Detailed information NTG0446030

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: HPG46_RS25330
• Genome accession: NZ_CP053656
• Coordinates: 4864558..4865907 (-)
• Length: 449 a.a.
• NCBI ID: WP_000879417.1
• Uniprot ID: A0AAE9TF02
• Organism: Bacillus cereus strain CTMA_1571
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4859558..4870907

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HPG46_RS25310 (HPG46_24920)	secA	4859880..4862387 (-)	2508	WP_000579383.1	preprotein translocase subunit SecA	-
HPG46_RS25315 (HPG46_24925)	raiA	4862664..4863206 (-)	543	WP_000671189.1	ribosome-associated translation inhibitor RaiA	-
HPG46_RS25320 (HPG46_24930)	cspC	4863530..4863727 (-)	198	WP_001990088.1	cold shock protein CspC	-
HPG46_RS25325 (HPG46_24935)	comFC	4863854..4864558 (-)	705	WP_003162242.1	comF operon protein ComFC	-
HPG46_RS25330 (HPG46_24940)	comFA	4864558..4865907 (-)	1350	WP_000879417.1	ATP-dependent helicase ComFA	Machinery gene
HPG46_RS25335 (HPG46_24945)	-	4866035..4867463 (-)	1429	Protein_4957	NlpC/P60 family protein	-
HPG46_RS25340 (HPG46_24950)	-	4867611..4867925 (-)	315	WP_000400857.1	helix-turn-helix domain-containing protein	-
HPG46_RS25345 (HPG46_24955)	-	4868099..4868941 (-)	843	WP_000844766.1	DegV family protein	-
HPG46_RS25350 (HPG46_24960)	-	4869181..4869816 (+)	636	WP_000926685.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51233.89 Da
• Isoelectric Point: 9.5047

>NTDB_id=446030 HPG46_RS25330 WP_000879417.1 4864558..4865907(-) (comFA) [Bacillus cereus strain CTMA_1571]
MLAGKQLLLEELPSDLRRELSDLKKEGEVICVQGVIKKASKYICQRCGNIEQRLFASFLCKRCSKVCTYCRKCITMGRVS
ECAVLVRGIHERKGERELHSLQWKGSLSLGQELAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGIEEALQKGERVCIAT
PRTDVVLELVPRLQEVFPSINVAALYGGSVDREKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KEKAARIYLTATPDEKWKRNFRTGKQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLHHKKIPRVLLQWLKMYVNKKYPIFL
FVPHVRYIEEIGLLLKGLDHRIDGVHAEDPMRKEKVEAFRKGDIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVVYFHYGKTESMVRAKRHIQSMNKSAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=446030 HPG46_RS25330 WP_000879417.1 4864558..4865907(-) (comFA) [Bacillus cereus strain CTMA_1571]
ATGCTCGCTGGAAAGCAGTTACTATTAGAAGAACTCCCTTCAGATTTACGGAGAGAATTAAGTGATTTGAAAAAGGAGGG
AGAGGTCATATGTGTACAAGGCGTAATAAAGAAGGCTTCTAAATATATATGTCAGCGCTGCGGAAATATAGAGCAGCGGC
TATTTGCATCATTTTTATGTAAAAGGTGCAGTAAAGTATGTACGTATTGCCGGAAGTGTATAACGATGGGGAGAGTTAGT
GAATGTGCTGTACTTGTTCGCGGGATTCATGAAAGAAAAGGAGAAAGGGAATTACATTCGTTACAGTGGAAAGGGAGTTT
GTCTCTTGGTCAGGAGCTGGCGGCGCAAGGTGTTATAGAAGCTATTAAGCAAAAAGAATCTTTTTTTATTTGGGCTGTGT
GCGGGGCTGGAAAAACAGAAATGTTATTTTACGGTATAGAAGAGGCACTTCAAAAAGGAGAAAGAGTGTGTATCGCAACG
CCAAGGACGGACGTTGTACTGGAATTAGTACCGAGATTACAAGAAGTGTTTCCAAGTATAAATGTAGCTGCTTTATACGG
AGGGAGTGTAGATCGTGAAAAAGATGCAGCGTTAGTCGTTGCAACGACGCATCAATTGTTACGTTATTATAGAGCGTTTC
ATGTAATGATTGTAGATGAGATTGATGCCTTCCCGTATCATGCGGATCAAATGTTACAGTATGCAGTGCAGCAAGCGATG
AAAGAGAAAGCAGCGCGTATTTATTTAACTGCAACCCCTGATGAAAAGTGGAAGCGCAATTTCAGAACGGGGAAACAAAA
AGGTATCATTGTCTCTGGACGATACCATCGTCATCCGTTACCAGTTCCTCTATTTAGTTGGTGCGGAAATTGGAAGAAAA
GCCTCCATCATAAAAAAATTCCTCGTGTGTTACTACAATGGTTAAAAATGTACGTAAACAAAAAATACCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGAAATAGGCCTGTTATTGAAAGGGTTGGATCATAGAATCGATGGCGTACATGC
AGAAGATCCGATGAGAAAAGAAAAAGTGGAAGCGTTTCGAAAGGGAGACATTCCGTTATTAGTTACAACGACAATTTTAG
AAAGAGGGGTAACTGTGAAGAACTTACAAGTGGCTGTGTTAGGGGCTGAAGAAGAAATATTTTCAGAAAGTGCGCTCGTA
CAAATTGCAGGCCGAGCAGGTCGTAGTTTTGAAGAACCGTATGGTGAGGTTGTTTATTTTCATTACGGTAAGACAGAGTC
GATGGTACGTGCGAAAAGACATATTCAAAGTATGAACAAAAGTGCGAAAGAACAAGGATTAATTGATTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (108-254)

• Helicase conserved C-terminal domain (Helicase_C) (312-408)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	51.914	93.096	0.483
comFA	Latilactobacillus sakei subsp. sakei 23K	40.704	88.641	0.361