Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   HPG46_RS20725 Genome accession   NZ_CP053656
Coordinates   3999594..4000637 (-) Length   347 a.a.
NCBI ID   WP_171856356.1    Uniprot ID   -
Organism   Bacillus cereus strain CTMA_1571     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3994594..4005637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPG46_RS20680 (HPG46_20290) - 3995021..3995221 (-) 201 WP_000106081.1 YqzE family protein -
  HPG46_RS20685 (HPG46_20295) aroK 3995260..3995757 (-) 498 WP_171856355.1 shikimate kinase AroK -
  HPG46_RS20690 (HPG46_20300) - 3995878..3996528 (-) 651 WP_000183879.1 2OG-Fe(II) oxygenase -
  HPG46_RS20695 (HPG46_20305) comGG 3996704..3997075 (-) 372 WP_001231192.1 competence type IV pilus minor pilin ComGG -
  HPG46_RS20700 (HPG46_20310) comGF 3997072..3997542 (-) 471 WP_000923094.1 competence type IV pilus minor pilin ComGF -
  HPG46_RS20705 (HPG46_20315) comGE 3997512..3997814 (-) 303 WP_000829458.1 competence type IV pilus minor pilin ComGE -
  HPG46_RS20710 (HPG46_20320) comGD 3997807..3998262 (-) 456 WP_000810395.1 comG operon protein ComGD -
  HPG46_RS20715 (HPG46_20325) comGC 3998259..3998558 (-) 300 WP_001178696.1 comG operon protein ComGC -
  HPG46_RS20720 (HPG46_20330) comGB 3998570..3999601 (-) 1032 WP_147790581.1 comG operon protein ComGB -
  HPG46_RS20725 (HPG46_20335) comGA 3999594..4000637 (-) 1044 WP_171856356.1 competence protein ComGA Machinery gene
  HPG46_RS20730 (HPG46_20340) - 4000843..4001538 (+) 696 WP_000434117.1 helix-turn-helix domain-containing protein -
  HPG46_RS20735 (HPG46_20345) - 4001664..4001906 (+) 243 WP_000440711.1 DUF2626 domain-containing protein -
  HPG46_RS20740 (HPG46_20350) - 4002014..4003408 (+) 1395 WP_001094329.1 L-cystine transporter -
  HPG46_RS20745 (HPG46_20355) - 4003625..4003840 (-) 216 WP_001008320.1 DUF3912 family protein -
  HPG46_RS20750 (HPG46_20360) - 4004093..4004404 (+) 312 WP_001093243.1 hypothetical protein -
  HPG46_RS20755 (HPG46_20365) - 4004441..4004929 (-) 489 WP_000764252.1 hypothetical protein -
  HPG46_RS20760 (HPG46_20370) - 4005009..4005383 (-) 375 WP_086421360.1 nucleoside 2-deoxyribosyltransferase -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 39485.03 Da        Isoelectric Point: 9.0606

>NTDB_id=446019 HPG46_RS20725 WP_171856356.1 3999594..4000637(-) (comGA) [Bacillus cereus strain CTMA_1571]
MNEIESFANMILKEACRVQASDLHIVPRQKDVVVQLRIGKDLMTKYCIEKEFGEKLVSHFKFLASMDIGERRKPQNGSLY
LQMDGQEVYLRLSTLPTVYQESLVIRLHLQASIQPLSHLSLFPSTAKKLLSFLRYSHGLLVFTGPTGSGKTTTMYALLEV
IRKKKTRRIVTLEDPVEKRNDDVLQIQINEKAGITYEAGLKAILRHDPDVILVGEIRDEETAKIAIRASLTGHLVMTTLH
TNDARGAILRFMDFGITRQEIEQSLLAIAAQRLVELKCPFCKRKCSTLCKSMRQVRQASIYELLYGYELKQAIKEANGEC
VTYKHETLQSSIRKGYALGFLEEDVYV

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=446019 HPG46_RS20725 WP_171856356.1 3999594..4000637(-) (comGA) [Bacillus cereus strain CTMA_1571]
ATGAATGAAATTGAAAGCTTTGCGAATATGATTTTGAAAGAAGCGTGCAGGGTACAAGCGTCGGACTTACATATTGTGCC
CCGGCAGAAGGATGTAGTGGTTCAGCTGCGTATAGGAAAAGATTTAATGACGAAATATTGTATTGAAAAGGAATTTGGAG
AAAAACTTGTTTCACACTTTAAATTTTTAGCATCTATGGATATAGGGGAGAGGCGGAAGCCACAAAATGGTTCACTGTAT
TTACAAATGGATGGACAAGAAGTGTATTTGCGTCTTTCCACGCTTCCAACCGTATACCAAGAAAGTCTCGTTATTCGTCT
TCATTTACAAGCATCTATTCAGCCGTTATCTCATCTTTCGTTATTTCCAAGTACAGCGAAGAAACTACTTTCTTTTTTAC
GTTATTCTCATGGATTACTCGTATTTACTGGACCGACTGGTTCGGGGAAGACAACAACAATGTATGCATTATTAGAGGTA
ATTAGAAAAAAGAAAACACGTCGCATCGTTACACTGGAAGATCCAGTTGAAAAAAGAAATGACGATGTATTACAAATTCA
AATAAATGAAAAAGCAGGTATCACATATGAGGCTGGACTAAAGGCTATTTTGCGTCATGATCCAGATGTTATTTTAGTCG
GTGAAATTCGTGATGAAGAAACAGCGAAAATTGCTATAAGAGCAAGTTTGACTGGCCATTTAGTAATGACGACATTGCAT
ACGAATGATGCGAGAGGGGCGATACTTCGGTTCATGGATTTTGGCATAACGAGGCAAGAAATCGAACAATCTTTATTAGC
TATAGCTGCACAGCGACTTGTCGAATTAAAGTGTCCGTTTTGCAAAAGAAAGTGCTCAACTTTATGCAAATCAATGAGGC
AAGTAAGGCAAGCGAGTATTTATGAGTTGTTATATGGATATGAGTTAAAACAAGCGATTAAAGAAGCAAACGGGGAATGT
GTCACATACAAGCACGAAACATTACAATCTTCGATACGAAAAGGATACGCTTTAGGGTTTTTAGAAGAAGATGTTTATGT
TTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

57.925

100

0.579
  pilB Vibrio campbellii strain DS40M4

35.977

100

0.366