Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   H7994_RS20975 Genome accession   NZ_CP065152
Coordinates   4328070..4329497 (+) Length   475 a.a.
NCBI ID   WP_000980557.1    Uniprot ID   -
Organism   Escherichia coli O150:H6 strain FEX669     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4323070..4334497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7994_RS20945 (H7994_20940) pduU 4323448..4323798 (+) 351 WP_196316014.1 propanediol utilization microcompartment protein PduU -
  H7994_RS20950 (H7994_20945) - 4323803..4324246 (+) 444 WP_001189438.1 EutP/PduV family microcompartment system protein -
  H7994_RS20955 (H7994_20950) tmaR 4324347..4324676 (-) 330 WP_000450409.1 DUF496 family protein -
  H7994_RS20960 (H7994_20955) yeeA 4324847..4325905 (-) 1059 WP_001200890.1 FUSC family protein -
  H7994_RS20965 (H7994_20960) sbmC 4326103..4326576 (-) 474 WP_001105376.1 DNA gyrase inhibitor SbmC -
  H7994_RS20970 (H7994_20965) dacD 4326695..4327861 (-) 1167 WP_024225461.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
  H7994_RS20975 (H7994_20970) sbcB 4328070..4329497 (+) 1428 WP_000980557.1 exodeoxyribonuclease I Machinery gene
  H7994_RS20980 (H7994_20975) plaP 4329571..4330929 (-) 1359 WP_000019194.1 putrescine/proton symporter PlaP -
  H7994_RS20985 (H7994_20980) yoeI 4330919..4330981 (-) 63 WP_010723108.1 membrane protein YoeI -
  H7994_RS20990 (H7994_20985) yeeY 4331196..4332125 (-) 930 WP_196316015.1 LysR substrate-binding domain-containing protein -
  H7994_RS20995 (H7994_20990) yeeZ 4332171..4332995 (-) 825 WP_000754740.1 SDR family oxidoreductase -
  H7994_RS21000 (H7994_20995) yoeB 4333078..4333332 (-) 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB -
  H7994_RS21005 (H7994_21000) yefM 4333329..4333580 (-) 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  H7994_RS21010 (H7994_21005) hisL 4333863..4333913 (+) 51 WP_001364200.1 his operon leader peptide -

Sequence

Protein


Download         Length: 475 a.a.        Molecular weight: 54455.73 Da        Isoelectric Point: 5.4700

>NTDB_id=445719 H7994_RS20975 WP_000980557.1 4328070..4329497(+) (sbcB) [Escherichia coli O150:H6 strain FEX669]
MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGEN
EAAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWR
GNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDA
DRLGINRQHCLDNLKILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVD
KRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1428 bp        

>NTDB_id=445719 H7994_RS20975 WP_000980557.1 4328070..4329497(+) (sbcB) [Escherichia coli O150:H6 strain FEX669]
ATGATGAATGACGGTAAGCAACAATCTACCTTTTTGTTTCACGATTACGAAACCTTTGGAACGCACCCCGCGTTAGATCG
CCCTGCACAGTTCGCAGCCATTCGCACTGATAACGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCG
CGGATGACTATTTACCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCACAGGAGGCACGGGCGAAAGGAGAAAAC
GAAGCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTT
CGACGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGC
GCTGGGATTTACTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGT
CTACCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGT
GTACGCCACTATTGCGATGGCGAAACTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGTAATA
AACACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGC
GGCAATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGA
CATTTCGCCATTACTGGAGCTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAGCCGATCTTGGCGATAACG
CCGCCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTACTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCC
GACCGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTAAAAATTCTGCGTGAAAATCCGCAAGTGCGTGAAAAAGT
GGTAGCGATATTCGCGGAAGCCGAACCGTTTACACCTTCAGATAACGTGGATGCACAGCTTTATAATGGCTTTTTCAGTG
ACGCCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCGCGTAATTTACCGGCACTGGATATCACTTTTGTTGAT
AAACGAATTGAAAAACTGTTGTTCAATTATCGAGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTG
GCTGGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGCTACGCTGATGAATTGCAGATGCTGGCACAACAGT
ATGCCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATCGTCTAA

Domains

Predicted by InterProScan.

Exonuclease C-terminal (ExoI_SH3)

(212-472)

Exonuclease (RNase_T)

(13-188)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

58.936

98.947

0.583