Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   I3J23_RS18385 Genome accession   NZ_CP065159
Coordinates   3674943..3675377 (+) Length   144 a.a.
NCBI ID   WP_048367275.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain GXU-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3669943..3680377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3J23_RS18380 (I3J23_18380) - 3673443..3674654 (-) 1212 WP_088005496.1 cytochrome P450 -
  I3J23_RS18385 (I3J23_18385) nucA/comI 3674943..3675377 (+) 435 WP_048367275.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  I3J23_RS18390 (I3J23_18390) - 3675437..3676192 (+) 756 WP_003154084.1 YoaK family protein -
  I3J23_RS18395 (I3J23_18395) - 3676226..3676588 (-) 363 WP_003154082.1 hypothetical protein -
  I3J23_RS18400 (I3J23_18400) - 3676781..3678109 (-) 1329 WP_024085327.1 S8 family peptidase -
  I3J23_RS18405 (I3J23_18405) - 3678289..3678522 (+) 234 WP_046560201.1 hypothetical protein -
  I3J23_RS18410 (I3J23_18410) - 3678778..3679485 (+) 708 WP_003154076.1 poly-gamma-glutamate hydrolase family protein -
  I3J23_RS18415 (I3J23_18415) - 3679545..3679997 (+) 453 WP_015388205.1 OsmC family protein -
  I3J23_RS18420 (I3J23_18420) - 3680011..3680364 (-) 354 WP_003154073.1 multidrug efflux SMR transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15465.41 Da        Isoelectric Point: 7.3203

>NTDB_id=445872 I3J23_RS18385 WP_048367275.1 3674943..3675377(+) (nucA/comI) [Bacillus amyloliquefaciens strain GXU-1]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAAHAASRYDQVLYFPLSKYPETGNHIKAAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=445872 I3J23_RS18385 WP_048367275.1 3674943..3675377(+) (nucA/comI) [Bacillus amyloliquefaciens strain GXU-1]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCATGCTGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGCCGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAACAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCATCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGCTATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

55.645

86.111

0.479