAEPAYEGGEGESVAEADGDWGDTGRGKAPEDDDARPQLEAHHCSLREHLLEQMRVTLQEPRDRALMELI
ADALDDNGYLDESLEEIHARLPLELDVELEELQTSLTLLQSFDPAGVGARSAAECLALQIRRLPGVPLVTRRMALCIVEK
HLAWFAQREFTKLKKALDCDDEDLREAQAVIRQCNPHPGAAFAADVSDYVVPDVVVKKGRNGWIVMLNNDVMPRLRVNSL
YASLLRQGKGESQMSAQLQEAKWLIKNMRQRFDTILRVAQAIVERQKNFFSHGAVAMRPLVLREIADTLGLHESTISRVT
TQKYMLTPHGMFELKYFFGSHVATEAGGEASSTAIRALIVQLTGAEDPKNPLSDSKIADMLGEQGMVIARRTVAKYREAL
KIPPVSLRKSL

Nucleotide


Download         Length: 1476 bp        

>NTDB_id=446883 HPQ68_RS05355 WP_255756775.1 1165983..1167458(-) (rpoN/rpoN1) [Massilia sp. erpn]
ATGAAACAGTCTTTGCAACTGCGCACCTCGCAACACCTCGCGTTGACGCCGCAGTTGCAGCAGTCCATCCGTCTGCTGCA
ACTGTCCACGCTGGAACTGCATCAGGAACTGGAACAGCTGCTGACTGATAATCCCCTGCTGGAGCGTCTCGACGATCCGC
TCGACCGCTCCGTGCGCCTGCTGGCCGATGGCGCCATCAGTCCGACCTCCACACCCGGCGATGCGCCGCCGGACAATGCC
GCGCCGGCCGATGCGCCCGCGCCGGCCGCCGAGGCCGAACCCGCCTATGAAGGCGGCGAAGGCGAGAGCGTGGCCGAAGC
CGATGGCGACTGGGGCGACACGGGCCGCGGCAAGGCGCCCGAAGACGACGACGCCCGTCCCCAGCTGGAAGCCCACCACT
GCAGCCTGCGCGAGCATTTGCTGGAGCAGATGCGCGTCACCCTGCAGGAGCCGCGCGACCGCGCCTTGATGGAACTGATC
GCCGATGCGCTGGACGACAACGGTTATCTGGACGAGAGCCTGGAGGAAATCCACGCGCGCCTGCCGCTGGAACTCGATGT
GGAGCTGGAAGAGCTGCAAACTTCGCTCACCCTGCTGCAAAGCTTCGATCCGGCCGGCGTCGGCGCGCGCAGCGCCGCCG
AATGCCTGGCGCTGCAAATCCGCCGCCTGCCCGGCGTGCCTTTGGTGACGCGGCGCATGGCTTTATGCATCGTCGAAAAG
CACCTGGCCTGGTTTGCCCAGCGCGAATTCACCAAGCTGAAAAAAGCCCTCGACTGCGACGACGAAGACCTGCGCGAAGC
GCAGGCCGTGATCCGCCAATGCAATCCGCATCCCGGCGCCGCCTTCGCTGCCGACGTGTCGGACTACGTGGTGCCGGACG
TGGTGGTGAAGAAGGGCCGCAATGGCTGGATCGTGATGCTGAATAACGATGTGATGCCGCGCCTGCGCGTGAACTCCCTG
TACGCCAGCCTGCTGCGCCAGGGGAAAGGCGAATCGCAGATGAGCGCCCAGCTGCAGGAGGCGAAGTGGCTGATCAAGAA
TATGCGCCAGCGCTTTGACACAATTTTGCGCGTGGCGCAGGCCATCGTGGAGCGTCAAAAGAATTTCTTCTCGCACGGCG
CCGTGGCCATGAGACCCCTTGTGTTGCGTGAAATTGCTGATACACTGGGACTACACGAGAGCACTATTTCTCGCGTAACA
ACTCAGAAATATATGCTCACCCCCCATGGCATGTTTGAGTTGAAATATTTTTTTGGCAGCCATGTCGCGACGGAAGCGGG
GGGCGAAGCTTCTTCCACGGCGATACGGGCGCTGATTGTGCAATTGACAGGAGCAGAAGACCCGAAAAATCCTTTATCCG
ACAGTAAGATTGCGGACATGCTGGGAGAACAAGGCATGGTAATTGCGCGACGCACTGTTGCCAAATATCGAGAAGCATTG
AAGATCCCGCCAGTCAGCCTCCGCAAGTCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(130-321)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(333-489)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

60.938

100

0.635