Detailed information NTG0445536

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: MBR110_RS03870
• Genome accession: NZ_CP053609
• Coordinates: 834353..835486 (-)
• Length: 377 a.a.
• NCBI ID: WP_011886072.1
• Uniprot ID: I2DQK4
• Organism: Burkholderia sp. MBR-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 829353..840486

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MBR110_RS03850 (MBR110_03880)	-	829833..830216 (+)	384	WP_011886076.1	nuclear transport factor 2 family protein	-
MBR110_RS03855 (MBR110_03885)	leuA	830357..832003 (-)	1647	WP_045578766.1	2-isopropylmalate synthase	-
MBR110_RS03860 (MBR110_03890)	ybiB	832452..833429 (-)	978	WP_011886074.1	DNA-binding protein YbiB	-
MBR110_RS03865 (MBR110_03895)	maiA	833624..834268 (+)	645	WP_011886073.1	maleylacetoacetate isomerase	-
MBR110_RS03870 (MBR110_03900)	pilA	834353..835486 (-)	1134	WP_011886072.1	signal recognition particle-docking protein FtsY	Machinery gene
MBR110_RS03875 (MBR110_03905)	rsmD	835688..836302 (+)	615	WP_011886071.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
MBR110_RS03880 (MBR110_03910)	coaD	836414..836911 (+)	498	WP_006398238.1	pantetheine-phosphate adenylyltransferase	-
MBR110_RS03885 (MBR110_03915)	-	836971..837237 (+)	267	WP_011886070.1	YfhL family 4Fe-4S dicluster ferredoxin	-
MBR110_RS03890 (MBR110_03920)	hisC	837308..838375 (-)	1068	WP_011886069.1	histidinol-phosphate transaminase	-
MBR110_RS03895 (MBR110_03925)	pth	838509..839108 (-)	600	WP_014723674.1	aminoacyl-tRNA hydrolase	-
MBR110_RS03900 (MBR110_03930)	-	839226..839831 (-)	606	WP_011886067.1	50S ribosomal protein L25/general stress protein Ctc	-

Sequence

Protein

• Length: 377 a.a.
• Molecular weight: 40148.96 Da
• Isoelectric Point: 5.0388

>NTDB_id=445536 MBR110_RS03870 WP_011886072.1 834353..835486(-) (pilA) [Burkholderia sp. MBR-1]
MFSFFKRFKKTQEPDSAESQPADAPQPDEPSAAPAPAARAADTARAPAVVMTVTPTNDGSDEVVEAVEIVPPPTQDASAK
KSWLSRLKSGLAKTGSSITSVFVNTKIDEDLYEELETALLMSDAGVDATEYLLGALREKVRVGRLTDPQQVKAALHDLLV
ELLQPLEKSLMLGRAQPLVMMITGVNGAGKTTSIGKLAKHLQSFDQSVLLAAGDTFRAAAREQLAVWGERNNVTVVQQES
GDPAAVIFDAVSAARARKIDVMMADTAGRLPTQLHLMEELKKVKRVIAKAHDGAPHEVLLVIDANTGQNALTQVKAFDDA
LGLTGLIVTKLDGTAKGGILAAIARQRPVPVYFIGVGEKVEDLQPFNAVEFADALLG

Nucleotide

• Length: 1134 bp

>NTDB_id=445536 MBR110_RS03870 WP_011886072.1 834353..835486(-) (pilA) [Burkholderia sp. MBR-1]
ATGTTCAGCTTCTTCAAACGATTCAAGAAAACGCAGGAGCCCGACTCCGCGGAATCGCAACCGGCCGACGCGCCGCAGCC
GGACGAGCCGTCCGCTGCGCCGGCCCCCGCCGCGCGAGCGGCGGACACTGCGCGCGCGCCCGCCGTCGTGATGACGGTCA
CGCCCACCAACGACGGCAGCGACGAAGTCGTCGAAGCGGTCGAGATCGTGCCGCCGCCGACGCAGGACGCGTCCGCGAAG
AAGTCCTGGCTGTCGCGCCTGAAATCGGGGCTCGCGAAGACGGGCTCGAGCATCACCAGCGTGTTCGTCAACACGAAGAT
CGACGAGGACCTGTACGAAGAACTCGAAACCGCGCTGCTGATGTCCGATGCCGGCGTCGATGCGACCGAATACCTGCTCG
GCGCGCTGCGCGAGAAGGTGCGCGTGGGCCGGCTGACCGACCCGCAGCAGGTGAAGGCGGCGCTGCACGATCTGCTCGTC
GAGCTGCTGCAGCCGCTCGAGAAATCGTTGATGCTCGGCCGCGCGCAGCCGCTCGTGATGATGATCACCGGCGTGAACGG
CGCGGGCAAGACGACCAGCATCGGCAAGCTCGCGAAGCATCTGCAGAGCTTCGACCAGTCGGTGCTGCTGGCCGCGGGCG
ACACGTTCCGCGCGGCCGCGCGCGAACAGCTGGCCGTCTGGGGCGAGCGCAACAACGTGACGGTCGTGCAGCAGGAAAGC
GGCGATCCGGCCGCGGTGATCTTCGATGCGGTCAGCGCCGCGCGCGCGCGCAAGATCGACGTGATGATGGCCGACACGGC
CGGCCGGTTGCCGACTCAGCTGCACCTGATGGAGGAGCTGAAAAAGGTGAAGCGCGTGATCGCGAAGGCGCACGACGGCG
CGCCGCACGAGGTGCTGCTCGTGATCGACGCGAACACCGGCCAGAACGCGCTCACGCAGGTGAAGGCCTTCGACGATGCG
CTCGGCCTCACCGGGCTGATCGTCACGAAGCTCGACGGCACCGCGAAGGGCGGGATCCTCGCCGCGATCGCGCGGCAGCG
GCCGGTGCCCGTCTACTTCATCGGCGTCGGCGAGAAGGTCGAGGACCTGCAGCCGTTCAACGCGGTGGAATTCGCGGACG
CGCTGCTCGGCTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (178-377)

• SRP54-type protein, helical bundle domain (SRP54_N) (95-157)

3D structure

• AlphaFold DB: I2DQK4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	52.885	100	0.584