Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilH   Type   Machinery gene
Locus tag   I5U70_RS02260 Genome accession   NZ_CP065412
Coordinates   481858..482223 (+) Length   121 a.a.
NCBI ID   WP_003084587.1    Uniprot ID   A0A0H2ZKW2
Organism   Pseudomonas aeruginosa isolate P33     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 476858..487223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5U70_RS02230 (I5U70_02230) pyrR 477480..477992 (-) 513 WP_003084574.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  I5U70_RS02235 (I5U70_02235) ruvX 478104..478538 (-) 435 WP_003084575.1 Holliday junction resolvase RuvX -
  I5U70_RS02240 (I5U70_02240) - 478538..479107 (-) 570 WP_003084577.1 YqgE/AlgH family protein -
  I5U70_RS02245 (I5U70_02245) - 479156..480049 (-) 894 WP_003162015.1 energy transducer TonB -
  I5U70_RS02250 (I5U70_02250) gshB 480196..481149 (-) 954 WP_003100943.1 glutathione synthase -
  I5U70_RS02255 (I5U70_02255) pilG 481404..481811 (+) 408 WP_003084583.1 twitching motility response regulator PilG Regulator
  I5U70_RS02260 (I5U70_02260) pilH 481858..482223 (+) 366 WP_003084587.1 twitching motility response regulator PilH Machinery gene
  I5U70_RS02265 (I5U70_02265) - 482274..482810 (+) 537 WP_003084590.1 chemotaxis protein CheW -
  I5U70_RS02270 (I5U70_02270) pilJ 482895..484943 (+) 2049 WP_003100940.1 chemotaxis chemoreceptor PilJ -
  I5U70_RS02275 (I5U70_02275) pilK 485004..485879 (+) 876 WP_031757002.1 type 4 fimbrial methyltransferase PilK -

Sequence

Protein


Download         Length: 121 a.a.        Molecular weight: 13273.30 Da        Isoelectric Point: 5.1961

>NTDB_id=446762 I5U70_RS02260 WP_003084587.1 481858..482223(+) (pilH) [Pseudomonas aeruginosa isolate P33]
MARILIVDDSPTEMYKLTAMLEKHGHQVLKAENGGDGVALARQEKPDVVLMDIVMPGLNGFQATRQLTKDAETSAIPVII
VTTKDQETDKVWGKRQGARDYLTKPVDEETLLKTINAVLAG

Nucleotide


Download         Length: 366 bp        

>NTDB_id=446762 I5U70_RS02260 WP_003084587.1 481858..482223(+) (pilH) [Pseudomonas aeruginosa isolate P33]
ATGGCTCGTATTTTGATTGTTGATGACTCTCCGACCGAGATGTACAAGCTGACCGCCATGCTGGAAAAGCATGGTCACCA
GGTACTCAAGGCCGAGAACGGCGGCGACGGCGTCGCCCTGGCCCGCCAGGAAAAGCCCGACGTGGTCCTGATGGACATCG
TCATGCCCGGCCTCAACGGCTTCCAGGCGACCCGTCAATTGACCAAGGACGCCGAGACCAGCGCCATCCCGGTGATCATC
GTCACCACCAAGGACCAGGAGACCGACAAGGTCTGGGGCAAGCGCCAGGGCGCTCGCGACTACCTGACCAAGCCGGTGGA
CGAAGAGACCCTGCTGAAAACCATCAATGCGGTGCTGGCGGGCTGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(4-116)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZKW2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilH Synechocystis sp. PCC 6803

36.975

98.347

0.364