Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   ITP52_RS19225 Genome accession   NZ_CP064818
Coordinates   3775226..3775636 (+) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain SH1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3770226..3780636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ITP52_RS19195 (ITP52_19195) arsR 3770391..3770708 (+) 318 WP_029318103.1 arsenical resistance operon transcriptional regulator ArsR -
  ITP52_RS19200 (ITP52_19200) arsK 3770767..3771207 (+) 441 WP_032722151.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  ITP52_RS19205 (ITP52_19205) acr3 3771230..3772270 (+) 1041 WP_032722149.1 arsenite efflux transporter Acr3 -
  ITP52_RS19210 (ITP52_19210) arsC 3772282..3772701 (+) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  ITP52_RS22315 - 3773056..3773234 (+) 179 Protein_3746 hypothetical protein -
  ITP52_RS19215 (ITP52_19215) spoIVCA 3773192..3774652 (+) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  ITP52_RS19220 (ITP52_19220) - 3774683..3775030 (-) 348 Protein_3748 sigma-70 family RNA polymerase sigma factor -
  ITP52_RS19225 (ITP52_19225) nucA/comI 3775226..3775636 (+) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  ITP52_RS19230 (ITP52_19230) yqeB 3775669..3776391 (-) 723 WP_032726205.1 hypothetical protein -
  ITP52_RS19235 (ITP52_19235) gnd 3776643..3777536 (+) 894 WP_041850033.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  ITP52_RS19240 (ITP52_19240) yqeD 3777556..3778182 (-) 627 WP_041850032.1 TVP38/TMEM64 family protein -
  ITP52_RS19245 (ITP52_19245) cwlH 3778369..3779121 (+) 753 WP_021480067.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  ITP52_RS19250 (ITP52_19250) yqeF 3779373..3780104 (+) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  ITP52_RS19255 (ITP52_19255) - 3780409..3780549 (-) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=445058 ITP52_RS19225 WP_009967785.1 3775226..3775636(+) (nucA/comI) [Bacillus subtilis strain SH1]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=445058 ITP52_RS19225 WP_009967785.1 3775226..3775636(+) (nucA/comI) [Bacillus subtilis strain SH1]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCAATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529