Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MBR110_RS03965 Genome accession   NZ_CP053609
Coordinates   850631..852136 (-) Length   501 a.a.
NCBI ID   WP_045578772.1    Uniprot ID   -
Organism   Burkholderia sp. MBR-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 845631..857136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MBR110_RS03940 (MBR110_03970) - 846658..847296 (-) 639 WP_011886060.1 LON peptidase substrate-binding domain-containing protein -
  MBR110_RS03945 (MBR110_03975) rapZ 847324..848232 (-) 909 WP_034192566.1 RNase adapter RapZ -
  MBR110_RS03950 (MBR110_03980) hprK 848308..849276 (-) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  MBR110_RS03955 (MBR110_03985) - 849406..849918 (-) 513 WP_011886058.1 PTS sugar transporter subunit IIA -
  MBR110_RS03960 (MBR110_03990) hpf 850113..850472 (-) 360 WP_011886057.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  MBR110_RS03965 (MBR110_03995) rpoN/rpoN1 850631..852136 (-) 1506 WP_045578772.1 RNA polymerase factor sigma-54 Machinery gene
  MBR110_RS03970 (MBR110_04000) lptB 852299..853075 (-) 777 WP_014723666.1 LPS export ABC transporter ATP-binding protein -
  MBR110_RS03975 (MBR110_04005) lptA 853072..853737 (-) 666 WP_011886054.1 lipopolysaccharide transport periplasmic protein LptA -
  MBR110_RS03980 (MBR110_04010) lptC 853758..854372 (-) 615 WP_011886053.1 LPS export ABC transporter periplasmic protein LptC -
  MBR110_RS03985 (MBR110_04015) - 854376..854912 (-) 537 WP_011886052.1 HAD family hydrolase -
  MBR110_RS03990 (MBR110_04020) kdsD 854912..855895 (-) 984 WP_014723665.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54828.98 Da        Isoelectric Point: 4.8247

>NTDB_id=445537 MBR110_RS03965 WP_045578772.1 850631..852136(-) (rpoN/rpoN1) [Burkholderia sp. MBR-1]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLESDDEWIASPLRVAADGSLIAQTPPAPNGESGS
MNGSGQSSERAERDEPAATDEYDGYGSGDTSDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLREHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTASLDEVLADLPPELEVDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKQLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVMP
RLRINNLYANILRNSRGDPSTGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALRIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=445537 MBR110_RS03965 WP_045578772.1 850631..852136(-) (rpoN/rpoN1) [Burkholderia sp. MBR-1]
ATGAAAGCCAGCCTCCAACTCCGCCTGTCGCAACACCTTGCGTTGACGCCGCAGCTGCAGCAGTCGATTCGGCTTTTGCA
GCTGTCGACGCTCGAGCTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAATCCGCTGCTGGAAAGCGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCAGCCGACGGATCGCTGATCGCGCAGACGCCGCCCGCCCCGAACGGCGAGTCCGGCAGC
ATGAACGGCAGCGGCCAGTCGAGCGAGCGCGCCGAACGCGACGAGCCGGCCGCCACCGACGAATACGACGGCTATGGCTC
GGGCGACACGAGCGACGGCCCGCAATGGAATCTCGACGAATTCGGCCGCTCGAGCGGCGCGTCGGACGACGACGATCTCC
CGCCGCTGCAGGTCCAGGAAGCCGCCACGTCGCTGCGCGAGCATCTGTCCGCGCAGCTGCGCGTCACGCAGGCCAGTCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCGTCGCTCGACGAAGTGCT
GGCCGACCTGCCGCCCGAACTCGAAGTCGACTGCGACGAGCTGAATGCGGCGCTCGCGCTGCTGCACAGCTTCGATCCGG
CCGGCGTCGGCGCGCGCTCGGCGTCCGAATGCCTGAAGCTGCAACTGCTGCGGCTCGATCCGTCCCCGACCCGCACGCTC
GCGCTGGAAATCGTCTCGCAGCATCTCGAGCTGCTCGCCGCGCGCGACTTCACGCGGCTGCGCAAGCAACTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCGGCCTACGGCAAGGCCGAGG
CCGACTACGTGGTACCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAGCTCAATCCGGAGGTGATGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATTCTGCGCAACAGCCGCGGCGATCCGTCGACCGGATCGTTGAAGCAACA
GCTGCAGGAAGCACGCTGGCTGATCAAGAATATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AACGTCAGAAGAACTTCTTTGCGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGTAAGTACATGCTGACCCCGTTCGGGACGCTGGAGTTCAAGTACTT
CTTCGGATCGCACGTTTCGACCGACACCGGAGGCGCCGCATCGTCGACCGCCATCCGAGCCCTGATCAAGCAACTGATAG
GAGCTGAAGACCCAAAGTCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGATTCGTGGTCGCGCGC
CGCACGGTCGCGAAGTATCGCGAAGCCCTCAGGATCCCGGCAGTGAATCTGCGCAAATCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

67.836

100

0.695