Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   HPK29_RS12425 Genome accession   NZ_CP053704
Coordinates   2569750..2571120 (-) Length   456 a.a.
NCBI ID   WP_002343125.1    Uniprot ID   A0A828ZZY3
Organism   Enterococcus faecium strain FS 86     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2564750..2576120
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPK29_RS12410 (HPK29_12440) epsC 2566070..2566606 (-) 537 WP_002293094.1 serine O-acetyltransferase EpsC -
  HPK29_RS12415 (HPK29_12445) gltX 2566929..2568389 (-) 1461 WP_002293095.1 glutamate--tRNA ligase -
  HPK29_RS12420 (HPK29_12450) - 2568559..2569716 (-) 1158 WP_002287448.1 PIN/TRAM domain-containing protein -
  HPK29_RS12425 (HPK29_12455) radA 2569750..2571120 (-) 1371 WP_002343125.1 DNA repair protein RadA Machinery gene
  HPK29_RS12430 (HPK29_12460) - 2571183..2571710 (-) 528 WP_002293097.1 dUTP diphosphatase -
  HPK29_RS12435 (HPK29_12465) proB 2571940..2572746 (+) 807 WP_002287451.1 glutamate 5-kinase -
  HPK29_RS12440 (HPK29_12470) - 2572743..2573990 (+) 1248 WP_002329828.1 glutamate-5-semialdehyde dehydrogenase -
  HPK29_RS12445 (HPK29_12475) - 2574066..2574410 (+) 345 WP_002293100.1 hypothetical protein -
  HPK29_RS12450 (HPK29_12480) - 2574665..2574985 (-) 321 WP_002293149.1 hypothetical protein -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49965.69 Da        Isoelectric Point: 7.7416

>NTDB_id=446457 HPK29_RS12425 WP_002343125.1 2569750..2571120(-) (radA) [Enterococcus faecium strain FS 86]
MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTDRRTRTSLTGEKAKPTKIADVVPKKEPRVKMKLEELN
RVLGGGVVPGSMVLIGGDPGIGKSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEVANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECFIGEIGLTG
EIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAETLKRVFR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=446457 HPK29_RS12425 WP_002343125.1 2569750..2571120(-) (radA) [Enterococcus faecium strain FS 86]
ATGGCAAAAAAATCAAAAGTACAATTTGTTTGCCAAAATTGCGGGTATGTGTCGCCTAAGTTCTTGGGACGCTGCCCAAA
TTGCGGAAAATGGAATACGATGGTCGAAGAAATCGAACAAGATACAACAGACCGCAGAACGCGAACAAGTTTGACAGGAG
AAAAAGCGAAACCAACAAAAATAGCAGACGTCGTCCCTAAAAAAGAACCTCGGGTCAAAATGAAATTAGAAGAACTGAAC
CGTGTGCTAGGTGGAGGTGTGGTACCGGGATCCATGGTCTTGATCGGTGGTGATCCAGGTATTGGTAAATCCACTTTGCT
ATTACAAGTTTCTCAGCAATTAGCAGCCATAGGCGGGAAAGTCTTGTATGTATCCGGCGAAGAAAGCGCAGAACAGATAA
AATTACGCGCGGAGCGCCTCGGAAGTATCAACACAGAATTTTATCTATACGCTGAAACAGATATGAATGAAATCAGTCGT
GCCATCGAACATATCTCCCCTGATTATGTTATTATTGATTCCATCCAAACGATGACGCAGCCAGACATCACAAGTGTGGC
AGGAAGCGTCAGCCAAGTAAGAGAAACCACTGCAGAATTACTCAAAATCGCTAAAACAAATGGGATTGCCATCTTTATCG
TCGGTCATGTTACGAAAGAAGGTTCGATCGCCGGTCCAAGAATGTTAGAGCATATGGTGGATACAGTGTTATATTTTGAA
GGTGAACAACATCATAGTTTCCGAATTTTACGAGCGGTAAAAAACCGATTTGGCTCAACGAACGAGATTGGGATTTTTGA
AATGCATGAGCATGGATTGGAAGAAGTCGCTAACCCTTCACAGATTTTCTTAGAGGAACGCCTAGACGGTGCGACTGGCT
CTGCTATCGTTGTAGCAATGGAAGGAACACGTCCAATTTTGGTAGAGATCCAAGCATTAGTCACACCTACTATGTTCGGA
AATGCTAAGCGAACAACAACAGGTCTTGATTTCAATCGTGTATCTCTGATCATGGCAGTGCTGGAAAAAAGAGCTGGACT
ACTTCTTCAGAATCAAGATGCTTACTTAAAAGCTGCAGGTGGGGTAAAATTAAATGAACCAGCGATTGATTTAGCTATCG
CTGTCAGTATTGCTTCAAGCTACAAAGAAAAAGGAACACAGCCGACGGAATGTTTCATCGGTGAAATCGGACTGACTGGA
GAAATCAGACGTGTCAGTCATATTGAACAACGGGTCAAAGAAGTCCAAAAACTCGGCTTCACAAAAGTTTACCTGCCTAA
AAACAACTTAGGCAACTGGGAAGCGCCAAAAGGAATCGAGATCGTTGGAGTAGCAACTTTGGCTGAAACGTTGAAGCGTG
TGTTCCGCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(72-221)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A828ZZY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

74.066

99.781

0.739
  radA Streptococcus pneumoniae Rx1

74.066

99.781

0.739
  radA Streptococcus pneumoniae D39

74.066

99.781

0.739
  radA Streptococcus pneumoniae R6

74.066

99.781

0.739
  radA Streptococcus pneumoniae TIGR4

74.066

99.781

0.739
  radA Streptococcus mitis SK321

73.846

99.781

0.737
  radA Bacillus subtilis subsp. subtilis str. 168

67.406

98.904

0.667