Detailed information NTG0445645

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: H7994_RS00895
• Genome accession: NZ_CP065152
• Coordinates: 149938..150930 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli O150:H6 strain FEX669
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 144938..155930

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H7994_RS00875 (H7994_00875)	ygbM	145572..146348 (+)	777	WP_196316080.1	HPr family phosphocarrier protein	-
H7994_RS00880 (H7994_00880)	ygbN	146437..147801 (+)	1365	WP_000104433.1	GntP family transporter	-
H7994_RS00885 (H7994_00885)	-	147944..148987 (-)	1044	WP_001161573.1	MBL fold metallo-hydrolase	-
H7994_RS00890 (H7994_00890)	-	149212..149763 (+)	552	WP_001179442.1	flavodoxin family protein	-
H7994_RS00895 (H7994_00895)	rpoS	149938..150930 (-)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
H7994_RS00900 (H7994_00900)	nlpD	150993..152087 (-)	1095	WP_306174120.1	murein hydrolase activator NlpD	-
H7994_RS00905 (H7994_00905)	pcm	152272..152898 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
H7994_RS00910 (H7994_00910)	surE	152892..153653 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
H7994_RS00915 (H7994_00915)	truD	153634..154683 (-)	1050	WP_097403403.1	tRNA pseudouridine(13) synthase TruD	-
H7994_RS00920 (H7994_00920)	ispF	154680..155159 (-)	480	WP_001219238.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
H7994_RS00925 (H7994_00925)	ispD	155159..155869 (-)	711	WP_021524451.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=445645 H7994_RS00895 WP_000081550.1 149938..150930(-) (rpoS) [Escherichia coli O150:H6 strain FEX669]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=445645 H7994_RS00895 WP_000081550.1 149938..150930(-) (rpoS) [Escherichia coli O150:H6 strain FEX669]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGCTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTGATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATCGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCACGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAACGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAATGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAATAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715