Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   H7994_RS04490 Genome accession   NZ_CP065152
Coordinates   897463..898140 (+) Length   225 a.a.
NCBI ID   WP_000178175.1    Uniprot ID   A0A0H2Z3H2
Organism   Escherichia coli O150:H6 strain FEX669     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 892463..903140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7994_RS04455 (H7994_04455) gspG 892943..893380 (+) 438 WP_001202877.1 type II secretion system major pseudopilin GspG -
  H7994_RS04460 (H7994_04460) gspH 893388..893897 (+) 510 WP_001076035.1 type II secretion system minor pseudopilin GspH -
  H7994_RS04465 (H7994_04465) gspI 893894..894271 (+) 378 WP_001041748.1 type II secretion system minor pseudopilin GspI -
  H7994_RS04470 (H7994_04470) gspJ 894264..894851 (+) 588 WP_000610632.1 type II secretion system minor pseudopilin GspJ -
  H7994_RS04475 (H7994_04475) gspK 894844..895827 (+) 984 WP_001058507.1 type II secretion system minor pseudopilin GspK -
  H7994_RS04480 (H7994_04480) gspL 895842..897005 (+) 1164 WP_001335091.1 type II secretion system protein GspL -
  H7994_RS04485 (H7994_04485) gspM 897002..897463 (+) 462 WP_001298207.1 type II secretion system protein GspM -
  H7994_RS04490 (H7994_04490) pilD 897463..898140 (+) 678 WP_000178175.1 prepilin peptidase GspO Machinery gene
  H7994_RS04495 (H7994_04495) bfr 898169..898645 (-) 477 WP_000675504.1 bacterioferritin -
  H7994_RS04500 (H7994_04500) bfd 898717..898911 (-) 195 WP_000289085.1 bacterioferritin-associated ferredoxin -
  H7994_RS04505 (H7994_04505) chiA 899080..901782 (-) 2703 WP_097317173.1 bifunctional chitinase/lysozyme -

Sequence

Protein


Download         Length: 225 a.a.        Molecular weight: 24870.75 Da        Isoelectric Point: 7.6364

>NTDB_id=445659 H7994_RS04490 WP_000178175.1 897463..898140(+) (pilD) [Escherichia coli O150:H6 strain FEX669]
MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHFWQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGAL
FLYFCFALTLSVIDFRTQLLPDKLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLVCHKEGLGYGDFK
LLAAAGAWCGWQTLPMILLIASLGGIGYAIVSQLLQRRTITTIAFGPWLALGSMINLGYLAWISY

Nucleotide


Download         Length: 678 bp        

>NTDB_id=445659 H7994_RS04490 WP_000178175.1 897463..898140(+) (pilD) [Escherichia coli O150:H6 strain FEX669]
ATGACAATGCTACTGCCGCTCTTCATTCTGGTTGGTTTTATTGCAGGTTATTTTGTTAATGTCATGGCCTATCACCTCTC
GCCTTTGGAGGATAAAACGGCGTTAACGTTTCGCCAGGTATTGGTTCATTTCTGGCAAAAAAAATATGCCTGGCATGATA
CAGTGCCCCTGATACTTTGCGTTGCTGCTGCTATCGCCTGCGCCCTGGCACCTTTCACGCCCATTGTGACTGGCGCACTC
TTTCTCTATTTCTGTTTCGCACTCACGCTCAGTGTTATTGATTTTCGCACTCAGCTCCTGCCCGACAAACTCACCTTACC
GCTGCTCTGGCTTGGCTTGGTATTTAATGCGCAGTCTGGATTAATTGATTTACATGATGCGGTTTATGGCGCGGTCGCTG
GCTATGGGGTGCTGTGGTGTGTTTACTGGGGCGTCTGGTTAGTTTGTCACAAAGAGGGATTGGGCTACGGTGATTTCAAG
CTACTGGCCGCTGCAGGCGCGTGGTGTGGCTGGCAAACGTTGCCAATGATACTGCTGATTGCCTCGCTGGGTGGCATTGG
TTACGCCATCGTTTCACAACTTCTGCAACGCCGGACTATAACCACTATCGCATTCGGCCCGTGGCTTGCGCTCGGCAGCA
TGATAAACCTGGGATATCTGGCCTGGATCTCTTATTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(82-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2Z3H2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Neisseria gonorrhoeae MS11

33.333

100

0.387
  pilD Vibrio cholerae strain A1552

34.661

100

0.387