Detailed information NTG0446448

Bioinformatically predicted

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: I5M77_RS12395
• Genome accession: NZ_CP065369
• Coordinates: 2599637..2601841 (+)
• Length: 734 a.a.
• NCBI ID: WP_029824531.1
• Uniprot ID: -
• Organism: Vibrio parahaemolyticus strain 20-082A3
• Function: DNA puliing through the inner membrane (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2594637..2606841

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I5M77_RS12355 (I5M77_12355)	rplV	2595306..2595638 (-)	333	WP_005383164.1	50S ribosomal protein L22	-
I5M77_RS12360 (I5M77_12360)	rpsS	2595649..2595927 (-)	279	WP_004394525.1	30S ribosomal protein S19	-
I5M77_RS12365 (I5M77_12365)	rplB	2595949..2596773 (-)	825	WP_005489461.1	50S ribosomal protein L2	-
I5M77_RS12370 (I5M77_12370)	rplW	2596789..2597091 (-)	303	WP_004398471.1	50S ribosomal protein L23	-
I5M77_RS12375 (I5M77_12375)	rplD	2597088..2597690 (-)	603	WP_005379556.1	50S ribosomal protein L4	-
I5M77_RS12380 (I5M77_12380)	rplC	2597708..2598337 (-)	630	WP_005456132.1	50S ribosomal protein L3	-
I5M77_RS12385 (I5M77_12385)	rpsJ	2598352..2598663 (-)	312	WP_004410492.1	30S ribosomal protein S10	-
I5M77_RS12390 (I5M77_12390)	rpmE	2599121..2599342 (-)	222	WP_005457203.1	50S ribosomal protein L31	-
I5M77_RS12395 (I5M77_12395)	priA	2599637..2601841 (+)	2205	WP_029824531.1	primosomal protein N'	Machinery gene
I5M77_RS12400 (I5M77_12400)	cytR	2602174..2603181 (+)	1008	WP_005481416.1	DNA-binding transcriptional regulator CytR	Regulator
I5M77_RS12405 (I5M77_12405)	ftsN	2603358..2603903 (+)	546	WP_005481405.1	cell division protein FtsN	-
I5M77_RS12410 (I5M77_12410)	hslV	2604068..2604619 (+)	552	WP_005489705.1	ATP-dependent protease subunit HslV	-
I5M77_RS12415 (I5M77_12415)	hslU	2604643..2605974 (+)	1332	WP_005489452.1	HslU--HslV peptidase ATPase subunit	-
I5M77_RS12420 (I5M77_12420)	-	2606398..2606622 (-)	225	WP_005489715.1	hypothetical protein	-

Sequence

Protein

• Length: 734 a.a.
• Molecular weight: 82241.85 Da
• Isoelectric Point: 8.6772

>NTDB_id=446448 I5M77_RS12395 WP_029824531.1 2599637..2601841(+) (priA) [Vibrio parahaemolyticus strain 20-082A3]
MRPSIARVALPVPLDKQFDYAIPGHLFPIIGGRVSVPFGRQTLVGIVTAMVNHSDFPKDQLKPIKAVLDSQPIWPEKLYS
LLTWCSQFYQYPLGDTLHNAMPAALRKGKPADFATLQEWQITESGKDKLMQGLDRRAVKQQKVLQMLVNGALPHQEFVDQ
EIASTVLKSLEEKGWIERIEKKPVITQWGQHVECDVEKPKLNHEQALAIASVNSQTGFACYLLEGVTGSGKTEVYLNLIK
PVLEKGKQALVLVPEIGLTPQTINRFKRRFNVPVDVIHSGLNETERLNAWLSARDKAAGIIIGTRSALLAPFADLGIIIV
DEEHDTSYKQQDSLRYHARDVAVMRAHKEQIPIVLGSATPALETLHNALSGKYQHLTLTQRAGSAVPTTNKVLDVKGQYL
ESGLSAPLIAEMRKHLKAGNQVMLFLNRRGFSPALMCHECGWIAECKRCDAYYTFHQYSNEIRCHHCGSQHPVIHQCQGC
GSTQLVTVGVGTEQLEQQLAQLFPEYKAIRIDRDSTRRKGSLEDALDSIRKGEYQILIGTQMLAKGHHFPNVTLVALLDV
DGSLYSSDFRASERLAQLFIQVAGRAGRASKPGEVVLQTHHPEHSLLQALLEKDYRHFAMTALEERKLAQLPPYSFLTLF
KAEANQSEIVEDFLRQVRFTLESHPLFDGTCMVLGPTPSPLAKRAGKYRWQLLLQTQHRSLMQKLLTSAKPAIELLPNAK
KVRWNLDIEPQDLS

Nucleotide

• Length: 2205 bp

>NTDB_id=446448 I5M77_RS12395 WP_029824531.1 2599637..2601841(+) (priA) [Vibrio parahaemolyticus strain 20-082A3]
ATGCGTCCATCCATTGCTAGAGTGGCACTGCCCGTACCTCTCGACAAACAATTTGACTATGCCATCCCAGGACACCTGTT
TCCTATCATTGGCGGGCGCGTATCCGTACCTTTTGGACGTCAAACGTTAGTTGGCATCGTCACCGCGATGGTTAATCACT
CGGACTTTCCCAAAGATCAACTCAAGCCAATCAAAGCGGTTCTTGATTCTCAGCCGATATGGCCTGAAAAGCTTTATTCG
TTGCTGACTTGGTGCAGCCAGTTCTACCAATACCCACTTGGAGATACGCTACACAATGCGATGCCAGCAGCTCTGCGTAA
AGGAAAGCCTGCAGATTTTGCGACGCTACAAGAGTGGCAAATTACCGAATCCGGTAAAGACAAGCTGATGCAAGGGCTTG
ATCGTCGAGCGGTCAAACAACAGAAGGTCTTGCAAATGTTGGTTAACGGCGCTTTGCCTCACCAGGAATTTGTCGACCAA
GAGATAGCCTCAACAGTACTAAAGTCTTTAGAAGAGAAAGGCTGGATTGAACGTATCGAGAAGAAGCCTGTAATCACCCA
GTGGGGACAGCACGTTGAATGCGACGTCGAGAAGCCAAAGCTCAACCACGAACAAGCGCTGGCTATTGCGAGTGTAAATA
GCCAAACAGGTTTTGCTTGCTATTTATTAGAGGGCGTCACGGGCTCAGGTAAGACAGAAGTGTACCTTAACCTGATCAAG
CCGGTCTTAGAGAAAGGTAAACAAGCTTTGGTTTTAGTACCAGAGATTGGCTTAACACCGCAAACCATCAACCGCTTTAA
ACGCCGTTTTAATGTGCCAGTTGACGTTATCCACTCCGGATTAAACGAGACGGAGCGTCTGAATGCGTGGCTTTCTGCAC
GCGACAAAGCGGCCGGAATCATTATCGGCACACGCTCTGCCCTACTCGCGCCTTTTGCTGATCTCGGGATTATCATTGTG
GACGAGGAACATGACACCTCTTACAAACAGCAAGATAGCTTGCGTTATCATGCACGCGATGTTGCTGTAATGCGCGCTCA
TAAAGAACAAATTCCGATTGTTCTGGGCTCAGCCACACCTGCACTGGAGACATTGCACAATGCCTTGTCAGGCAAGTATC
AGCATTTAACCCTGACCCAACGCGCAGGCTCTGCGGTTCCTACTACTAATAAAGTTCTCGACGTCAAAGGTCAGTATTTA
GAAAGTGGTTTATCTGCCCCATTGATTGCGGAAATGCGTAAACACCTTAAAGCAGGCAATCAGGTGATGCTGTTTCTTAA
CCGTCGTGGTTTCTCCCCTGCTCTCATGTGTCATGAATGTGGTTGGATTGCCGAATGCAAACGTTGTGATGCTTATTACA
CCTTCCACCAATACAGCAATGAGATTCGCTGCCACCATTGTGGCTCACAGCACCCAGTGATTCATCAGTGTCAGGGCTGT
GGTTCAACTCAACTGGTTACCGTTGGTGTCGGTACCGAACAGTTAGAACAACAATTGGCACAATTGTTTCCCGAATACAA
AGCTATCCGTATCGACAGGGATAGTACGCGCCGCAAAGGTAGCTTAGAAGACGCGTTGGACTCCATCCGCAAGGGTGAAT
ATCAAATTTTAATTGGCACTCAAATGTTAGCTAAGGGACACCATTTCCCCAATGTAACGCTCGTTGCCTTGCTAGATGTT
GATGGCTCTCTATATAGTAGCGACTTCCGCGCCTCCGAAAGATTAGCGCAGTTATTCATTCAAGTCGCAGGGCGAGCCGG
ACGTGCAAGTAAGCCGGGGGAAGTGGTTTTGCAGACTCACCATCCTGAGCACAGTTTGTTGCAAGCCTTATTGGAAAAGG
ACTACCGACACTTCGCTATGACGGCATTAGAGGAACGTAAACTGGCGCAGTTGCCACCTTACAGTTTCCTGACTCTATTT
AAAGCCGAAGCGAATCAGAGCGAGATCGTAGAAGATTTTCTGCGCCAAGTTCGCTTTACCTTAGAATCGCACCCTTTGTT
TGACGGCACCTGCATGGTGCTTGGTCCAACACCATCGCCTCTCGCCAAACGCGCAGGCAAATACCGTTGGCAGCTGTTAC
TCCAAACCCAGCACCGTTCATTAATGCAAAAGTTATTAACCAGTGCAAAACCTGCTATTGAGTTGTTGCCTAATGCCAAA
AAAGTTCGCTGGAATTTAGACATAGAACCACAAGATCTCAGCTAG

Domains

Predicted by InterProScan.

• 3DNA-binding domain (3BD) (PriA_3primeBD) (7-102)

• PriA DNA helicase Cys-rich region (CRR) domain (Zn_ribbon_PriA) (446-472)

• Primosomal protein N C-terminal domain (PriA_C) (633-730)

• Helicase conserved C-terminal domain (Helicase_C) (500-589)

• DEAD/DEAH box helicase (DEAD) (220-365)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
priA	Acinetobacter baumannii D1279779	43.649	100	0.44
priA	Bacillus subtilis subsp. subtilis str. 168	34.005	100	0.368