Detailed information    

insolico Bioinformatically predicted

Overview

Name   comF   Type   Machinery gene
Locus tag   I5U70_RS26845 Genome accession   NZ_CP065412
Coordinates   5764681..5765106 (+) Length   141 a.a.
NCBI ID   WP_003094721.1    Uniprot ID   G3XD43
Organism   Pseudomonas aeruginosa isolate P33     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5759681..5770106
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5U70_RS26830 (I5U70_26840) pilX 5760245..5760832 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -
  I5U70_RS26835 (I5U70_26845) pilY1 5760844..5764335 (+) 3492 WP_003102607.1 type 4a pilus biogenesis protein PilY1 -
  I5U70_RS26840 (I5U70_26850) pilY2 5764337..5764684 (+) 348 WP_003102609.1 type 4a fimbrial biogenesis protein PilY2 -
  I5U70_RS26845 (I5U70_26855) comF 5764681..5765106 (+) 426 WP_003094721.1 type 4a pilus minor pilin PilE Machinery gene
  I5U70_RS26850 (I5U70_26860) ispH 5765153..5766097 (-) 945 WP_003094724.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  I5U70_RS26855 (I5U70_26865) fkpB 5766183..5766623 (-) 441 WP_003102613.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  I5U70_RS26860 (I5U70_26870) lspA 5766616..5767125 (-) 510 WP_003110420.1 signal peptidase II -
  I5U70_RS26865 (I5U70_26875) ileS 5767118..5769949 (-) 2832 WP_023103515.1 isoleucine--tRNA ligase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 15279.30 Da        Isoelectric Point: 10.0198

>NTDB_id=446830 I5U70_RS26845 WP_003094721.1 5764681..5765106(+) (comF) [Pseudomonas aeruginosa isolate P33]
MRTRQKGFTLLEMVVVVAVIGILLGIAIPSYQNYVIRSNRTEGQALLSDAAARQERYYSQNPGVGYTKDVAKLGMSSANS
PNNLYNLTIATPTSTTYTLTATPINSQTRDKTCGKLTLNQLGERGAAGKTGNNSTVNDCWR

Nucleotide


Download         Length: 426 bp        

>NTDB_id=446830 I5U70_RS26845 WP_003094721.1 5764681..5765106(+) (comF) [Pseudomonas aeruginosa isolate P33]
ATGAGGACAAGACAGAAGGGCTTCACGTTGCTGGAAATGGTGGTGGTAGTGGCGGTGATCGGCATCCTCCTCGGCATCGC
CATTCCCAGTTACCAGAACTACGTGATCCGCTCCAACCGCACCGAGGGCCAGGCCCTGCTCTCGGACGCGGCCGCGCGCC
AGGAACGCTACTACTCGCAGAACCCCGGGGTCGGCTACACCAAGGACGTGGCCAAGCTGGGCATGAGTTCGGCCAACTCG
CCGAACAACCTGTACAATCTCACCATAGCGACGCCCACCAGCACCACCTATACCCTGACCGCCACGCCGATCAACTCGCA
GACCCGCGACAAGACCTGCGGCAAGCTGACCCTCAATCAGCTCGGCGAACGCGGCGCAGCCGGCAAGACCGGCAACAACA
GCACCGTCAACGACTGCTGGCGCTGA

Domains

Predicted by InterProScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(29-117)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 4NOA

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comF Acinetobacter baylyi ADP1

42.188

90.78

0.383