Detailed information NTG0445999

Bioinformatically predicted

Overview

• Name: addA
• Type: Machinery gene
• Locus tag: HPG46_RS05770
• Genome accession: NZ_CP053656
• Coordinates: 1115275..1119000 (+)
• Length: 1241 a.a.
• NCBI ID: WP_086421004.1
• Uniprot ID: -
• Organism: Bacillus cereus strain CTMA_1571
• Function: homologous recombination; plasmid transformation (predicted from homology) Homologous recombination

Genomic Context

Location: 1110275..1124000

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HPG46_RS05755 (HPG46_05315)	-	1110433..1111026 (+)	594	WP_000347516.1	TVP38/TMEM64 family protein	-
HPG46_RS05760 (HPG46_05320)	lepB	1111083..1111646 (+)	564	WP_000751894.1	signal peptidase I	-
HPG46_RS05765 (HPG46_05325)	addB	1111763..1115278 (+)	3516	WP_171855787.1	helicase-exonuclease AddAB subunit AddB	Machinery gene
HPG46_RS05770 (HPG46_05330)	addA	1115275..1119000 (+)	3726	WP_086421004.1	helicase-exonuclease AddAB subunit AddA	Machinery gene
HPG46_RS05775 (HPG46_05335)	-	1119013..1119300 (+)	288	WP_000718624.1	RNA polymerase alpha subunit C-terminal domain-containing protein	-
HPG46_RS05780 (HPG46_05340)	gerPF	1119338..1119553 (-)	216	WP_001141566.1	spore germination protein GerPF	-
HPG46_RS05785 (HPG46_05345)	-	1119596..1119982 (-)	387	WP_000902341.1	spore germination protein GerPE	-
HPG46_RS05790 (HPG46_05350)	gerPD	1119998..1120192 (-)	195	WP_001052802.1	spore germination protein GerPD	-
HPG46_RS05795 (HPG46_05355)	gerPC	1120199..1120813 (-)	615	WP_001070766.1	spore germination protein GerPC	-
HPG46_RS05800 (HPG46_05360)	gerPB	1120881..1121087 (-)	207	WP_001012512.1	spore germination protein GerPB	-
HPG46_RS05805 (HPG46_05365)	gerPA	1121102..1121323 (-)	222	WP_001111188.1	spore germination protein GerPA	-
HPG46_RS05810 (HPG46_05370)	-	1121420..1121599 (-)	180	WP_000462851.1	aspartyl-phosphate phosphatase Spo0E family protein	-
HPG46_RS05815 (HPG46_05375)	-	1121847..1122746 (+)	900	WP_001241728.1	fumarylacetoacetate hydrolase family protein	-
HPG46_RS05820 (HPG46_05380)	-	1122787..1123071 (-)	285	WP_000925338.1	hypothetical protein	-

Sequence

Protein

• Length: 1241 a.a.
• Molecular weight: 142544.84 Da
• Isoelectric Point: 4.8485

>NTDB_id=445999 HPG46_RS05770 WP_086421004.1 1115275..1119000(+) (addA) [Bacillus cereus strain CTMA_1571]
MIENWPKKPEGSQWTDDQWKAVVANGRDILVAAAAGSGKTAVLVERIIKKIINEENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSQHIRKQLSLLNKASISTIHSFCLQVIRGYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
DNTIFFELVDRYTSDRSDDDLQRMILALHTESRAHPNPEKWLDKLVEAYDVEGKTIEDLVYASYLLEDVKFQLETAEQHI
RKATELAMLPDGPAPRIETLQADLALLGTLSAAARESWTSVYEAMQNVSWQTLKRIKKSDYNEDIVKQVDSLRNKAKDEV
KKLQEELFSRRPESFLRDFQDMHPVLEKLVQLVKVFTERFQAMKRDKGMVDFTDLEHFCLQILSEQSEDGEMKPSAVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDSESEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTQEGLGGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGEEVGEIDYDADAELKLGASYPEGEDVAAELLCIQQTEEEVIDGEEGAEVEKAQLEARLM
AQRIKAMVDSGYEVYDRKTDSMRPVQYRDFVILLRSMPWAPQIMEELKLQGIPVYADLATGYFEATEVNIMMNVFRVIDN
PMQDIPLAAVLRSPIVGLNDEELATLRAHGKKGSFYEVMSSFLKGAPLEEEKELHDKLGWFYNLLQGWREFARQQSLSDL
IWKVYGETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMTI
HKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTLSQLAIKRKMKMELIAEEMRVLYVALTR
AKEKLILIGTVKDATKEMEKWLDAREHSEWLLPDHVRAGASCYLDWIAPSLYRHRDSEMLLELGQGSIPDEIYGYDTSWK
VEVVDGNTLLAPEPVQEEKQELLEALREKKAVPLQSERKDEVYDRLMWKYGYEEATSHRAKQSVTEIKRNYQSEEGSDNA
FIKKLRAPIKTRPRFMEKKGLTYAERGTAVHAVMQHVDLKKPITVEVLQEQIAGMVNKELLTFEQAEEIAVEKVISFFDS
DLGKRVLAAKSVEREVPFTMMLAAEEAYQDWQGESGESILVQGVIDCMIEEEDGITLIDFKTDTIEGKFPGGFEQAKPIL
EERYKVQLSLYAKALEKSLQHPVKEKCLYFFDGNHVIKVEG

Nucleotide

• Length: 3726 bp

>NTDB_id=445999 HPG46_RS05770 WP_086421004.1 1115275..1119000(+) (addA) [Bacillus cereus strain CTMA_1571]
ATGATAGAAAATTGGCCTAAAAAACCAGAAGGTAGTCAGTGGACAGATGACCAGTGGAAAGCCGTTGTAGCGAACGGGCG
TGATATTTTAGTTGCGGCTGCAGCAGGATCAGGTAAGACAGCAGTATTAGTTGAACGTATTATTAAAAAGATTATTAATG
AAGAGAATCCAGTCGATGTCGACCGCCTGCTCGTTGTAACATTTACGAATGCAGCGGCGCAAGAGATGAAAAACAGAATT
GGGGAAGCATTAGAAAAAGTATTAATTGATGAGCCTGGCTCTCAGCACATCCGAAAGCAACTGAGCTTATTAAATAAAGC
TTCCATTTCAACGATCCATTCATTTTGTTTACAAGTTATTAGAGGATACTATTACATGCTTGATGTTGATCCTCGTTTCC
GCATTGCGAATCAAACCGAAAATGAATTATTAAAAGAAGAAGTGTTAGATGACATATTAGAAGAAGAGTATGGAATAGAA
GATAATACGATATTCTTTGAACTCGTTGATCGTTATACGAGCGACCGTAGTGATGATGATTTACAACGTATGATTTTAGC
GCTTCATACAGAATCAAGAGCGCATCCAAATCCGGAAAAATGGCTCGATAAATTAGTAGAAGCATATGACGTCGAAGGAA
AGACAATTGAAGATTTAGTGTACGCCTCTTACTTATTAGAAGATGTGAAATTCCAGCTTGAAACAGCGGAACAGCATATT
CGTAAAGCAACTGAGCTCGCAATGCTTCCTGACGGTCCAGCGCCTCGCATTGAAACGCTGCAAGCAGATTTAGCTTTACT
TGGAACGTTATCAGCAGCTGCTCGTGAATCGTGGACAAGCGTGTATGAAGCGATGCAAAACGTATCGTGGCAAACGTTAA
AGCGCATTAAGAAAAGCGATTATAACGAGGATATTGTAAAACAAGTAGACTCTCTTCGTAATAAAGCAAAAGATGAAGTG
AAGAAATTACAAGAAGAGCTATTTAGCCGCAGGCCTGAAAGTTTCTTACGAGATTTTCAAGATATGCATCCTGTATTAGA
AAAGCTCGTTCAACTTGTAAAAGTATTTACAGAGCGTTTCCAAGCGATGAAGCGAGATAAAGGCATGGTCGATTTCACAG
ATTTAGAGCATTTCTGTTTACAAATTTTAAGTGAACAAAGTGAAGATGGTGAAATGAAGCCATCAGCAGTAGCACTTCAA
TATCGTAATAAATTTGCTGAAGTATTAGTCGATGAATATCAAGATACGAACTTCGTACAAGAATCAATTATTAAATTCGT
AACGAAAGATTCCGAGAGTGAAGGAAACTTATTCATGGTTGGTGACGTGAAGCAGTCGATTTATCGTTTCCGACTAGCAG
AACCAGGATTATTCTTAGGAAAGTATAAACGTTTCACACAAGAAGGATTAGGCGGCGGAATGAAAATTGACTTAGCGAAA
AACTTCCGTAGTCGTCATGAAGTGTTAGCGGGTACAAACTTTATCTTCAAACAAATTATGGGCGAAGAAGTTGGGGAAAT
TGATTACGATGCTGACGCTGAATTAAAGCTAGGTGCTAGCTATCCAGAAGGTGAAGATGTAGCAGCTGAACTATTGTGCA
TTCAGCAAACAGAAGAAGAAGTAATAGACGGTGAAGAAGGTGCGGAAGTAGAAAAGGCACAGCTTGAAGCACGTCTTATG
GCACAGCGCATTAAAGCGATGGTTGATTCAGGTTATGAAGTGTATGATCGTAAAACGGATAGTATGCGCCCTGTACAATA
CCGTGACTTCGTTATTTTACTTCGCTCGATGCCGTGGGCACCGCAAATTATGGAAGAGTTAAAATTGCAAGGAATTCCAG
TATACGCTGACCTTGCCACTGGTTACTTTGAAGCGACAGAAGTAAATATTATGATGAACGTATTCCGCGTTATTGATAAT
CCGATGCAAGATATTCCGCTTGCAGCAGTACTTCGTTCCCCAATCGTTGGATTAAATGATGAAGAACTTGCAACGCTTCG
TGCTCACGGGAAGAAAGGCTCGTTTTATGAAGTAATGAGCTCATTCTTAAAAGGAGCACCGCTTGAAGAAGAAAAAGAAC
TACATGATAAATTAGGATGGTTCTATAACTTACTGCAAGGATGGCGTGAATTCGCACGCCAACAGTCTCTTTCTGATTTA
ATTTGGAAAGTGTACGGTGAGACAGGTTATTATGACTTTGTCGGTGGTTTACCAGCTGGAAAGCAAAGGCAAGCAAACCT
GCGTGTACTATATGACCGCGCAAGACAATATGAAGCAACATCGTTTAGAGGACTATTCCGCTTCTTACGCTTTATTGAGC
GTATTTTAGAACGCGGTGATGATATGGGTACGGCGAGAGCTTTAGGTGAACAAGAAGATGTCGTTCGCATTATGACAATT
CATAAAAGTAAAGGACTTGAGTTCCCAGTCGTATTCGTCGCTGGACTTGGTCGTCGTTTTAATACGCAAGACTTAATGAA
ACGTTTCTTACTTCATAAAGACTTCGGTTTCGGTTCGCAATTTATCGATCCGCGTAAACGAATTAAATATACGACATTAT
CACAACTTGCAATTAAGCGTAAAATGAAAATGGAATTAATTGCGGAAGAAATGCGCGTCTTATACGTAGCGTTAACACGA
GCAAAAGAGAAGTTAATTTTAATTGGAACGGTTAAGGATGCAACTAAGGAAATGGAAAAATGGCTGGATGCGAGGGAGCA
TAGTGAATGGTTATTACCAGATCACGTACGTGCCGGAGCATCTTGTTATTTAGACTGGATTGCACCTTCCTTATATAGAC
ACCGTGATAGTGAAATGCTTCTTGAATTAGGGCAAGGAAGTATTCCAGATGAAATTTATGGGTATGACACTAGCTGGAAA
GTAGAAGTTGTTGACGGGAACACGTTACTTGCGCCAGAACCCGTTCAAGAAGAGAAACAAGAATTGTTAGAAGCACTTCG
TGAGAAAAAAGCTGTTCCCCTGCAAAGTGAACGAAAAGATGAAGTGTACGACAGGTTAATGTGGAAGTACGGATATGAGG
AAGCGACATCTCATCGTGCGAAGCAATCTGTTACAGAAATAAAGAGAAATTATCAATCTGAAGAAGGTAGCGATAACGCT
TTTATTAAAAAATTACGTGCACCAATTAAAACACGTCCTCGTTTTATGGAGAAAAAAGGGTTAACGTACGCAGAGCGAGG
AACAGCAGTCCATGCCGTTATGCAGCATGTTGATTTGAAGAAGCCGATTACAGTTGAAGTTCTTCAAGAGCAAATTGCTG
GAATGGTAAATAAAGAATTATTAACATTTGAACAAGCAGAAGAAATAGCAGTTGAAAAAGTGATTTCATTCTTTGACAGT
GACCTAGGTAAAAGGGTATTAGCGGCGAAAAGTGTTGAGCGTGAAGTACCATTTACGATGATGCTTGCAGCAGAAGAAGC
GTATCAAGATTGGCAAGGGGAGAGCGGGGAATCAATTCTTGTCCAAGGGGTTATCGACTGCATGATTGAAGAGGAAGATG
GTATTACTTTAATCGACTTTAAAACGGATACGATTGAAGGGAAGTTCCCGGGAGGATTCGAACAAGCGAAACCAATTTTA
GAAGAGCGATATAAAGTGCAGCTTTCGTTATATGCAAAGGCACTTGAGAAAAGCTTACAACATCCTGTGAAAGAGAAATG
TTTATACTTCTTTGATGGTAATCATGTTATAAAAGTTGAGGGATAG

Domains

Predicted by InterproScan.

• PD-(D/E)XK nuclease superfamily (PDDEXK_1) (1060-1231)

• UvrD/REP helicase N-terminal domain (UvrD-helicase) (14-465)

• UvrD-like helicase C-terminal domain (UvrD_C) (476-890)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
addA	Bacillus subtilis subsp. subtilis str. 168	53.408	100	0.537